•The green Ag NPs caused single strain DNA cleavage activity for 30 and 60 min at 50 and 100 mg/L, respectively.•The microwave assisted green synthesis of monodisperse Ag NPs by using Reishi ...Mushroom.•The highest DPPH scavenging percentage was recorded as 76.45% at 250 mg/L Ag NPs.•One of the highest effectiveness of Ag NPs for the inhibition of proliferation of gram-positive and gram-negative bacteria.
In recent years, the synthesis of nanoparticles via biological processes has attracted considerable attention. The use of plants and plant extracts is one of the most preferred methods for biological synthesis due to their rich biologically active metabolites. In this study, silver nanoparticles (Ag NPs) were synthesized using reishi mushroom (Ganoderma lucidum) extract. Different analytical techniques including X-ray Photoelectron Spectroscopy (XPS), X-ray diffraction (XRD), transmission electron microscopy (TEM), UV–vis spectroscopy, and Fourier Transform Infrared Spectrophotometer (FTIR) were used for the characterization of Ag NPs. UV–vis spectrum exhibited a broad absorption peak between 400–460 nm which indicates the existence of Ag NPs. TEM images showed Ag NPs are spherical with a diameter range of 15–22 nm. In addition, it is shown that Ag NPs form a face-centered cubic structure according to XRD characterization technique. The antioxidant activity towards to 1-Diphenyl-2-picrylhydrazyl (DPPH) was also studied. The highest DPPH scavenging percentage was recorded as 76.45% at 250 mg/L. The DNA cleavage activity results indicated that the green Ag NPs caused single strain DNA cleavage activity for 30 and 60 min at 50 and 100 mg/L, respectively. The Ag NPs antimicrobial activity was also investigated and results recorded as minimum inhibition concentration (MIC). Ag NPs showed a strong antibacterial effect against gram-positive (S. aureus, E. hirae, B. cereus) and gram-negative (E. coli, P. aeruginosa, L. pneumophila subsp. Pneumophila) bacteria. Furthermore, Ag NPs have also been shown to have a high antifungal effect against C. albicans fungus.
Biosynthesized platinum nanoparticles using pomegranate extract. (PtCl4: Platinum(IV) chloride).
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•The green synthesis of monodisperse Pt NPs in microwave conditions by using ...Pomegranate peel,.•The inhibition of proliferation of human breast cancer cell line in the IC50 at a dose of 17.84 μg/ml Pt NPs.•One of the highest effectiveness of Pt NPs for the inhibition of proliferation of human breast cancer cell line.•Its proliferative effect by reducing DNA synthesis and apoptosis-inducing cell cycle stages.
The study utilizes monodisperse platinum nanoparticles (Pt NPs) biosynthesized from Punica granatum crusts as anti-tumor agents on the human breast cancer cell line, MCF-7. The obtained Pt NPs were fully characterized using the UV–vis spectrum (UV–vis), transmission electron microscopy (TEM), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and Fourier transformation infrared spectroscopy (FTIR). Effectiveness of the Pt NPs was determined by cell viability, propidium iodide staining test, flow cytometry and comet tests on the MCF-7 cancer cell line. Cell survival percentage was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The biosynthesized monodisperse platinum nanoparticles inhibited MCF-7 proliferation with an IC50 of 17.84 μg/ml after 48 h of incubation. Propidium iodide staining demonstrated that the monodisperse Pt NPs induced apoptosis by means of molecular DNA fragmentation.
Addressed herein, a highly efficient, durable and uniformly dispersed activated carbon supported palladium–iridium nanomaterials (3.42 ± 0.34 nm) were reported for the first time as a catalyst in ...dimethylamine-borane dehydrogenation reaction at the room temperature. The activated carbon supported palladium-iridium nanosheet (Pd–Ir NPs) is obtained by a simple ultrasonic reduction method, and the fabricated nanocatalyst have been defined by Ultra-Violet-Visible (UV–VIS), Raman spectroscopy, X-Ray Diffraction (XRD), X-Ray Photoelectron Spectroscopy (XPS), Transmission Electron Microscopy (TEM) and High-Resolution Transmission Electron Microscopy (HR-TEM). These newly prepared Pd–Ir nanocomposites were found to be highly efficient and stable for dehydrocoupling of dimethylamine borane. The catalytic activity of the Pd–Ir NPs was excellent by showing the one of the best catalytic activity with a very high turnover frequency (295.1 h−1) and low Ea value of 36.6 ± 2 kJ/mol for DMAB dehydrocoupling. Another important fact about the prepared catalyst is the reusability of the catalyst was very high and easily reused five times without any significant decrease in their catalytic performance. In the current work, the synthesize, characterization and the catalytic performance of the Pd–Ir nanoparticles for the dehydrogenation of the DMAB reaction will be discussed in detail.
•The synthesis and characterization of PdIr@AC NPs as highly efficient catalysts.•PdIr@AC NPs were used for dehydrocoupling of DMAB as heterogeneous catalysts.•The prepared PdIr@AC NPs provides one of the best catalytic performances.•Detailed kinetic study of the catalytic dehydrogenation of dimethylamine-borane were examined.
Highly effective and stable palladium nanoparticles (Pd NPs) supported on graphene oxide (GO) were synthesized, characterized and applied for dehydrogenation of dimethylamine-borane (DMAB). ...Monodisperse Pd NPs were synthesized with the ultrasonic reduction method in the presence of oleylamine and GO as support matrices at room temperature. Almost uniformly distributed Pd (0) nanoparticles in size of 3.89 nm were isolated in a reproducible manner by filtering through the reaction mixture. They were characterized by TEM, HRTEM, XRD, Raman, and XPS. It was found that Pd@GO NPs are stable and one of the most active catalysts in the dehydrogenation of DMAB. This catalyst with its turnover frequency of 38.02 h−1 exhibits one of the good results among all the catalysts prepared in the literature for dehydrogeneration of DMAB. Significant activation parameters of the catalytic dehydrogenation reaction were also calculated, and the activation enthalpy (ΔH#), activation entropy (ΔS#) and the apparent activation energy (Ea) were found 16.5 kJ mol−1, −179 J mol−1 K−1, and 18.6 kJ mol−1, respectively.
•The discovery of an efficient dimethylamine–borane dehydrogenation catalyst.•Monodisperse Palladium Nanoparticles stabilized by Graphene oxide (Pd@GO) nanoclusters.•The one of the good TOF value of Pd@GO NPs for dimethylamine–borane dehydrogenation.•Thanks to the small sizes, monodispersity and high Pd (0) % surface of novel catalyst.
This study focuses on the use of green synthesized silver nanoparticles (Ag-NPs) with the aid of Camellia sinensis (black tea) extract to provide antibacterial activity on ceramic structure. The ...synthesized Ag nanoparticles were added to the glaze used in the ceramic structures and mixed homogeneously. The homogeneous mixture was characterized by transmission electron microscopy (TEM), X-Ray Diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and UV–Vis spectroscopy (UV–Vis), Fourier-transform infrared spectroscopy (FTIR) techniques. The SPR band of the synthesized biogenic Ag NPs was observed as 422 nm during the reaction at room temperature. TEM analysis revealed that Ag NPs were spherical and a particle size between 10 and 20 nm. Furthermore, the antibacterial properties of the homogeneous mixture (Ag NPs and glaze) were tested against Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Methicillin-resistant Staphylococcus aureus (MRSA) bacteria. Biogenic Ag NPs at a concentration of 100 μg/ml were observed to have 90%, 75%, 75%, 80% lethal effects against S. aureus, MRSA, B. Subtilis, and E. Coli bacteria, respectively. The antibacterial results of Ag NPs obtained with the help of Camellia sinensis show that they may have potential application and development in the field of ceramics. In addition, the antibacterial activity of commercially available antibiotics and the prepared Ag NPs were analyzed in ceramic glazes.
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•The green synthesis of monodisperse Ag NPs by using Camellia sinensis (Black Tea).•One of the highest effectiveness of Ag NPs for the inhibition of proliferation of gram-positive and gram-negative bacteria.•Ag nanoparticles added to the ceramic glaze are effective in preventing bacterial growth.•Ag-doped samples show better antibacterial effect.
•The microwave assisted green synthesis of monodisperse AgNPs by using Pomegranate peel.•The inhibition of proliferation of human breast cancer cell line in the IC50 at a dose of 12.85μg/mL ...AgNPs.•One of the highest effectiveness of AgNPs for the inhibition of proliferation of human breast cancer cell line.•Its proliferative effect by reducing DNA synthesis and apoptosis-inducing cell cycle stages.
In this study, we aimed to investigate whether the combination therapy of pomegranate extract and silver nanoparticle is effective on MCF-7 cell culture. The pomegranate extract was mixed and incubated with silver nitrate for the microwave assisted green synthesized of silver nanoparticle. Obtained nanoparticles were investigated using X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), UV–vis, Field Emission Scanning Electron Microscopy (FESEM), and Transmission Electron Microscopy (TEM) methods The spectroscopic and morphological studies of the monodisperse Ag NPs which have particle size of 15.4nm indicate the highly crystalline form, well dispersity, and colloidally stable NPs. After fully characterization of prepared nanoparticles, the effectiveness of Ag NPs was determined by evaluating cell viability, nuclear degradation and cell cycle parameters. The results obtained demonstrate that biosynthesized Ag NPs can inhibit the proliferation of human breast cancer cell line MCF-7 in the IC50 at a dose of 12.85μg/mL and inhibit the proliferation of Ag NPs against anti-growth arresting MCF-7 cell line. This case demonstrates that it may exert its proliferative effect by reducing DNA synthesis and apoptosis-inducing cell cycle stages.
Green synthesis is considered to be one of the most suitable method because it enhances the therapeutic effects of palladium nanoparticles (Pd NPs). In this study, various biological activities such ...as antimicrobial, anticancer, antioxidant, and DNA cleavage activities of Urtica‐mediated green synthesizing Pd NPs were investigated. The synthesized Pd NPs were characterized by using UV‐vis, XPS, FT‐IR, TEM, and XRD analyses. As a result of the TEM analysis of Pd NPs, the mean particle size was found to be 7.44 ± 1.94 nm, and this result was supported by XRD analysis. The maximum DPPH scavenging activity was determined as 79.6% at 500 mg/L. The newly green synthesized Pd NPs exhibited high antimicrobial activity to gram‐negative bacteria than gram‐positive bacteria. Urtica‐mediated green synthesized Pd NPs also showed double strain DNA cleavage activity. For the cytotoxic effects of Pd NPs, the MDA‐MB‐231 breast cancer cell line, HT‐29 colon cancer cell line, Mia Paca‐2 human pancreatic cancer cell line, and healthy cell line L929‐Murine fibroblast cell line were used. IC50 values of Pd NPs against MDA‐MB‐231, HT‐29, and MIA PaCa‐2 cancer cell lines were calculated as 31.175, 20.383, and 29.335 μg/ml, respectively. No significant cytotoxic effect was observed in the healthy lines L929.
Various biological activities such as antimicrobial, anticancer, antioxidant, and DNA cleavage activities of Urtica‐mediated green‐synthesizing Pd NPs were investigated. Urtica‐mediated green‐synthesizing Pd NPs were characterized using UV‐vis, FT‐IR, XPS, TEM, and XRD analysis.
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This study reports on one of the best heterogeneous catalysts for the dehydrogenation of dimethylamine–borane (DMAB). This new catalytic system consists of highly monodisperse Pd and ...Ru alloy nanoparticles supported by poly(N-vinyl-pyrrolidone) (PdRu@PVP). The prepared heterogeneous catalyst can be reproducibly formed using an ultrasonic reduction technique for DMAB dehydrogenation under mild conditions. For the characterization of PdRu@PVP nanomaterials, several spectroscopic and microscopic techniques were used. The prepared PdRu@PVP nanomaterials with an average particle size of 3.82 ± 1.10 nm provided an 808.03 h−1 turnover frequency (TOF) in the dehydrogenation of DMAB and yielded 100% of the cyclic product (Me2NBH2)2 under mild conditions. Furthermore, the activities of catalysts were investigated theoretically using DFT-B3LYP calculations. The theoretical results based on density functional theory were in favorable agreement with the experimental data.
In this study, it was hypothesis that A. mongoliensis could be used as bioindicator for Ni (II) and Co (II). Thus, Ni (II) and Co (II) resistance, removal, bioaccumulation, and the impacts of them on ...antioxidant enzyme systems of thermophilic Anoxybacillus mongoliensis were investigated in details. The bioaccumulation of Ni (II) and Co (II) on the cell membrane of thermophilic A. mongoliensis, variations on surface macrostructure and functionality by FT-IR and SEM, and determination of antioxidant enzyme activities were also tested. The highest bioaccumulation values of Co (II) and Ni (II) were detected as 102.0 mg metal/g of dry bacteria at 10 mg/L for the 12th h and 90.4 mg metal/g of dry bacteria for the 24th h, respectively, and the highest Ni (II) and Co (II) cell membrane bioaccumulation capacities of A. mongoliensis were determined as 268.5 and 274.9 mg metal/g wet membrane, respectively at the 24th h. In addition, increasing on SOD and CAT activities were observed on depend of concentration of Ni (II) and Co (II) with respect to control. The antioxidant enzyme activity results also indicated that A. mongoliensis might be used as a bioindicator for Ni (II) and Co (II) pollution in environmental water specimens.
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•Bioaccumulation of Ni (II) and Co (II) metals on the antioxidant enzymes of thermophilic Anoxybacillus mongoliensis bacteria•Activity testing of SOD and CAT enzymes at different Ni (II) and Co (II) concentrations•Usability of thermophilic Anoxybacillus mongoliensis bacteria for recovery of toxic metals in industrial wastewater
