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Potent DNA gyrase inhibitors bind asymmetrically to their target using symmetrical bifurcated halogen bonds [Elektronski vir]Kolarič, Anja, farmacevtka ...Novel bacterial type II topoisomerase inhibitors (NBTIs) stabilize single-strand DNA cleavage breaks by DNA gyrase but their exact mechanism of action has remained hypothetical until now. We have ... designed a small library of NBTIs with an improved DNA gyrase-binding moiety resulting in low nanomolar inhibition and very potent antibacterial activity. They stabilize single-stranded cleavage complexes and, importantly, we have obtained the crystal structure where an NBTI binds gyrase-DNA in a single conformation lacking apparent static disorder. This directly proves the previously postulated NBTI mechanism of action and shows that they stabilize single-strand cleavage through asymmetric intercalation with a shift of the scissile phosphate. This crystal stucture shows that the chlorine forms a halogen bond with the backbone carbonyls of the two symmetry-related Ala68 residues. To the best of our knowledge, such a so-called symmetrical bifurcated halogen bond has not been identified in a biological system until now.Vir: Nature communications [Elektronski vir]. - ISSN 2041-1723 (Vol. 12, 8 Jan. 2021, str. 150-1-150-13)Vrsta gradiva - e-članekLeto - 2021Jezik - angleškiCOBISS.SI-ID - 46272259
Avtor
Kolarič, Anja, farmacevtka |
Germe, Thomas |
Hrast, Martina |
Stevenson, Clare E. M. |
Lawson, David M. |
Burton, Nicolas P. |
Vörös, Judit |
Maxwell, Anthony |
Minovski, Nikola |
Anderluh, Marko
Teme
biokemija |
DNK |
Escherichia coli |
zaviralci |
antibacterials |
DNA gyrase inhibitors |
drug discovery |
Staphylococcus aureus |
intercalators |
novel bacterial topoisomerase inhibitors (NBTIs) |
symmetrical bifurcated halogen bonds |
crystal structure |
novel NBTIs mechanism of action
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Kolarič, Anja, farmacevtka | 38261 |
Germe, Thomas | |
Hrast, Martina | 32036 |
Stevenson, Clare E. M. | |
Lawson, David M. | |
Burton, Nicolas P. | |
Vörös, Judit | |
Maxwell, Anthony | |
Minovski, Nikola | 29497 |
Anderluh, Marko | 21456 |
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