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  • S kalicivirusi povzročena epidemija gastroenteritisa v domu za starejše osebe = Human calicivirus outbreak of acute gastroenteritis in an aged-care facility
    Štrumbelj, Iztok ...
    Background. Human caliciviruses represent a genetically and antigenetically diverse group of single stranded RNA viruses associated with acute gastroenteritis in humans. In last two years the number ... of notified gastroenteric cases in Slovenia is increasing. From January till November 2002already 574 calicivirus cases have been confirmed. Majority of cases were observed in preschool and school children but no cases were described in the aged-care facility. Methods. An outbreak of gastroenteritis in an aged-care facility occured. After onset of the outbreak an epidemiological questionnaireand inspection of local conditions were realized. Stool samples from home residents were analysed to find out bacteriological and/or viral aetiology. Direct electron microscopy and RT-PCR assay was performed to detectcaliciviruses. Viral RNA was amplified using specific primers and PCR products were identified in hybridisation test. Results. The outbreak started suddenly on the second floor, where the attack rate was the highest On the other floors the illness started later and the attack rate was lower. Sixty-one (40,1%) residents from 152 became ill and additionally 15 (22, 4%) employees from 67. The outbreak ended after ten days. Electron microscopy or/and RT-PCR revealed Norovirus members of family Caliciviridae in 9 of 10 stool specimens. As determined by RT-PCR and hybridisation assay viruses corresponded to genogroup II, genetic cluster 1 (closely related to the Hawaiivirus) andgenetic cluster 4 (closely related to thelordsdale virus).
    Vir: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 72, št. 5, maj 2003, str. 279-282)
    Vrsta gradiva - članek, sestavni del
    Leto - 2003
    Jezik - slovenski
    COBISS.SI-ID - 16299225

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