Terahertz (THz) time-domain spectroscopy has been investigated for assessment of the hydration levels in the cornea, intraocular pressure, and changes in corneal topography. Previous efforts at THz ...imaging of the cornea have employed off-axis parabolic mirrors to achieve normal incidence along the spherical surface. However, this comes at the cost of an asymmetric field-of-view (FOV) and a long scan time because it requires raster-scanning of the collimated beam across the large mirror diameter. This paper proposes a solution by designing a pair of aspheric lenses that can provide a larger symmetric spherical FOV (9.6 mm) and reduce the scan time by two orders of magnitude using a novel beam-steering approach. A hyperbolic-elliptical lens was designed and optimized to achieve normal incidence and phase-front matching between the focused THz beam and the target curvature. The lenses were machined from a slab of high-density polyethylene and characterized in comparison to ray-tracing simulations by imaging several targets of similar sizes to the cornea. Our experimental results showed excellent agreement in the increased symmetric FOV and confirmed the reduction in scan time to about 3-4 seconds. In the future, this lens design process can be extended for imaging the sclera of the eye and other curved biological surfaces, such as the nose and fingers.
Zinc oxide doped single wall carbon nanotubes (ZnO:CNT) are incorporated in PEDOT:PSS aqueous solution to serve as a hole transport buffer layer in the preparation of thin film organic solar cells ...(TFOSC). The solar cells were fabricated according to bulkheterojunction design whose photoactive layer is composed of poly (3 hexythiophene) (P3HT) and 6,6-phenyl-C61-butyric acid methyl ester (PCBM) blend. Significant improvement in harvesting photo-generated currents and low series resistances were observed which lead to higher power conversion efficiencies compared to the devices without ZnO:CNT. The optical properties and surface morphologies of ZnO:CNT/PEDOT:PSS hole transport layers are investigated and compared with the changes in the measured parameters of the solar cells. The power conversion efficiency of the devices increased by nearly 116%, 63% and 42% for ZnO:CNT loading at 2.5%, 5% and 10% by weight, respectively, from the devices that uses only PEDOT:PSS as a hole transport layer. Furthermore, a complete recovery of device performance was found by storing the device in warm nitrogen atmosphere.
•The PCE of TFOSC has improved by 150% due to the inclusion of ZnO:CNT in hole transport layer.•High concentration of ZnO:CNT in PEDOT:PSS was unfavorable to device performance.•A complete recovery of device performance was found by storing TFOSC in warm nitrogen atmosphere.•The optical transmission of the film ZnO:CNT/PEDOT:PSS improved in the UV region due ZnO:CNT.
In recent years, handheld and portable terahertz instruments have been in rapid development for various applications ranging from non-destructive testing to biomedical imaging and sensing. For ...instance, we have deployed our Portable Handheld Spectral Reflection (PHASR) Scanners for in vivo full-spectroscopic imaging of skin burns in large animal models in operating room settings. In this paper, we debut the polarimetric version of the PHASR Scanner, and describe a generalized calibration technique to map the spatial and spectral dependence of the Jones matrix of an imaging scanner across its field of view. Our design is based on placement of two orthogonal photoconductive antenna (PCA) detectors separated by a polarizing beam splitter in the PHASR Scanner housing. We show that as few as three independent measurements of a well-characterized polarimetric calibration target are sufficient to determine the polarization state of the incident beam at the sample location, as well as to extract the Jones propagation matrix from the sample location to the detectors. We have tested the accuracy of our scanner by validating polarimetric measurements obtained from a birefringent crystal rotated to various angles, as compared to the theoretically predicted response of the sample. This new version of our PHASR scanner can be used for high-speed imaging and investigation of heterogeneity of polarization-sensitive samples in the field.
The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing G bases. Here we report the ...evolution of three new SpCas9 variants that collectively recognize NRNH PAMs (where R is A or G and H is A, C or T) using phage-assisted non-continuous evolution, three new phage-assisted continuous evolution strategies for DNA binding and a secondary selection for DNA cleavage. The targeting capabilities of these evolved variants and SpCas9-NG were characterized in HEK293T cells using a library of 11,776 genomically integrated protospacer-sgRNA pairs containing all possible NNNN PAMs. The evolved variants mediated indel formation and base editing in human cells and enabled A•T-to-G•C base editing of a sickle cell anemia mutation using a previously inaccessible CACC PAM. These new evolved SpCas9 variants, together with previously reported variants, in principle enable targeting of most NR PAM sequences and substantially reduce the fraction of genomic sites that are inaccessible by Cas9-based methods.
Many promising biomedical applications have been proposed for terahertz (THz) spectroscopy and diagnostic imaging techniques. Polarimetric imaging systems are generally useful for enhancing imaging ...contrasts, yet the interplay between THz polarization changes and the random discrete structures in biological samples is not well understood. In this work, we performed Monte Carlo simulations of the propagation of polarized THz waves in skin and adipose tissues based on the Mie scattering from intrinsic structures, such as hair follicles or sweat glands. We show that the polarimetric contrasts are distinctly affected by concentration, size and dielectric properties of the scatterers, as well as the frequency and polarization of the incident THz waves. We describe the experimental requirements for observing and extracting these polarimetric signals due to the low energy and small angular spread of the back-scattered THz radiation. We analyzed the spatially integrated Mueller matrices of samples in the normal-incidence back-scattering geometry. We show that the frequency-dependent degree of polarization (DOP) can be used to infer the concentrations and dielectric contents of the scattering structures. Our modeling approach can be used to inform the design of the imaging modalities and the interpretation of the spectroscopic data in future terahertz biomedical imaging applications.
Speckle patterns observed in coherent optical imaging reflect important characteristic information of the scattering object. To capture speckle patterns, angular resolved or oblique illumination ...geometries are usually employed in combination with Rayleigh statistical models. We present a portable and handheld 2-channel polarization-sensitive imaging instrument to directly resolve terahertz (THz) speckle fields in a collocated telecentric back-scattering geometry. The polarization state of the THz light is measured using two orthogonal photoconductive antennas and can be presented in the form of the Stokes vectors of the THz beam upon interaction with the sample. We report on the validation of the method in surface scattering from gold-coated sandpapers, demonstrating a strong dependence of the polarization state on the surface roughness and the frequency of the broadband THz illumination. We also demonstrate non-Rayleigh first-order and second-order statistical parameters, such as degree of polarization uniformity (DOPU) and phase difference, for quantifying the randomness of polarization. This technique provides a fast method for broadband THz polarimetric measurement in the field and has the potential for detecting light depolarization in applications ranging from biomedical imaging to non-destructive testing.
•Inflammation is an important mediator of secondary neurological injury after TBI.•Activation of CB2R attenuates inflammation and reduces neurovascular injury after TBI.•CB2R agonist, GP1a polarized ...macrophages into anti-inflammatory (M2) phenotypes.•GP1a reduced edema, enhanced cerebral blood flow, and improved behavioral outcomes.•CB2R antagonist, AM630 worsened physiological and behavioral outcomes after TBI.
Inflammation is an important mediator of secondary neurological injury after traumatic brain injury (TBI). Endocannabinoids, endogenously produced arachidonate based lipids, have recently emerged as powerful anti-inflammatory compounds, yet the molecular and cellular mechanisms underlying these effects are poorly defined. Endocannabinoids are physiological ligands for two known cannabinoid receptors, CB1R and CB2R. In the present study, we hypothesized that selective activation of CB2R attenuates neuroinflammation and reduces neurovascular injury after TBI. Using a murine controlled cortical impact (CCI) model of TBI, we observed a dramatic upregulation of CB2R within infiltrating myeloid cells beginning at 72 h. Administration of the selective CB2R agonist, GP1a (1–5 mg/kg), attenuated pro-inflammatory M1 macrophage polarization, increased anti-inflammatory M2 polarization, reduced edema development, enhanced cerebral blood flow, and improved neurobehavioral outcomes after TBI. In contrast, the CB2R antagonist, AM630, worsened outcomes. Taken together, our findings support the development of selective CB2R agonists as a therapeutic strategy to improve TBI outcomes while avoiding the psychoactive effects of CB1R activation.
We report on the development and performance characterization of a telecentric terahertz spectroscopic scanner using an f- θ objective lens and a single gimballed scanning mirror for image formation. ...We derived a beam steering transform to compensate for the intercoupling of the gimballed mirror axes and the distortions caused by an imperfect scanning lens. We characterize the optical performance of the system in both the time and spatial domains, demonstrating a constant diffraction-limited imaging resolution over the entire field of view. Finally, given the large depth of focus of the objective lens, we demonstrate the broadband imaging capability at different depths using a Boehler star target. This imaging setup has the potential to be miniaturized into portable form factors for field-deployable scenarios.
Clinical studies have indicated the existence of an “obesity paradox” in patients with chronic heart failure (HF), that is, reduced mortality in patients who have elevated body mass index (BMI) ...scores compared with normal-weight reference groups. The aim of this study was to investigate the relation of BMI with all-cause and cardiovascular (CV) mortality and hospitalization in patients with chronic HF though a systematic review and meta-analysis of published research. PubMed, the Cumulative Index to Nursing and Allied Health Literature, Cochrane Central, Scopus, web of science and Embase were searched for studies reporting rates of total mortality, cardiac mortality, and risk for hospitalization in patients with HF in various BMI categories (<20 kg/m2 low, 20 to 24.9 kg/m2 normal reference, 25 to 29.9 kg/m2 overweight, 30 to 34.9 obese, and ≥35 kg/m2 severely obese). Event rates were compared using a forest plot of relative risk (RR) using a random-effects model assuming interstudy heterogeneity. Two study investigators independently reviewed the 124 reports retrieved and identified 6 for final analyses (n = 22,807). After a mean follow-up period of 2.85 years, the risk for adverse events was highest in patients with low BMIs: total mortality RR 1.27 (95% confidence interval CI 1.17 to 1.37), CV mortality RR 1.20 (95% CI 1.01 to 1.43), and hospitalization RR 1.19 (95% CI 1.09 to 1.30). Risk for CV mortality and hospitalization was lowest in overweight patients (RR 0.79, 95% CI 0.70 to 0.90, and RR 0.92, 95% CI 0.86 to 0.97, respectively). Increasing degree of obesity failed to achieve a statistically significant effect on CV mortality (RR 0.82, 95% CI 0.64 to 1.05, and RR 0.71, 95% CI 0.50 to 1.01, for obese and severely obese, respectively) and on hospitalization (RR 0.99, 95% CI 0.92 to 1.07, and RR 1.28, 95% CI 0.88 to 1.87, for obese and severely obese, respectively). In conclusion, risk for total mortality and CV mortality and hospitalization was highest in patients with chronic HF who were underweight as defined by low BMI, whereas risk for CV mortality and hospitalization was lowest in overweight subjects.
Telecentric beam scanning using f-θ lenses offers nearly uniform spot size, linear beam displacement, and normal incidence angle over a planar surface. These unique properties allow for the ...minimization of imaging distortion over a wide field-of-view. In this article, we present a numerical method for designing custom f-θ lenses in the THz regime. We fabricated three lenses made from different commonly used polymer materials in the THz optics. We demonstrated their optical performance metrics compared to a conventional plano-convex lens over the broadband 0.3 THz–1 THz range. We find that the f-θ lens designed using the optical properties of high-density polyethylene achieved superior performance by maintaining a constant phase over a wide field of view of about 34°. We demonstrate this isophase property by measuring a constant time of arrival of the THz time-domain pulses over a reference mirror with a standard deviation of ∼19 fs, in excellent agreement with simulation predictions. This work will pave the way for the design and implementation of highly precise and fast telecentric imaging systems in the THz frequencies.
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