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  • Diagnostic assays for crimean-Congo hemorrhagic fever
    Vanhomwegen, Jessica ...
    Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-bornedisease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, ... performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofl uorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, inhouse methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specifi city was excellent for all assays; molecular test results were infl uenced by patient country of origin. Our fi ndings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material.
    Vir: Emerging infectious diseases. - ISSN 1080-6040 (Vol. 18, no. 12, Dec. 2012, str. 1958-1965)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2012
    Jezik - angleški
    COBISS.SI-ID - 30341081
    DOI

vir: Emerging infectious diseases. - ISSN 1080-6040 (Vol. 18, no. 12, Dec. 2012, str. 1958-1965)
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