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Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkersKočevar Britovšek, Nina, 1984- ...Aim: To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma. Methods: We used two-dimensional gel electrophoresis and mass spectrometry to search for ... differentially expressed proteins in patients with gastric adenocarcinoma. A set of proteins was validated with immunoblotting. Results: We identified 30 different proteins involved in various biological processes: metabolism, development, death, response to stress, cell cycle, cell communication, transport, and cell motility. Eight proteins were chosen for further validation by immunoblotting.Our results show that gastrokine-1, 39S ribosomal protein L12 (mitochondrial precursor), plasma cell-induced resident endoplasmic reticulum protein, and glutathione S-transferase mu 3 were significantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples. On the other hand, septin-2, ubiquitin-conjugating enzyme E2 N, and transaldolasewere significantly overexpressed. Translationally controlled tumor protein was shown to be differentially expressed only in patients with cancer of the gastric cardia/esophageal border. Conclusion: This work presentsa set of possible diagnostic biomarkers, validated for the first time.It might contribute to the efforts of understanding gastric cancer carcinogenesis.Vir: World Journal of Gastroenterology = Shijie Wei-Changbingxue Zazhi. - ISSN 1007-9327 (Vol. 18, iss. 11, Mar. 2012, str. 1216-1228)Vrsta gradiva - članek, sestavni delLeto - 2012Jezik - angleškiCOBISS.SI-ID - 29696985
Avtor
Kočevar Britovšek, Nina, 1984- |
Odreman Macchioli, Federico |
Vindigni, Alessandro |
Frković-Grazio, Snježana |
Komel, Radovan
Teme
Stomach Neoplasms |
Diagnosis |
Adenocarcinoma |
Neoplasm Proteins |
Electrophoresis, Gel, Two-Dimensional |
Spectrum Analysis, Mass |
Immunoblotting |
Tumor Markers, Biological |
Image Processing, Computer-Assisted |
Adenokarcinom |
Novotvorba, beljakovine |
Spektralna analiza masna |
Želodec, novotvorbe |
Elektroforeza gelska, dvodimenzionalna |
Tumorski označevalci biološki |
Imunoblot |
Slika, obdelava z računalnikom
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Kočevar Britovšek, Nina, 1984- | 29388 |
Odreman Macchioli, Federico | ![]() |
Vindigni, Alessandro | ![]() |
Frković-Grazio, Snježana | 13983 |
Komel, Radovan | 06135 |
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