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  • Effects of the pore-forming agent nystatin on giant phospholipid vesicles
    Kristanc, Luka ; Svetina, Saša, 1935- ; Gomišček, Gregor
    The effects of the polyene pore-forming agent nystatin were investigated on individual giant unilamellar phospholipid vesicles (GUVs), made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), ... in different methanol-water solutions using phase-contrast optical microscopy. Three characteristic effects were detected in three different nystatin concentrationranges: vesicle shape changes (between 150 and 250muM); transient, nonspecific, tension pores (between 250 and 400muM); and vesicle ruptures (above 400muM). Both the appearance of the transient tension pores and the vesicle ruptures were explained as being a consequence of the formation of size-selective nystatin channels, whose membrane area density increases with the increasing nystatin concentrations. Our results also show that nystatin is able to form pores in the absence of sterols. In addition, study of the cross-interactions between nystatin and methanol revealed mutually antagonizing effects on the vesicle behavior for methanol volume fractions higher than 10%.
    Vir: Biochimica et biophysica acta. Biomembranes. - ISSN 0005-2736 (Vol. 1818, issue 3, 2012, str. 636-644)
    Vrsta gradiva - članek, sestavni del
    Leto - 2012
    Jezik - angleški
    COBISS.SI-ID - 29395161
    DOI

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