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  • Characterization of a protein conjugate using an asymmetrical-flow field-flow fractionation and a size-exclusion chromatography with multi-detection system
    Rebolj, Katja ; Pahovnik, David ; Žagar, Ema
    In this study we present detailed characterization of a protein-PEG conjugate using two separation techniques, that is, asymmetrical-flow field-flow fractionation (AF4) and size-exclusion ... chromatography (SEC), which were onlinecoupled to a series of successively connected detectors: an ultraviolet,a multiangle light-scattering, a quasi-elastic light-scattering, and a refractive-index detector (UV-MALS(QELS)-RI). Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used as a complementary characterization technique. The results of AF4 as well as SEC on two columns connected in series, with both separation techniques coupled to a multidetection system, indicate the uniform molar massand chemical composition of the conjugate, that is, the molar ratio of protein to PEG is 1/1, the presence of minute amounts of residual unreacted protein and the aggregates with the same chemical composition as that of the conjugate. Since the portion of aggregated species is smaller in the acetate buffer solution containing 5% sorbitol than in the acetate buffer solution with 200-mM sodium chloride, the former buffer solution is more suitable for conjugate storage. The separation using only one SEC column results in poorly resolved peaks of the PEGylated protein conjugate and the aggregates, whereas MALDI-TOF MS analysis reveal the presence of the residual protein, but not theaggregates
    Vir: Analytical chemistry. - ISSN 0003-2700 (Vol. 84, issue 17, 2012, str. 7374-7384)
    Vrsta gradiva - članek, sestavni del
    Leto - 2012
    Jezik - angleški
    COBISS.SI-ID - 5034522

    Povezava(-e):

    http://pubs.acs.org/doi/abs/10.1021/ac3010378
    DOI

vir: Analytical chemistry. - ISSN 0003-2700 (Vol. 84, issue 17, 2012, str. 7374-7384)
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