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Selective immobilization and detection of DNA on biopolymer supports for the design of microarraysKargl, Rupert ...DNA immobilization for the manufacturing of microarrays requires sufficient probe density, low unspecific binding and high interaction efficiency with complementary strands that are detected from ... solutions. Many of these important parameters are affected by the surface chemistry and the blocking steps conducted during DNA spotting and hybridization. This work describes an alternative method to selectively immobilize probes and to detect DNA on biocompatible, hydrophilic cellulose coated supports with low unspecific binding, high selectivity and appropriate sensitivity. It takes advantage of a relatively selective adsorption of water soluble polysaccharides on a solid cellulose matrix. Single strands of DNA were conjugated to this soluble polysaccharide and subsequently micro-spotted on solid cellulose thin films that were coated on glass and polymer slides. This resulted in adsorptively bound DNA-probes that were used to detect complementary, labelled DNA strands with different lengths and sequences by hybridization. The interaction of the DNA-conjugates with cellulose surfaces and the selectivity of hybridization were investigated by a quartz crystal microbalance with dissipation monitoring (QCM-D) and fluorescence scanning. The method of non-covalent immobilization of DNA probes on an uncharged, non-reactive, hydrophilic support lowers the unspecific binding and the number of handling steps required to conduct the experiments for the detection of DNA on microarrays. Simultaneously selectivity, hybridization efficiency and detection limits are maintained.Vir: Biosensors & bioelectronics. - ISSN 0956-5663 (Vol. 68, iss. 4, Jun. 2015, str. 437-441)Vrsta gradiva - članek, sestavni delLeto - 2015Jezik - angleškiCOBISS.SI-ID - 18387478
Avtor
Kargl, Rupert |
Vorraber, V. |
Ribitsch, Volker |
Köstler, Stefan |
Stana-Kleinschek, Karin |
Mohan, Tamilselvan
Teme
DNA mikromreže |
kremenova mikrotehtnica |
fluorescenčna detekcija |
hibridizacija |
adsorpcija |
DNA imobilizacija |
DNA-microarrays |
quartz crystal microbalance |
fluorescence detection |
hybridization |
adsorption |
DNA-immobilization
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Kargl, Rupert | 36303 |
Vorraber, V. | |
Ribitsch, Volker | 15077 |
Köstler, Stefan | |
Stana-Kleinschek, Karin | 07814 |
Mohan, Tamilselvan | 33768 |
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