This study proposes a system that can collect and analyze the brake particle matter generated from a vehicle and brake dynamometer. A dust cover is developed to collect brake particle matter, and the ...number of particle matter by size is measured using a portable aerosol spectrometer. Brake PM
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generated when the vehicle is driving on highways, country and city roads is collected and analyzed. Based on the vehicle driving data, the test mode for the brake dynamometer is developed using the relationship between brake energy and brake power. The same brake fine dust collection and measurement system as the vehicle is installed on the brake dynamometer, and brake PM
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generated according to the test mode for each road condition is collected and analyzed. As a result, the total number of brake PM
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collected from the vehicle and brake dynamometer shows about maximum of 20% error rate for each road condition. The Pearson correlation coefficient for the test results is 0.9988, and it means that the vehicle and brake dynamometer test results has a strong positive relationship.
Tubulysins are a group of bioactive secondary metabolites from myxobacteria exhibiting strong anticancer activity against various cancer cell lines. In this study, we describe the identification of ...putative tubulysin biosynthetic gene clusters (tubA~tubF) in the genome sequences of two tubulysin-producing myxobacterial strains, Archangium gephyra MEHO_002 and MEHO_004. The inactivation of the putative tubulysin biosynthetic genes resulted in a tubulysin-production defect. The DNA sequences of the A. gephyra MEHO_002 and MEHO_004 tubulysin biosynthetic genes were 97% identical, and the amino acid sequences of the encoded proteins shared a similarity of 97−100%. The nucleotide sequences of the tubulysin biosynthetic gene clusters in MEHO_002 and MEHO_004 were 86% identical to that in Cystobacter sp. SBCb004 known as a tubulysin-producing myxobacterium, and the organization of the clusters was identical except for the lack of a tubZ gene in the clusters in MEHO_002 and MEHO_004. The amino acid sequences of the proteins encoded by each gene were 88-97% similar to those encoded by SBCb004, and the domain compositions of the proteins were also identical. KCI Citation Count: 0
Tubulysins are a group of secondary metabolites produced by myxobacteria that inhibit the function of the eukayotic cytoskeleton. We developed a pair of PCR primers that specifically amplified ...tubulysin biosynthetic genes. Using these primers, eight out of the eighty-one strains of myxobacteria belonging to the Cystobacteraceae family that harbored putative tubulysin biosynthetic genes were screened through PCR analysis. The selected strains included two Archangium gephyra, two Stigmatella sp., two Vitiosangium cumulatum, and two unidentified myxobacteria. LC-MS analysis of the culture extracts from the selected strains revealed that A. gephyra KYC4066 produced putative tubulysin A and B. KCI Citation Count: 1
Tubulysin은 다양한 암세포주에 대해 강한 항암활성을 보이는 점액세균 유래 이차대사 생리활성물질이다. 본 연구에서는 tubulysin을 생산하는 두 균주의 점액세균 Archangium gephyra MEHO_002와 MEHO_004의 유전체 분석을 통해 tubulysin 생합성 유전자들로 추정되는 유전자군을 발견하였으며, 플라스미드 삽입에 의한 ...유전자 불활성화를 통해 이들 유전자들이 tubulysin 생산과 직접 연관되어 있음을 확인하였다. A. gephyra MEHO_002와 MEHO_004 균주의 tubulysin 생합성 유전자군(tubA~tubF)은 DNA 염기서열이 서로 97% 동일하였으며, 암호화하는 단백질들의 아미노산 서열도 서로 97-100% 유사하였다. MEHO_002와 MEHO_004 균주의 tubulysin 생합성 유전자군은 tubulysin 생산 점액세균으로 알려진 Cystobacter sp. SBCb004의 tubulysin 생합성 유전자군과 DNA 염기서열이 86% 동일하였다. 유전자군의 구성은 tubZ 유전자가 존재하지 않는다는 점을 제외하고는 SBCb004의 tubulysin 생합성 유전자군 구성과 동일하였다. 각 유전자가 암호화하는 단백질의 아미노산 서열은 Cystobacter sp. SBCb004의 tubulysin 생합성 유전자가 암호화하는 단백질들과 88-97% 유사하였으며, 각 단백질들의 도메인 구성도 동일하였다.
Tubulysins are a group of bioactive secondary metabolites from myxobacteria exhibiting strong anticancer activity against various cancer cell lines. In this study, we describe the identification of putative tubulysin biosynthetic gene clusters (tubA~tubF) in the genome sequences of two tubulysin-producing myxobacterial strains, Archangium gephyra MEHO_002 and MEHO_004. The inactivation of the putative tubulysin biosynthetic genes resulted in a tubulysin-production defect. The DNA sequences of the A. gephyra MEHO_002 and MEHO_004 tubulysin biosynthetic genes were 97% identical, and the amino acid sequences of the encoded proteins shared a similarity of 97-100%. The nucleotide sequences of the tubulysin biosynthetic gene clusters in MEHO_002 and MEHO_004 were 86% identical to that in Cystobacter sp. SBCb004 known as a tubulysin-producing myxobacterium, and the organization of the clusters was identical except for the lack of a tubZ gene in the clusters in MEHO_002 and MEHO_004. The amino acid sequences of the proteins encoded by each gene were 88-97% similar to those encoded by SBCb004, and the domain compositions of the proteins were also identical.
본 연구는 교육과정기반 치료지원을 위한 특수교사-물리치료사간 협력적 팀 접근의 그동안의 문제점을 파악하여 새로운 협력방안을 도출하고 그에 따른 개선방안을 제시하는 것을 목적으로 실행하였다. 이를 위하여 특수교사, 물리치료사, 학부모로 구성된 21인의 전문가 델파이 조사와 AHP 기법을활용하여 수행되었다. 연구의 결과 첫째, 특수교사, 물리치료사, 제도적 ...측면의 주체별 구체적인 역할과 세부사항을 도출하여 최종 11가지 주제와 44문항을 확정하였다. 둘째, 도출된 내용을 토대로 교육과정기반 치료지원을 위한 특수교사-물리치료사간 협력적 팀 접근에 관한 협력방안 및 협력모형을도출하였다. 이상의 결과를 바탕으로 종합적으로 논의하고 제언 및 연구의 시사점을 제시하였다. 이연구를 토대로 향후 이러한 연구결과가 교육과정기반 치료지원을 위한 특수교사-물리치료사간 협력적 팀 접근 모델 개발 및 정책 수립 기초연구로 사용되기를 기대한다. The purpose of this study was to identify the problems of cooperative team approach between special teachers and physical therapists to support curriculum-based treatment, to derive new cooperative measures and to present improvement measures accordingly. To this end, 21 experts including special teachers, physical therapists, and parents were investigated using AHP techniques. As a result of the study, first, specific roles and details for each subject in the special teacher, physical therapist, and institutional aspects were derived to confirm the final 11 topics and 44 questions. Second, based on the results, the cooperative measures and cooperative models were derived for the cooperative approach between special teachers and physical therapists to support curriculum-based treatment. Based on the above results, we discuss comprehensively and present implications for suggestions and research. Based on this study, we hope that these findings will be used as a basic study of developing a cooperative team approach model and policy establishment between special teachers and physical therapists to support curriculum-based treatment. KCI Citation Count: 1
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