An accurate, sensitive, precise and simple method was developed utilizing Gas Chromatography for the quantitative analysis of benzyl isothicyanate in Siwak extract and dental care herbal products ...claimed to contain Siwak. Rtx (30.0 m × 0.25 mm ID, 25 µm thickness) column was used and helium as carrier gas at a flow rate of 0.74 mL/min. The retention time of standard benzyl isothicyanate was 13.470 min under the described conditions. Linear regression data analysis indicated a good linear relationship between peak height measurement and concentration of benzyl isothiocyanate in the range of 10–50 µg/ml (R2 = 0.9971). The regression equation was y = 11,471x. The developed GC method was subjected to validation requirements set by the ICH for precision, accuracy, and robustness. The entitled GC analyses expected to be valuable for the determination of benzyl isothiocyanate in Siwak extracts and other formulations containing Siwak extract. The amount of benzyl isothiocyanate reflects the efficacy of the products.
Anvillea garcinii
is a medicinal plant traditionally used for the treatment of dysentery, gastrointestinal troubles, hepatitis, lung disease, colds, digestive problems and pulmonary affections and in ...liver diseases. Four new sesquiterpene lactones, garcinamines A–D, along with seven known compounds, were isolated from the leaves of
A. garcinii
. This is the first report of the isolation of amino acid analogues of parthenolide-type sesquiterpene lactones from the family Asteraceae. Total ethanol extract of leaves as well as the chloroform and
n
-butanol fractions were tested for their hepatoprotective effect using the carbon tetrachloride liver toxicity model. The chloroform fraction, at a dose of 400 mg/kg, demonstrated a significant hepatoprotective effect comparable to silymarin in all serum and tissue parameters. The cytotoxicity of all extracts and compounds were evaluated against five human cancer cell lines: MCF-7, HCT-116, HepG2, Hela and A-549. The results indicated that the chloroform and
n
-butanol fractions and compounds
3
,
4
,
7
and
8
displayed significant cytotoxic activity against these cell lines.
Hepatoprotective Constituents from Cleome droserifolia Abdel-Kader, Maged Saad; Alqasoumi, Saleh Ibrahim; Al-Taweel, Areej Mohammad
Chemical & Pharmaceutical Bulletin,
06/2009, Letnik:
57, Številka:
6
Journal Article
Recenzirano
Odprti dostop
The effect of ethanol extract from aerial parts of Cleome droserifolia was investigated against carbon tetrachloride induced liver injury. The hepatoprotective activity was evaluated through the ...quantification of biochemical parameters and confirmed using histopathology analysis. Efficient hepatoprotective effect was achieved by crude extract, fractions and some pure compounds. The phytochemical studies showed that the petroleum ether fraction afforded two known guaiane sesquiterpenes buchariol (1) and teucladiol (2) in addition to daucosterol (β-sitosterol glucoside) (3). The CHCl3 fraction afforded three known flavonoid derivatives; 5,3′-dihydroxy-3,6,7,4′,5′-pentamethoxyflavone (4), 5′-hydroxy-3,6,7,3′,4′,5′-hexamethoxyflavone (5) and luteolin (6) and a known dolabellane diterpene (1R,2R,3E,7E,11R,12S)-2-O-acetyl-16-O-(3-hydroxy-3-methylglutaryl)-dolabella-3,7-dien-2,16,18-triol (7). The active parts of the MeOH fraction afforded the previously unreported 3′-methoxy-3,5,4′-trihydroxy flavone-7-neohesperidoside (8) and a known megastigmane norterpene; (6S,9R)-roseoside (9).
(AG, Asteraceae) commonly called “nougd” in Arabic is traditionally used for the treatment of different gastrointestinal disorders. This study evaluates the effects of ethanol, chloroform and ...n-butanol extracts of AG on ulcerative colitis induced by various ulcerogens in rats and the isolation of new secondary metabolites. Ethanol extracts of the leaf of the AG demonstrated a powerful anti-ulcer activity which could be attributed to the synergistic effect of its constituents. Therefore, our results would be the first proof of traditional use of AG as an antiulcer ailment.
The petroleum ether fraction of Juniperus procera showed significant activity as hepatoprotective when investigated against carbon tetrachloride induced liver injury. The hepatoprotective activity ...was evaluated through the quantification of biochemical parameters and confirmed using histopathology analysis. Phytochemical investigation of the petroleum ether fraction utilizing different chromatographic techniques resulted in the isolation of six known diterpenoids namely: 4-epi-abietol (1), ferruginol (2), hinokiol (3), sugiol (4), Z-communic acid (5) and hinokiol-1-one 3β,12-dihydroxyabieta-8,11,13-triene-1-one) (6), in addition to the sesquiterpene 8α-acetoxyelemol (7). Both physical and spectral data were used for structure determination and all isolates were evaluated for their hepatoprotective activity. Compounds 1 and 4 were the most effective in reducing the elevated liver enzymes as indication for liver protection.
Plants secondary metabolites undergoes qualitative and quantitative variation due to environmental and growth factors. It is a crucial factor to select the proper time for collection of medicinal ...plants to assure maximum content of active components reflected as maximum efficacy. Olive leaves (Olea europaea L.) are known traditionally for their antidiabetic effect. The secoiridoid glycoside oleuropein is the main active component of Olive leaves responsible for the biological activity. The current study was conducted to monitor the seasonal variation of oleuropein in Olives leaves collected from the same location. To achieve this goal a validated HPLC method following the ICH guidelines was established. Separation was conducted using RP18 column and a mobile phase consisted of ultrapure water containing 20% acetonitrile and 1% acetic acid. Detection was performed at 254 nm with 1 mL/min flow rate. The method was simple, linear, accurate, precise, specific and robust. The analyses revealed considerable variations in the level of oleuropein throughout the year. This variation cannot be explained by temperature variation during the year. Two points of high levels of oleuropein were detected prior to flowering stage and ripening of the fruits. The levels of growth regulators most likely is responsible for the increased production of oleuropein. It is recommended that leaves intended for medicinal use to be collected during the fruiting stage prior to fruit ripening.
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