Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers
Kočevar Britovšek, Nina, 1984- ...
Aim: To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma. Methods: We used two-dimensional gel electrophoresis and mass spectrometry to search for ...differentially expressed proteins in patients with gastric adenocarcinoma. A set of proteins was validated with immunoblotting. Results: We identified 30 different proteins involved in various biological processes: metabolism, development, death, response to stress, cell cycle, cell communication, transport, and cell motility. Eight proteins were chosen for further validation by immunoblotting.Our results show that gastrokine-1, 39S ribosomal protein L12 (mitochondrial precursor), plasma cell-induced resident endoplasmic reticulum protein, and glutathione S-transferase mu 3 were significantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples. On the other hand, septin-2, ubiquitin-conjugating enzyme E2 N, and transaldolasewere significantly overexpressed. Translationally controlled tumor protein was shown to be differentially expressed only in patients with cancer of the gastric cardia/esophageal border. Conclusion: This work presentsa set of possible diagnostic biomarkers, validated for the first time.It might contribute to the efforts of understanding gastric cancer carcinogenesis.
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