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Novosti u serološkoj i molekularnoj dijagnostici hepatitisa B i C = Recent developments in serologic and molecular diagnosis of hepatitis B and CPoljak, Mario, 1965- ; Židovec-Lepej, Snježana ; Đaković-Rode, OktavijaThe 2013 Update of the Croatian Guidelines for the Diagnosis and Treatment of Viral Hepatitis summarizes recent developments in the diagnosis of hepatitis B and C. Determination of HBsAg, anti-HBc ... and anti-HBs is the initial step in the diagnostic workup of acute and chronic hepatitis B. Other hepatitis B serologic markers should be analyzed in the second stage of the diagnostic workup in HBsAg and/or anti-HBc positive patients. A positive anti-HBc finding should be followed by HBV DNA quantification. HBsAg quantification is complimentary to the HBV DNA quantification and is used: (i) to differentiate between inactive HBsAg carriers and active chronic HBeAg-negative hepatitis B in patients with HBV DNA <2000 IU/mL; and (ii) for treatment monitoring in patients with chronic hepatitis B receiving pegylated interferon-alpha. Real-time PCR remains the method of choice for detection and quantification of HBV DNA. The first step in HCV testing is determination of specific antibodies via screening assays, enzyme immunoassays or point-of-care assays. All persons with positive results of anti-HCV screening assays should be additionally tested for HCV RNA or presence of HCV viral capsid antigen. Confirmatory anti-HCV assays should be used as additional assays for confirmation of reactive results obtained by screening enzyme immunoassays in HCV RNA-negative persons only. Molecular assays with identical lower limit of detection (LLOD) and lower limit of quantification are recommended for monitoring of viral kinetics during chronic hepatitis C triple therapy. HCV resistance testing to protease inhibitors is not part of the recommended diagnostic monitoring of patients receiving triple therapy. HCV subtyping is currently not recommended as part of pretreatment diagnostic algorithm due to currently insufficient evidence on its clinical usefulness. IL-28 genotype is an important predictor of SVR in patients treated with a combination of interferon-alpha and ribavirin as well as in patients with HCV genotype 1 receiving triple therapy. IL-28B genotyping is recommended as part of pretreatment diagnostic workup in patients with chronic hepatitis C and is a particularly important parameter for recommending double versus triple therapy in treatment-naïve patients with chronic hepatitis C.Vir: Acta medica Croatica : časopis Hrvatske akademije medicinskih znanosti = the journal of the Croatian Academy of Medical Sciences. - ISSN 1330-0164 (Vol. 67, iss. 4, 2013, str. 281-290)Vrsta gradiva - članek, sestavni delLeto - 2013Jezik - hrvaškiCOBISS.SI-ID - 31212505
Avtor
Poljak, Mario, 1965- |
Židovec-Lepej, Snježana |
Đaković-Rode, Oktavija
Teme
hepatitis B |
hepatitis C |
diagnosis |
hepatitis B |
hepatitis C |
diagnostika
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vir: Acta medica Croatica : časopis Hrvatske akademije medicinskih znanosti = the journal of the Croatian Academy of Medical Sciences. - ISSN 1330-0164 (Vol. 67, iss. 4, 2013, str. 281-290)
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Poljak, Mario, 1965- | 11622 |
Židovec-Lepej, Snježana | ![]() |
Đaković-Rode, Oktavija | ![]() |
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in: SICRIS
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