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  • Viability of human articular chondrocytes harvested postmortem : changes with time and temperature of in vitro culture conditions
    Alibegović, Armin ...
    Different studies of long-term chondrocytes viability have shown a gradual reduction as a function of time and ambient temperature. The aim of our in vitro study was to establish chondrocyte ... postmortem viability curves for 4°C, 11°C, 23°C, 35°C during 63 days after the donors' death. Osteochondral cylinders were procured from the knees of 16 male donors (2047 years), stored in preservation media that was not changed, and analyzed in 3-day intervals using a confocal laser scanning microscope. A significant influence of time on viability was found from Day 9 (p = 0.0029) and onwards (p < 0.0001). The lowest overall chondrocyte viability was at 35°C, followed by 4°C (p < 0.0001). The conditions used in this in vitro analysis suggest that similar viabilities may occur while in situ in the decedent. Further studies of chondrocyte viability from individuals with known postmortem intervals may show premise to help evaluate time since death in the late postmortem interval.
    Vir: Journal of forensic sciences. - ISSN 0022-1198 (Vol. 58, iss. 3, Mar. 2014, str. 325-332)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2014
    Jezik - angleški
    COBISS.SI-ID - 31150809

    Povezava(-e):

    http://onlinelibrary.wiley.com/doi/10.1111/1556-4029.12330/abstract;jsessionid=6E9BDCF3D53806E0A446CBB3573B5893.f03t01
    DOI

vir: Journal of forensic sciences. - ISSN 0022-1198 (Vol. 58, iss. 3, Mar. 2014, str. 325-332)
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