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Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female miceUmek, Nejc ...Although the effect of type 1 diabetes (T1DM) on the histological phenotype of skeletal muscle, especially capillarisation, remains poorly understood, this study sought to elucidate the changes in ... skeletal muscle myosin heavy chain (MyHC) fibres and 3D capillary network characteristics in T1DM-induced mice. Female C57BL/6J-OlaHsd mice were categorized into streptozotocin (STZ)-induced diabetic (n = 12) and age-matched non-diabetic controls (n = 12). The muscle fibre phenotype of the soleus, gluteus maximus, and gastrocnemius muscles was determined by the expression of MyHC isoforms. In contrast, the capillaries of the gluteus maximus were assessed using immunofluorescence staining, confocal laser microscopy, and 3D image analysis. STZ-induced diabetic mice showcased elevated glucose levels, reduced body weight, and prolonged thermal latency, verifying the T1DM phenotype. In both T1DM and non-diabetic mice, the gluteus maximus and gastrocnemius muscles predominantly displayed fast-twitch type 2b fibres, with no significant differences noted. However, the soleus muscle in non-diabetic mice had a greater proportion of type 2a fibres and comparable type 1 fibre densities (26.2 ± 14.6 % vs 21.9 ± 13.5 %) relative to diabetic mice. T1DM mice showed reduced fibre diameters (P = 0.026), and the 3D capillary network analysis indicated a higher capillary length per muscle volume in the gluteus maximus than controls (P < 0.05). T1DM instigated notable changes in skeletal muscle, such as MyHC fibre type shifts, diminished fibre diameters, and augmented relative capillarisation, potentially attributed to muscle fibre atrophy. Our results underscore the precision of the 3D analytical technique in delineating skeletal muscle capillary architecture and advise caution when interpreting 2D data for capillary changes in T1DM.Vir: Biomolecules & biomedicine. - ISSN 2831-0896 (Vol. , iss. [ahead of print], 2023, str. 1-34)Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasleLeto - 2023Jezik - angleškiCOBISS.SI-ID - 171111427
Avtor
Umek, Nejc |
Pušnik, Luka, 1996- |
Ugwoke, Chiedozie Kenneth |
Šink, Žiga |
Horvat, Simon |
Janáček, Jiří |
Cvetko, Erika
Teme
type 1 diabetes mellitus (T1DM) |
skeletal muscle |
myosin heavy chain isoforms |
capillary network analysis |
muscle fibre composition |
3D image analysis |
sladkorna bolezen tipa 1 (T1DM) |
skeletna mišica |
izoforme težke verige miozina |
analiza kapilarnih mrež |
sestava mišičnih vlaken |
analiza 3D slike
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Avtor | Umek, Nejc ... |
Naslov | Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice |
Datum objave | 2024-02-05 |
COBISS.SI-ID | 171111427 |
Verzija objave v repozitoriju | Založnikova različica |
Licenca objave v repozitoriju | Creative Commons Priznanje avtorstva 4.0 Mednarodna |
Embargo | Takojšnja javna objava |
Projekti, iz katerih je bila financirana objava
Naziv | Akronim | Številka projekta | Financer |
---|---|---|---|
Molekularni mehanizmi razvoja in delovanja skeletne mišice | P3-0043-2020 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Large RI Project LM2023050 Czech-BioImaging | LM2023050 |
Ministry of Education, Youth and Sports of the Czech Republic |
|
European Regional Development Fund | CZ.02.1.01/0.0/0.0/18_046/0016045 |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Umek, Nejc | 51820 |
Pušnik, Luka, 1996- | 57868 |
Ugwoke, Chiedozie Kenneth | 56421 |
Šink, Žiga | 57304 |
Horvat, Simon | 10412 |
Janáček, Jiří | ![]() |
Cvetko, Erika | 10644 |
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