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  • Regeneracija ekstraokularne mišice podgane po toksični degeneraciji ali paralizi : doktorska disertacija
    Stirn-Kranjc, Branka
    The muscles that are responsible for eye movements are among the most structurally and functionally diverse skeletal muscles. Extraocular muscle fibres (EOM) exhibit considerable variation in muscle ... fibre types, their size,high mitochondrial content and innervationž contractile properties. We were interested in histochemical and immunohistochemical characteristics of normal rat EOM (m. rectus medialis), particulary after their toxic degeneration or paralysis. We considered that their regeneration must be a long lasting, slow process. Muscle degeneration was caused with the application of 0.3 ml 0.5% Marcaine into rat EOM and toxic paralysis with the injection of a therapeutic dose of 5IU of Botulinum A. The regenerating muscles were analyzed six weeks, five and ten months after the degeneration. The muscles in recovery were analyzed one, five and eight months after paralysis. At least five rats were used for each experimental group. Control muscle sections and the regenerates after toxic degeneration and paralysis were analyzed histochemically and the enzyme activity was also evaluated histophotometically for myosin adenosin triphosphatase (mATP-ase), succinate dehydrogenase (SDH), menadion linked alpha glycerophosphate dehydrogenase (GPDH) activity and for fibres with -2XžD myosin heavy chain (MHC). Immunohistochemical analysis was performed with specific antibodies to identify MHC-neo, -2A, -2B and -l. Gel electrophoresis of muscle homogenates was performed too. At least six distinct fibre types that are differentially distributed across orbital and global layers were identified. Most known myosin MHC isoforms, including neonatal and tissue specific extraocular MHC were expressed in adult EOM. The observed regeneration after toxic degeneration was not complete after ten months. Mild fibre hypertrophy and then mild fibre atrophy was present mostly in fibres with central nuclei. (Abstract truncated at 2000 characters).
    Vrsta gradiva - disertacija
    Založništvo in izdelava - Ljubljana : [B. Stirn Kranjc], 1997
    Jezik - slovenski
    COBISS.SI-ID - 71374848

Knjižnica/institucija Kraj Akronim Za izposojo Druga zaloga
MF, Centralna medicinska knjižnica, Ljubljana Ljubljana CMK na dom 2 izv.
Narodna in univerzitetna knjižnica, Ljubljana Ljubljana NUK v čitalnico 1 izv.
Univerzitetna knjižnica Maribor Maribor UKM v čitalnico 1 izv.
ni za izposojo 1 izv.
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