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Analiza epidemije stafilokokne zastrupitve s hrano = Analysis of the epidemic of staphylococcal food poisoningGrmek-Košnik, Irena ...Background. Staphylococcal food poisoning, being the consequence of ingestion of food, contaminated with Staphylococcus aureus and/or it is stable enterotoxins (SE), is usually epidemic. Food is ... contaminated with staphylococci by a person preparing it, who is a carrier and/or has a staphylococcal skin infection. The diagnosis of poisoning is made when the patients have typical symptoms and SE or/and S. aureus is isolated from food. In our study we wanted to determine, which kind of food was contaminated in the epidemic of food poisoning, which SE were involved, were the cooks carriers who contaminated food and environment and whether S. aureus or SE were present in the patients vomit. Materials. Together 37 samples were cultivated, of which 11 were throat, nose and wound swabs of four cooks, one the patients vomit, five food samples and 20 environmental samples. Antimicrobial susceptibility was performed by use of disk diffusion method. SEwere detected with agglutination test SET RPLA (Oxoid) and genotypes determined by the pulsed-field gel electrophoresis. Result. In the epidemic from twelve samples thirteen S. aureus isolates were found; five in two cooks,six in food samples and two in the environment. In the patients vomit neither S.aureus nor its SE were found. Eight isolates produced SE, six of them SE A and D and two SE C. The first cook had S. aureus with SE A and D andgenotype a in her throat and on the wound, but the second cook had S. aureus without SE and with genotype b in her throat, wound and on the skin. Three food samples had S. aureus with SE A and D and genotype a, one of them had also S. aureus with SE C and genotype a, and two had S. aureus without SE and with genotypes c and d. In one environmental sample S. aureus with SE A and D and genotype a was found, but in the second one S. aureus with SE C and genotype f. (Abstract truncated at 2000 characters.)Vir: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 69, št. 6, jun. 2000, str. 439-443)Vrsta gradiva - članek, sestavni delLeto - 2000Jezik - slovenskiCOBISS.SI-ID - 11694809
Avtor
Grmek-Košnik, Irena |
Müller-Premru, Manica |
Seljak, Marija |
Pavlica, Tatjana |
Peternelj, Brigita |
Pohar, Majda |
Kolman, Jana
Teme
Staphylococcal food poisoning |
Epidemiology |
Microbiology |
Staphylococcus aureus |
Disease outbreaks |
Enterotoxins |
Electrophoresis, gel, pulsed-field |
Genotype |
Drug resistance, microbial |
zastrupitve |
hrana |
epidemija |
stafilokoki
vir: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 69, št. 6, jun. 2000, str. 439-443)
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