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The structures of native phosphorylated chicken cystatin and of a recombinant unphosphorylated variant in solutionDieckmann, T... ...The solution structures of the phosphorylated form of native chicken cystatin and the recombinant variant AEF-S1M-M29I-M89L were determined by 2D, 3D and 4D-NMR. The structures turn out to be very ... similar, despite the substitutions and the phosphorylation of the wild-type. Their dominant feature is a five-stranded beta-sheet, which is wrapped around a five-turn alpha-helix, as shown by X-ray crystallographic studies of wild-type chicken cystatin. However, the NMR analysis shows that the second helix observed in the crystal is not present in solution. The phosphorylation occurs at S80, which is located in a flexible region. For this reason, very few effects on the structure are observed. Comparison of structures of the unphosphorylated variant and the wild-type shows small effects on H84 which is located in the supposed recognition site of the serine kinase. This recognition site appears to be well structured as a large loop-containing bulge of the beta-sheet. The N termini of both mutants, which contribute to a large extent to the binding to the proteinase, are very flexible. A loop structure involving the residues L7 to A10 as found in related inhibitors, such as in the kininogen domains 2 and 3, is not sufficiently populated to be observed.Vir: Journal of molecular biology. - ISSN 0022-2836 (Letn. 234, št. 4, 1993, str. 1048-1059)Vrsta gradiva - članek, sestavni delLeto - 1993Jezik - angleškiCOBISS.SI-ID - 3568345
Avtor
Dieckmann, T... |
Mitschang, L... |
Hofmann, M... |
Kos, Janko, 1959- |
Turk, Vito |
Auerswald, E... A. |
Jaenicke, R... |
Oschkinat, H...
Teme
Cystatins |
Chemistry |
Phosphoproteins |
Chemistry |
Amino acid sequence |
Chickens |
Models, molecular |
Molecular sequence data |
Nuclear magnetic resonance |
Protein structure, secondary |
Recombinant proteins |
Solutions |
Ultracentrifugation
Vnos na polico
Trajna povezava
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Kos, Janko, 1959- | 04648 |
Turk, Vito | 01085 |
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