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Določanje antigenov CD62P in CD63 s pretočnim citometrom za oceno aktiviranih trombocitov = Detection of activated platelets by flow cytometric analysis of antigens CD62P and CD63Kralj, JanaBackground. Planteles may become activated in a number of disorders such as atherosclerosis, thrombosis and cancer. Therefore, the detection of activated platelets might facilitate the identification ... of certain thrombotic disorders.Previous methods for assaing platelet activity have been indirect, and quantitation has been difficult, precluding their use in the clinical area. Flow cytometry is a promising new method, using monoclonal antibodies specific for a glycoprotein markers of platelet activation expresssed on the membrane surface following activation. Methods. Cell surface expression of granule membrane antigens P-selectin (CD62P) and lysosome antigen CD63 were examined by flow cytometry in 30 healthy donors. We studied the conditions to prevent artifactual induction of platelet activation. Results. The proportion of platelets expressing antigens CD62P and CD63 in blood samples with paraformaldehyde was lower (<5%) than in samples with theophyillin (< 27%). The concentration-effect relationship were determined for the ADP, adrenaline,thrombin and collagen induced expression of CD62P and CD63. Conclusions. The results suggest that 1% solution of paraformaldehyde should be used for preventing of artifactual induction of platelet activation in blood samples. Platelets are likely to become activated by different agonist in different clinical situations. Therefore, it seems necessary to detect of activated platelets by a panel of monoclonal antibodies.Vir: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 67, supl. 1, maj 1998, str. I-57-I-59)Vrsta gradiva - članek, sestavni delLeto - 1998Jezik - slovenskiCOBISS.SI-ID - 7943641
Avtor
Kralj, Jana
Teme
Platelet activation |
Drug effects |
Immunology |
P-selectin |
Platelet membrane glycoproteins |
Flow cytometry |
Antibodies, monoclonal |
Adenosine diphosphate |
Thrombin |
Collagen |
hematologija |
kri |
trombociti |
antigeni |
diagnostika
vir: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 67, supl. 1, maj 1998, str. I-57-I-59)
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