Background: An accurate histopathological assessment and reporting of testicular biopsies require an appropriate tissue fixative. We assessed the histological, histochemical, and immunohistochemical ...quality of testicular biopsies, comparing 10% formalin versus Bouin solution as tissue fixatives. Methods: This experimental study utilized 20 adult male albino rats equally divided into five cages for 30 days. By the end of the experiment, all animals were anesthetized, and both testes were removed and weighted; one testicle was fixed in 10% formalin and the other testicle in Bouin solution, offering 40 specimens and then subjected to histological, morphometric, histochemical, and immunohistochemical assessments. Results: Formalin revealed high-quality cytological details and better nuclear chromatin detail (P=0.03). At the architectural level, the Bouin solution showed better quality details with less cytoplasmic shrinkage of seminiferous tubule germ cells (P=0.001). Bouin’s fixed tissues were more suitable for staining by trichrome methods but unsuitable when subsequent immunohistochemistry was requested. The diagnostic concordance between the Bouin solution versus formalin-fixed biopsies was 91.7%. Conclusion: This study supports that the morphology of testicular tissue fixed with Bouin solution was nearly comparable to those fixed with 10% neutral buffered formalin. However, the Bouin solution cannot substitute formalin when subsequent immunohistochemistry is considered.
Objectives: To assess the diagnostic accuracy of haematoxylin-eosin staining in clinically suspected Hirschsprung disease, and to compare the findings with calretinin and S100 immunohistochemistry.
...Method: The retrospective study was conducted at the AL-Khansaa Teaching Hospital, Nineveh, Iraq, and comprised data from January 2017 to October 2020 of rectal suction biopsies of patients with clinically and radiologically suspected Hirschsprung disease. Histopathology and immunohistochemistry were performed. Data was analysed using SPSS 16.
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Background: Helicobacter species pylori represent one of the medically prominent and most common infections in the world. Contamination with this microbe has set as a causal factor in the development ...of gastritis, peptic ulcer, and gastric neoplasia. Consequently, prompt diagnosis is essential. Objectives: This study was conveyed to detect H.pylori in gastric biopsies specimens by using routine Hematoxylin, Modified Giemsa dye as well as immunohistochemical stain, besides to assess the specificity and sensitivity of Helicobacter microbe detection in each method. Patients and methods:The research was both prospective and retrospective, carried out on 100 cases of endoscopically obtained gastric biopsies. Data obtained from archives of the pathology department, at AL-Jamhuri Teaching Hospital, Mosul city, and collected in a period spanning from April 2013 to March 2014. The information included; Age, sex, gastric biopsy location, inflammation status, the presence of dysplasia or carcinoma. Helicobacter pylori infection was assessed histochemically and immunohistochemically. Results: In a total of 100 gastric samples, patients’ age range was 11 to 82 years (mean age of 46.5 years), with a male to female ratio of 1.38:1. Helicobacter pylori bacilli were positive with H&E/MGS in 71 (71%) of cases, increased to 75 (75%) case with IHC. Chronic gastritis noticed in 85 biopsy specimens, 74% were positive for H.pylori. There was a statistically significant difference between IHC and H&E/MGS (p=0.04) for detection of H.pylori. The sensibility and specificity of the H&E/MGS were measured compared with the recommended standard sensitive and specific IHC test; they were 95% and 100% respectively. Conclusion: The routine ancillary stains request for the detection of H.pylori remains a laboratory and an institution right. This study revealed that, in our laboratory, the regular application of ancillary dyes is not obliged for the description of H.pylori because it was readily recognizable in the bulk of sections with haematoxylin staining. However, we recommend the use of IHC in specific circumstances.
Objectives: Programmed Cell Death Ligand1 (PD-L1) tissue expression in CRC (colorectal cancer) displays conflicting results among various studies. We aimed to identify the rate of PD-L1 positivity in ...colorectal carcinoma, and it's immune infiltrating cells, their relationship with clinicopathologic parameters of patients, and to correlate the results with other studies.
Methods: PD-L1 antibody retrospectively analyzed immunohistochemically in tissue microarray blocks of 99 specimens with colonic and rectal carcinomas operated between January 2015 to December 2017. A comparison performed between PD-L1 expression in tumor cells (TCs) as well as tumor-infiltrating immune cells (TIICs) for age, sex, histological differentiation, the primary tumor location, number of involved lymph nodes, angiolymphatic invasion, and TNM stage.
Results: Of the 99 patients, the median age was 54.5 (range: 18 to 83) years. Fourteen samples were PD-L1 positive in TCs, increased to 32% in TIICs. A significant expression of PD-L1in TCs was correlated with medullary histology (p= 0.03), number of the involved lymph nodes (p= 0.02), distant metastasis (p= 0.001), and TNM stage (p= 0.0001). The PD-L1 status in TIICs was again connected with adverse clinical and pathological parameters.
Conclusions: The expression of PD-L1 in TCs and TIICs is associated significantly with advanced cancer or lymphatic invasion in patients who underwent surgery after a diagnosis of CRC. The research designates the significance of estimation of TCs and TIICs in correlation to clinicopathologic characteristics of patients a finding that could produce a piece of evidence for precise electing immunotherapy.
OBJECTIVETo assess the diagnostic accuracy of haematoxylin-eosin staining in clinically suspected Hirschsprung disease, and to compare the findings with calretinin and S100 immunohistochemistry. ...METHODSThe retrospective study was conducted at the AL-Khansaa Teaching Hospital, Nineveh, Iraq, and comprised data from January 2017 to October 2020 of rectal suction biopsies of patients with clinically and radiologically suspected Hirschsprung disease. Histopathology and immunohistochemistry were performed. Data was analysed using SPSS 16. RESULTSOf the 114 patients, 74(64.9%) were males and 40(35.1%) were females. Based on histology, 28(24.6%) cases were negative for ganglion cells, and, of them 25(89.2%) revealed nerve bundle hypertrophy. The diagnostic accuracy for the detection of ganglion cell and nerve hypertrophy using haematoxylin-eosin stain was 99.1% and 94.4%, respectively. Correlation of haematoxylin-eosin staining with calretinin and S100 was statistically near perfection (κ= 0.976 and κ = 0.923), respectively. CONCLUSIONSThe mainstay to confirm or exclude Hirschsprung disease remains an accurate histopathological evaluation of the haematoxylin-eosin-stained sections of an adequate colorectal biopsy.
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