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  • The regeneration of bean plants from meristem culture
    Benedičič, Desimira ; Ravnikar, Maja ; Gogala, Nada, 1937-2013
    We studied the growth and development of Phaseolus vulgaris L. cv. Zorin meristems in vitro. Special attention was paidto the influence of different types of cytokinins and their concentration on bud ... induction, shoot growth and callus formation. The appropriate conditions of seed germination were established in order to separate seeds with various internal seed diseases prior to meristem isolation. Basal media was supplemented with 0.1-10 micromol/l benzyladenine, or with 1-10 micromol/l6-(y, y-dimetylallyamino)-purine, or with combinations of benzyladenine and gibberellic acid or alpha-naphthalene acetic acid. Althoughthe buds developed on all media used, the regeneration of plants able to survive in soil was achieved only when meristems were isolated on basal medium supplemented with 1 and 5 mocromol/l benzyladenine or 6-(y, y-dimethylallylaminol)-purine and with 20 micromol/l benzyladenine and 1.4 micromol/l gibberellic acid. The most vigorous plants developed when 1 micromol/l 6-(y, y-dimethylallylamino)-purine was used for initial meristem culture and was further incorporated in the elongation media. According to these results, we worked out a system for meristem regeneration, elongation and rooting, and prepared the protocol for production of mature bean plants.
    Vir: Phyton : annales rei botanicae. - ISSN 0079-2047 (Let. 37, št. 1, 1997, str. 151-160)
    Vrsta gradiva - članek, sestavni del
    Leto - 1997
    Jezik - angleški
    COBISS.SI-ID - 7113177

vir: Phyton : annales rei botanicae. - ISSN 0079-2047 (Let. 37, št. 1, 1997, str. 151-160)
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