Blind and Semi-Blind Deblurring of Natural Images Almeida, M.S.C.; Almeida, L.B.
IEEE transactions on image processing,
2010-Jan., 2010, 2010-Jan, 2010-01-00, 20100101, Letnik:
19, Številka:
1
Journal Article
Recenzirano
A method for blind image deblurring is presented. The method only makes weak assumptions about the blurring filter and is able to undo a wide variety of blurring degradations. To overcome the ...ill-posedness of the blind image deblurring problem, the method includes a learning technique which initially focuses on the main edges of the image and gradually takes details into account. A new image prior, which includes a new edge detector, is used. The method is able to handle unconstrained blurs, but also allows the use of constraints or of prior information on the blurring filter, as well as the use of filters defined in a parametric manner. Furthermore, it works in both single-frame and multiframe scenarios. The use of constrained blur models appropriate to the problem at hand, and/or of multiframe scenarios, generally improves the deblurring results. Tests performed on monochrome and color images, with various synthetic and real-life degradations, without and with noise, in single-frame and multiframe scenarios, showed good results, both in subjective terms and in terms of the increase of signal to noise ratio (ISNR) measure. In comparisons with other state of the art methods, our method yields better results, and shows to be applicable to a much wider range of blurs.
The alternating direction method of multipliers (ADMM) has recently sparked interest as a flexible and efficient optimization tool for inverse problems, namely, image deconvolution and reconstruction ...under non-smooth convex regularization. ADMM achieves state-of-the-art speed by adopting a divide and conquer strategy, wherein a hard problem is split into simpler, efficiently solvable sub-problems (e.g., using fast Fourier or wavelet transforms, or simple proximity operators). In deconvolution, one of these sub-problems involves a matrix inversion (i.e., solving a linear system), which can be done efficiently (in the discrete Fourier domain) if the observation operator is circulant, i.e., under periodic boundary conditions. This paper extends ADMM-based image deconvolution to the more realistic scenario of unknown boundary, where the observation operator is modeled as the composition of a convolution (with arbitrary boundary conditions) with a spatial mask that keeps only pixels that do not depend on the unknown boundary. The proposed approach also handles, at no extra cost, problems that combine the recovery of missing pixels (i.e., inpainting) with deconvolution. We show that the resulting algorithms inherit the convergence guarantees of ADMM and illustrate its performance on non-periodic deblurring (with and without inpainting of interior pixels) under total-variation and frame-based regularization.
Image deblurring (ID) is an ill-posed problem typically addressed by using regularization, or prior knowledge, on the unknown image (and also on the blur operator, in the blind case). ID is often ...formulated as an optimization problem, where the objective function includes a data term encouraging the estimated image (and blur, in blind ID) to explain the observed data well (typically, the squared norm of a residual) plus a regularizer that penalizes solutions deemed undesirable. The performance of this approach depends critically (among other things) on the relative weight of the regularizer (the regularization parameter) and on the number of iterations of the algorithm used to address the optimization problem. In this paper, we propose new criteria for adjusting the regularization parameter and/or the number of iterations of ID algorithms. The rationale is that if the recovered image (and blur, in blind ID) is well estimated, the residual image is spectrally white; contrarily, a poorly deblurred image typically exhibits structured artifacts (e.g., ringing, oversmoothness), yielding residuals that are not spectrally white. The proposed criterion is particularly well suited to a recent blind ID algorithm that uses continuation, i.e., slowly decreases the regularization parameter along the iterations; in this case, choosing this parameter and deciding when to stop are one and the same thing. Our experiments show that the proposed whiteness-based criteria yield improvements in SNR, on average, only 0.15 dB below those obtained by (clairvoyantly) stopping the algorithm at the best SNR. We also illustrate the proposed criteria on non-blind ID, reporting results that are competitive with state-of-the-art criteria (such as Monte Carlo-based GSURE and projected SURE), which, however, are not applicable for blind ID.
The cecropin-melittin hybrid antimicrobial peptide BP100 (H-KKLFKKILKYL-NH2) is selective for Gram-negative bacteria, negatively charged membranes, and weakly hemolytic. We studied BP100 ...conformational and functional properties upon interaction with large unilamellar vesicles, LUVs, and giant unilamellar vesicles, GUVs, containing variable proportions of phosphatidylcholine (PC) and negatively charged phosphatidylglycerol (PG). CD and NMR spectra showed that upon binding to PG-containing LUVs BP100 acquires α-helical conformation, the helix spanning residues 3–11. Theoretical analyses indicated that the helix is amphipathic and surface-seeking. CD and dynamic light scattering data evinced peptide and/or vesicle aggregation, modulated by peptide:lipid ratio and PG content. BP100 decreased the absolute value of the zeta potential (ζ) of LUVs with low PG contents; for higher PG, binding was analyzed as an ion-exchange process. At high salt, BP100-induced LUVS leakage requires higher peptide concentration, indicating that both electrostatic and hydrophobic interactions contribute to peptide binding. While a gradual release took place at low peptide:lipid ratios, instantaneous loss occurred at high ratios, suggesting vesicle disruption. Optical microscopy of GUVs confirmed BP100-promoted disruption of negatively charged membranes. The mechanism of action of BP100 is determined by both peptide:lipid ratio and negatively charged lipid content. While gradual release results from membrane perturbation by a small number of peptide molecules giving rise to changes in acyl chain packing, lipid clustering (leading to membrane defects), and/or membrane thinning, membrane disruption results from a sequence of events – large-scale peptide and lipid clustering, giving rise to peptide-lipid patches that eventually would leave the membrane in a carpet-like mechanism.
BP100 helical wheel (a), BP100-induced dense regions in PC:PG GUV (b). Proposed events at low or high BP100/lipid ratios and PG contents, leading to gradual or instantaneous leakage (c). Display omitted
•BP100 was calculated to be a surface-seeking amphipathic helix.•CD and NMR spectra showed that upon binding to PC:PG LUV, BP100 becomes α-helical.•Salt effects showed that BP100 binding had electrostatic and hydrophobic components.•BP100 mechanism of action depends on peptide:lipid ratio and surface charge.•At high BP100:lipid ratio leakage and GUV disruption suggest a carpet-like mechanism.
Microorganisms, usually bacteria and fungi, grow and spread in skin wounds, causing infections. These infections trigger the immune system and cause inflammation and tissue damage within the skin or ...wound, slowing down the healing process. The use of photodynamic therapy (PDT) to eradicate microorganisms has been regarded as a promising alternative to anti-infective therapies, such as those based on antibiotics, and more recently, is being considered for skin wound-healing, namely for infected wounds. Among the several molecules exploited as photosensitizers (PS), porphyrinoids exhibit suitable features for achieving those goals efficiently. The capability that these macrocycles display to generate reactive oxygen species (ROS) gives a significant contribution to the regenerative process. ROS are responsible for avoiding the development of infections by inactivating microorganisms such as bacteria but also by promoting cell proliferation through the activation of stem cells which regulates inflammatory factors and collagen remodeling. The PS can act solo or combined with several materials, such as polymers, hydrogels, nanotubes, or metal-organic frameworks (MOF), keeping both the microbial photoinactivation and healing/regenerative processes' effectiveness. This review highlights the developments on the combination of PDT approach and skin wound healing using natural and synthetic porphyrinoids, such as porphyrins, chlorins and phthalocyanines, as PS, as well as the prodrug 5-aminolevulinic acid (5-ALA), the natural precursor of protoporphyrin-IX (PP-IX).
The present study deals with the development of multifunctional biphasic calcium phosphate (BCP) scaffolds coated with biopolymers-poly(ε-caprolactone) (PCL) or poly(ester urea) (PEU)-loaded with an ...antibiotic drug, Rifampicin (RFP). The amounts of RFP incorporated into the PCL and PEU-coated scaffolds were 0.55 ± 0.04 and 0.45 ± 0.02 wt%, respectively. The in vitro drug release profiles in phosphate buffered saline over 6 days were characterized by a burst release within the first 8h, followed by a sustained release. The Korsmeyer-Peppas model showed that RFP release was controlled by polymer-specific non-Fickian diffusion. A faster burst release (67.33 ± 1.48%) was observed for the PCL-coated samples, in comparison to that measured (47.23 ± 0.31%) for the PEU-coated samples. The growth inhibitory activity against
and
was evaluated. Although the RFP-loaded scaffolds were effective in reducing bacterial growth for both strains, their effectiveness depends on the particular bacterial strain, as well as on the type of polymer coating, since it rules the drug release behavior. The low antibacterial activity demonstrated by the BCP-PEU-RFP scaffold against
could be a consequence of the lower amount of RFP that is released from this scaffold, when compared with BCP-PCL-RFP. In vitro studies showed excellent cytocompatibility, adherence, and proliferation of human mesenchymal stem cells on the BCP-PEU-RFP scaffold surface. The fabricated highly porous scaffolds that could act as an antibiotic delivery system have great potential for applications in bone regeneration and tissue engineering, while preventing bacterial infections.
Siderophores are small molecules of organic nature, released by bacteria to chelate iron from the surrounding environment and subsequently incorporate it into the cytoplasm. In addition to iron, ...these secondary metabolites can complex with a wide variety of metals, which is why they are commonly studied in the environment. Heavy metals can be very toxic when present in large amounts on the planet, affecting public health and all living organisms. The pollution caused by these toxic metals is increasing, and therefore it is urgent to find practical, sustainable, and economical solutions for remediation. One of the strategies is siderophore-assisted bioremediation, an innovative and advantageous alternative for various environmental applications.
This research highlights the various uses of siderophores and metallophores in the environment, underscoring their significance to ecosystems. The study delves into the utilization of siderophores and metallophores in both marine and terrestrial settings (e.g. bioremediation, biocontrol of pathogens, and plant growth promotion), such as bioremediation, biocontrol of pathogens, and plant growth promotion, providing context for the different instances outlined in the existing literature and highlighting their relevance in each field. The study delves into the structures and types of siderophores focusing on their singular characteristics for each application and methodologies used. Focusing on recent developments over the last two decades, the opportunities and challenges associated with siderophores and metallophores applications in the environment were mapped to arm researchers in the fight against environmental pollution.
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•Siderophores can chelate with several metals, forming soluble metallophores.•Siderophores reduce pollution caused by heavy metals.•Siderophores can enhance productivity in the aquaculture and agriculture sectors..•Siderophores have the ability to inhibit the iron supply to fish and plant pathogens.•Using siderophores to assist bioremediation is a promising approach to improve environmental health.
With the rising levels of drug resistance, developing efficient antimicrobial therapies has become a priority. A promising strategy is the conjugation of antibiotics with relevant moieties that can ...potentiate their activity by target-directing. The conjugation of siderophores with antibiotics allows them to act as Trojan horses by hijacking the microorganisms’ highly developed iron transport systems and using them to carry the antibiotic into the cell. Through the analysis of relevant examples of the past decade, this Perspective aims to reveal the potential of siderophore–antibiotic Trojan horses for the treatment of infections and the role of siderophores in diagnostic techniques. Other conjugated molecules will be the subject of discussion, namely those involving vitamin B12, carbohydrates, and amino acids, as well as conjugated compounds targeting protein degradation and β-lactamase activated prodrugs.
Antimicrobial resistance has become a major threat to public health worldwide, as pathogenic microorganisms are finding ways to evade all known antimicrobials. Therefore, the demand for new and ...effective antimicrobial agents is also increasing. Natural products have always played an important role in drug discovery, either by themselves or as inspiration for synthetic compounds. The marine environment is a rich source of bioactive metabolites, and among them, tryptophan-derived alkaloids stand out for their abundance and by displaying a variety of biological activities, with antimicrobial properties being among the most significant.
This review aims to reveal the potential of marine alkaloids derived from tryptophan as antimicrobial agents. Relevant examples of these compounds and their synthetic analogues reported in the last decades are presented and discussed in detail, with their mechanism of action and synthetic approaches whenever relevant.
Several tryptophan-derived marine alkaloids have shown potent and promising antimicrobial activities, whether against bacteria, fungi, or virus. Synthetic approaches to many of the compounds have been developed and recent methodologies are proving to be efficient. Even though most of the studies regarding the antimicrobial activity are still preliminary, this class of compounds has proven to be worth of further investigation and may provide useful lead compounds for the development of antimicrobial agents. Overall, marine alkaloids derived from tryptophan are revealed as a valuable class of antimicrobials and molecular modifications in order to reduce the toxicity of these compounds and additional studies regarding their mechanism of action are interesting topics to explore in the future.
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•The potential of marine alkaloids derived from tryptophan as antimicrobial agents is reviewed.•The most promising classes include aplysinopsins, brevianamides topsentins, hamacanthins, and eudistomins.•Synthetic approaches allowed development of analogues and SAR studies, with the introduction of halogens being a relevant feature to be included in new synthetic analogues to improve activity.•Antibacterial properties were the most relevant but promising antifungal and antiviral representatives are also reported.•Putative targets include MRSA pyruvate kinase and Mycobacterium tuberculosis shikimate kinase (MtSK).
Antioxidants have long been used in the cosmetic industry to prevent skin photoaging, which is mediated by oxidative stress, making the search for new antioxidant compounds highly desirable in this ...field. Naturally occurring xanthones are polyphenolic compounds that can be found in microorganisms, fungi, lichens, and some higher plants. This class of polyphenols has a privileged scaffold that grants them several biological activities. We have previously identified simple oxygenated xanthones as promising antioxidants and disclosed as hit, 1,2-dihydroxyxanthone (
). Herein, we synthesized and studied the potential of xanthones with different polyoxygenated patterns as skin antiphotoaging ingredients. In the DPPH antioxidant assay, two newly synthesized derivatives showed IC
values in the same range as ascorbic acid. The synthesized xanthones were discovered to be excellent tyrosinase inhibitors and weak to moderate collagenase and elastase inhibitors but no activity was revealed against hyaluronidase. Their metal-chelating effect (FeCl
and CuCl
) as well as their stability at different pH values were characterized to understand their potential to be used as future cosmetic active agents. Among the synthesized polyoxygenated xanthones, 1,2-dihydroxyxanthone (
) was reinforced as the most promising, exhibiting a dual ability to protect the skin against UV damage by combining antioxidant/metal-chelating properties with UV-filter capacity and revealed to be more stable in the pH range that is close to the pH of the skin. Lastly, the phototoxicity of 1,2-dihydroxyxanthone (
) was evaluated in a human keratinocyte cell line and no phototoxicity was observed in the concentration range tested.
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