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  • Purification of glycerol kinase by "Dye-Ligand" chromatography and hydrophobic interaction chromatography on bead-cellulose derivatives
    Žúbor, Vladimír ...
    ǂThe ǂcrude extract of cytosole enzymes was obtained from homogenized cells of Saccharomyces cerevisiae by partition. The enzyme was then isolated from the lower aqueous phase displaying higher ... glycerol kinase activity by dye-ligand chromatography on Cibacron Blue (CB) or Remazol Brilliant Blue R (RB)-derivatized bead-cellulose, ATP being the eluent. The specific activity of glycerol kinase rised more than 10 and 7-times after affinity dye-ligand chromatography and hydrophobic interaction chromatography, respectively. Glycerol kinase obtained by the latter method was purified by CB-bead cellulose. The final preparation maintained its enzymic activity without noticeable losses during a long-term storage at 4 °C in dark.
    Vir: Collection of Czechoslovak Chemical Communications = Collection des travaux chimiques de Tchécoslovaquie = Sbornik čechoslovackich chimičeskich rabot. - ISSN 0010-0765 (Vol. 58, no 2, Feb. 1993, str. 445-451)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 1993
    Jezik - angleški
    COBISS.SI-ID - 517145369
    DOI

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