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  • Development and validation of a simple and sensitive size-exclusion chromatography method for quantitative determination of heparin in pharmaceuticals
    Radivojša Matanović, Maja ...
    Heparin is widely used as an anticoagulant for the treatment and prevention of various thrombotic diseases. However, due to its high anionic charge, heterogeneity in size distribution and high ... polarity, its analysis is very challenging. In this paper, a novel method based on size-exclusion chromatography (SEC) for quantitative determination of intact heparin in pharmaceuticals is presented. Analyses were performed on a BioSep-SEC-S 2000 column with L-arginine solution at pH 6.5 as mobile phase and UV detection at 210 nm. The proposed method was found to be selective, linear (R2 > 0.997) over the concentration range of 3.1 to 1222 [micro]g mL-1, with a limit of detection of 1.0 [micro]g mL-1. Intra-day and inter-day precision were below 5.% and inaccuracy expressed as bias did not exceed 6.5 %. The reported method is simple, selective, sensitive, and requires no laborious sample preparation, which makes it appropriate for routine quantitative analysis of heparin in pharmaceuticals.
    Vir: Acta pharmaceutica. - ISSN 1330-0075 (Vol. 65, no. 1, 2015, str. 43-52)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2015
    Jezik - angleški
    COBISS.SI-ID - 3809905

    Povezava(-e):

    http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=190193
    DOI

vir: Acta pharmaceutica. - ISSN 1330-0075 (Vol. 65, no. 1, 2015, str. 43-52)

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