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Kemijski inštitut, Ljubljana (KILJ)
  • A genetic vaccine that activates both innate and adaptive immunity
    Lonzarić, Jan ; Jerala, Roman
    Vaccination is one of the most effective uses of immunology in modern medicine. It can provide both therapeutic and preventive immunity against a number of common pathogens by means of immune memory. ... Modern research shows that for the vaccine to have maximum efficiency it needs to activate both the innate and the adaptive immune response. Toll-like receptors play an importantrole in the coordination of both responses, as they bind to pathogen associated molecular patterns and through two different signaling cascades activate and direct the innate and adaptive immune responses. We have built several DNA constructs that confer signaling pathway activation and specific antibody fonnation by expressing protein fusions of active TLR3 and TLR4 fragments with selected Helicobacter pylori antigens, thus ensuring colocalization of the immune response activation with antigen processing. Through confocal microscopy and immunodetection we have been able to confinn the expression of these constructs in HEK293 cells, and by means of a dual luciferase test we have measured IFN~ and NF-KB promotor activation upon stimulation of cells with the constructs. In addition to preventative vaccination against infectious diseases this type of vaccine is potentially useful in cancer immunotherapy.
    Vir: Abstract book : immune system : genes, receptors and regulation (Str. 88)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2011
    Jezik - angleški
    COBISS.SI-ID - 4773658

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