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  • Surface ultrastructural observations of Aurelia aurita (Scyphozoa)
    Turk, Valentina ...
    Moon jellyfish (Aurelia aurita s.l.) is a widespread, well-studied scyphozoan that frequently blooms in the Northern Adriatic. It has bipartite life cycle comprising attached polyp and free swimming ... medusa, both of which were examined during our study. The surface of a jellyfish is covered with mucus layer, which provide a unique habitat for microorganisms. As 16S rDNA sequencing analyses examination of bacterial communities associated with jellyfish surfaces revealed a considerable bacterial diversity, we visualized the mucus and associated microbes to better understand the interactions between the jellyfish host and microbes. For that purpose, we used light and electron microscopy in combination with different sample preparation and staining protocols. Sections of the body wall showed a thick epithelial layer of epidermis and gastrodermis, covering the outer and inner surface of the polyp, respectively, and thin mesoglea in between both epithelia. Both, epidermis and a gastrodermis contain cnidocytes and glandular cells among the other cells. Histological staining confirms presence of abundant mucus around moon jelly polyps, which contains acidic and neutral mucins. The surface examined by scanning electron microscopy revealed epidermal ciliated cells, as well as nematocysts in different stages, both undischarged and discharge. The most prominent features of the tissue surface of adult jellyfish were cilia protruding from the epidermal cells and microvilli surrounded the base of each cilium. Mucus secretions were observed on all external surfaces in form of flocs. Despite thorough examination, the bacteria were not observed on the surface of epidermal cells. In order to further examine the presence of microbes in the mucus, we used transmission electron microscopy. These methods revealed considerable amount of various bacterial morphtypes, embedded in mucus sheets, and in the inner part of the polyp, where prokaryotic, eukaryotic microbes and viruses were also found.
    Vir: Abstract book [Elektronski vir] (Str. 64)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2017
    Jezik - angleški
    COBISS.SI-ID - 4451407

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