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  • The lytic life cycle of the bacteriophage GIL01 infecting Bacillus thuringiensis is activated by the gp6 protein [Elektronski vir]
    Pavlin, Anja, biologija, 1992- ...
    Bacteriophage GIL01 is a temperate phage that infects insect pathogen bacterium Bacillus thuringiensis widely used in agriculture. During the lysogenic cycle, it resides in the host as a dormant ... independent linear replicon. In contrast to most other temperate bacteriophages that maintain lysogeny by a dedicated phage repressor, GIL01 lysogeny is maintained by a host transcriptional factor LexA. The GIL01 lytic cycle is induced when host genome is severly damaged. In this thesis we show that in comparison to the lysogenic promoter activity the expression from the lytic promoter P3 is considerably delayed. We further examined the regulation of the P3 promoter. We show that LexA in conjunction with phage protein gp7 binds P3 promoter region to repress lytic functions. However, the key to establish the lysogenic/lytic switch is the small bacteriophage-born protein gp6. We used surface plasmon resonance spectroscopy to demonstrate that gp6 binds P3 promoter region overlapping the -35 promoter element and thus acts as a type II transcription activator. The bioinformatics analysis suggests that gp6 is homologous to LexA. We generated the gp6-DNA complex model and according to the model, purified the gp6 K38A mutant that was indeed unable to interact with its binding site at the P3 promoter and failed to activate transcription from P3. Thus, we provide the first study showing that the interplay between two LexA family members, with opposing functions, ensures the timely expression of a phage late genes.
    Vir: International Conference of Undergraduate Research [Elektronski vir] : Conference 2020 : Tuesday, 29 September - Wednesday, 30 September 2020 (1 spletni vir (1 datoteka PDF ([1] str.)))
    Vrsta gradiva - prispevek na konferenci ; neleposlovje za odrasle
    Leto - 2020
    Jezik - angleški
    COBISS.SI-ID - 50495747

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    https://icur2020.entegyapp.com.au/Page/28/495

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