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  • Surface characteristics of digestive gland epithelium of a terrestrial isopoda porcellio scaber studied by SEM = Značilnosti površine epitelija prebavne žleze organizma Porcellio scaber preiskovane s SEM
    Millaku, Agron ...
    This work reports the use of scanning electron microscope (SEM) to study the surface characteristics of digestive gland epithelium (hepatopancreas) of terrestrial isopods. Epithelial surface was ... investigated by conventional scanning electron microscopy (SEM), focused ion beam (FIB)/SEM and light microscopy. We investigated shape of cells, extrusion of lipid droplets, shape and distribution of microvilli, presence of bacteria on the cell surface and intracellular infection. All together 22 animals were investigated. The majority of them (17 animals) had digestive gland epithelial cells of regular shape. The dome shaped cells were densely and homogenously covered by microvilli. The epithelium of these animals differs in the portion of cells which were extruding lipid droplets. On the surface of microvilli we regularly observed scarcely distributed bacterial cells of different shapes. We classify such epithelium as normal gland epithelium. Abnormal appearance was detected in five of 22 animals. Here we found animals with significantly altered shape of epithelial cells. In three animals, cells were either significantly smaller, partly or completely flat and sometimes pyramid like. On the microvillous border a thick layer of bacteria was detected. In places, the shape and size of microvilli was atered. In two other animals, the some epithelial cells were extremely large. Light micrographs reveal, that these cells contain large vacuoles filled with bacteria what is a characteristic of intracellular infection. We conclude that SEM investigation of morphological characteristics of digestive gland epithelium surface provides valuable information on surface morphology of biological samples.
    Vir: Program in knjiga povzetkov = Program and book of abstracts (Str. 50)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2009
    Jezik - angleški
    COBISS.SI-ID - 781738

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