Amaç: Bu araştırmada, palyatif bakım ünitesinde görev yapan hemşirelerin ölüm sürecindeki bakımında müdahale ve bakımlarını etkileyen faktörler değerlendirildi. Gereç ve Yöntem: Araştırmanın çalışma ...grubunu bir eğitim ve araştırma hastanesinin palyatif bakım ünitesinde görev yapan ve araştırmaya katılmayı kabul eden 22 hemşire oluşturdu. Araştırma, Mart 2017- Ağustos 2017 tarihleri arasında yapıldı ve nitel fenomenolojik yöntem kullanıldı. Veriler araştırmacılar tarafından hazırlanan “Bilgi Formu” ve “Yarı Yapılandırılmış Soru Formu” ile toplandı. Verilerin değerlendirilmesinde içerik analizi yöntemi kullanıldı. Bulgular: Araştırmaya katılan hemşirelerin yaş ortalaması 33.05±7.20 olup, %77.3’ü kadındı. Palyatif bakım ünitesinde çalışma süreleri (ay) 37.2±18.8’di. Üç tema elde edildi. Bunlar “ölüme bakış açısının değişmesi”, “ölüm anındaki müdahale ve bakım sürecinde hemşirelere hasta yakınları tarafından çıkarılan zorluklar” ve “palyatif bakım ünitesindeki mevcut yapılandırmada yaşanılan sorunlar”. Sonuç: Palyatif bakım ünitesinde çalışan hemşirelerin ölüm sürecine ilişkin görüşlerinin değiştiği ve müdahalelerde değişen koşulların sorunlar oluşturduğu tespit edildi.
It is well known that free oxygen radicals play an important role in the pathogenesis of several chronic disorders. Antioxidants are known as potential scavengers of reactive oxygen species that can ...protect biologic membranes against oxidative damage. Recent interest in phytochemicals has increased because of their protective effects against free oxygen radicals. Lycopene, which belongs to the carotenoid family, is the most effective singlet oxygen scavenger
of all the carotenoids. Foods that contain lycopene and related supplements have been reported to prevent chronic diseases including cancer, asthma, and cardiovascular disorders. The aim of the article was to give a brief review of the antioxidant properties and beneficial health effects of lycopene.
Amaç: İş sağlığı hemşireliği, hemşireliğin çalışma alanlarından biridir. İş sağlığı ve güvenliği uygulamalarının önemi gün geçtikçe artmakta ve öğrencilerin iş sağlığı alanında çalışma konusunda ...görüşleri önem kazanmaktadır. Bu çalışmada öğrencilerin iş sağlığı hemşireliği alanında çalışma konusundaki düşüncelerini ve görevleri konusundaki bilgi düzeylerini öğrenmek amaçlanmıştır.Yöntem: Çalışma, 2019 yılı Haziran ayında 210 hemşirelik öğrencisiyle yürütülen kesitsel bir çalışmadır. Veriler, araştırmacılar tarafından soru formuyla sınıfta gözlem altında toplandı. Verilerin analizinde yüzde, ortalama, standart sapma ve kikare testi kullanıldı. Anlamlılık değeri p lt;0,05 olarak kabul edildi.Bulgular: Öğrencilerin %67’si kız, yaş ortalamaları 21,5±1,49’dur. Mezun olduklarında %55,6’sı hastanede, 29,5’i ASM/TSM’de çalışmak istemektedir. İş sağlığı alanında %17,6’sı çalışmaktan memnun olacağını belirtti. Öğrenciler iş sağlığı alanında çalışan bir hemşirenin %77,6 oranıyla en fazla eğitici rolünü kullanılacağını ve %41,4 oranıyla çalışma ortamıyla ilgili sorunları saptama görevi olduğunu ifade etti. Hemşirelikle ilgili mevzuat içerisinde işyeri hemşireliğinin tanımlandığı yasa-yönetmelik-yönerge olduğunu belirten %42,4 olup 4. sınıflarda ve iş sağlığı ile ilgili ders alanlarda anlamlı fazla bulundu.Sonuç: Öğrencilerin yarısı mezun olduklarında hastanelerde çalışmak isterken, dörtte üçü işyerinde de çalışabileceğini belirtti. İş sağlığıyla ilgili derslerin, bilgi düzeylerini artırdığı, ancak çalışma isteğini etkilemediği görüldü.Anahtar kelimeler: Hemşirelik, iş sağlığı, öğrenci
Background and Aims: Our study aimed to evaluate how curcumin affect cisplatin cytotoxicity in human cervical carcinoma (HeLa), human hepatocellular carcinoma (HepG2), and Chinese hamster lung ...fibroblast (V79) cells. Methods: The cytotoxicity was evaluated by MTT assay. Results: The IC.sub.50 values of curcumin were 404 microM and 320 microM in HeLa cells; 236 microM and 98.3 microM in HepG2 cells; 877 microM and 119 microM in V79 cells; for 24 h and 48 h, respectively. The IC.sub.50 values of cisplatin were 22.4 microM and 12.3 microM in HeLa cells; 25.5 microM and 7.7 microM in HepG2 cells; 15.4 microM and 4.9 microM in V79 cells; for 24 h and 48 h, respectively. Curcumin significantly decreased cisplatin cytotoxicity at 500 microM in HeLa cells and above 250 microM and 125 microM in HepG2 cells, for 24 h and 48 h, respectively. In V79 cells, curcumin significantly decreased the IC.sub.50 values of cisplatin above 500 microM and 125 microM for 24 hand 48 h. Conclusion: The results might contribute to the anticancer effect of the curcumin-cisplatin combination in cervical and hepatocellular carcinoma, but in order to support this result and determine its interactions with antineoplastic drugs, further studies are needed. Keywords: Curcumin, cisplatin, cytotoxicity, HeLa cells, HepG2 cells
The increases of free radicals have been proposed to be involved in the pathogenesis of sepsis, which leads to multiple-organ dysfunction syndromes. The uses of antioxidants as a complementary tool ...in the medical care of oxidative stress-related diseases have attracted attention of researchers. Resveratrol (RV) has suggested being antioxidant, anti-proliferative, and anti-inflammatory effects in various experimental models and clinical settings.
This study was undertaken to evaluate the protective effects of RV on oxidative DNA damage induced by sepsis in the liver and kidney tissues of Wistar albino rats.
Animal experimentation.
Four experimental groups consisting of eight animals for each was created using a total of thirty-two male Wistar albino rats. Sham group was given 0.5 mL of saline intra-peritoneal (ip) only following laparatomy. Sepsis group was given 0.5 mL saline ip only following the induction of sepsis. RV-treated group was given a dose of 100 mg/kg ip RV in 0.5 mL saline following laparatomy. RV-treated sepsis group was given 100 mg/kg ip RV in 0.5 mL saline following the induction of sepsis. A model of sepsis was created by cecal ligation and puncture technique. In the liver and kidney tissues, oxidative stress parameters (malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPX)) and a proinflammatory cytokine (tumor necrosis factor alpha (TNF-alpha)), were evaluated spectrophotometrically and DNA damage was determined by the alkaline single cell gel electrophoresis (comet assay) technique using formamidopyrimidine DNA glycosylase protein.
In the RV-treated sepsis group, the levels of MDA and TNF-alpha were lower and GSH levels, SOD and GPX activities were higher than in the septic rats (p<0.05). RV treatment significantly reduced the sepsis-induced oxidative DNA damage in the liver and kidney cells (p<0.05).
It is suggested that RV treatment might reduce the sepsis-induced oxidative DNA damages in sepsis-related diseases; however, there is a need for more studies to clear up the protective mechanisms of RV against sepsis.
Phenolic compounds exhibit several health protective properties. Galangin, curcumin, pycnogenol, puerarin and ursolic acid are commonly used plant phenolics in folk medicine. The aim of our study was ...to evaluate the difference between neutral red uptake (NRU) and MTT assays using different plant phenolics (galangin, curcumin, pycnogenol, puerarin and ursolic acid) in healthy and cancer cells in different time periods.
In this study, the cytotoxic effects of these phenolic compounds were investigated by NRU and MTT assays in healthy (V79, Chinese hamster fibroblast cell line) and cancer human cervix epithelial adenocarcinoma cell line Henrietta Lacks (HeLa) and human mammary carcinoma cell line (BT-474) in 18, 24 and 48 h incubation periods.
Our results demonstrated that galangin, curcumin, pycnogenol, puerarin and ursolic acid decreased cell viability of V79, HeLa and BT-474 cells in a dose-dependent manner in 18, 24 and 48 h incubation periods. However, the cell survival rate was much lower in 48 h incubation period. There was no difference between the results from NRU and MTT assays.
To decide which incubation period and which cytotoxicity study to be used, the cytotoxicity mechanism of the compound must be known.
Silver sulfide (Ag
S) quantum dots (QDs) are highly promising nanomaterials in bioimaging systems due to their high activities for both imaging and drug/gene delivery. There is insufficient research ...on the toxicity of Ag
S QDs coated with meso-2,3-dimercaptosuccinic acid (DMSA). In this study, we aimed to determine the cytotoxicity of Ag
S QDs coated with DMSA in Chinese hamster lung fibroblast (V79) cells over a wide range of concentrations (5-2000 μg/mL).
Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assays. The genotoxic and apoptotic effects of DMSA/Ag
S QDs were also assessed by comet assay and real-time polymerase chain reaction technique, respectively.
Cell viability was 54.0±4.8% and 65.7±4.1% at the highest dose (2000 μg/mL) of Ag
S QDs using the MTT and NRU assays, respectively. Although cell viability decreased above 400 μg/mL (MTT assay) and 800 μg/mL (NRU assay), DNA damage was not induced by DMSA/Ag
S QDs at the studied concentrations. The mRNA expression levels of
, and
genes were altered in the cells exposed to 500 and 1000 μg/mL DMSA/Ag
S QDs.
The cytotoxic effects of DMSA/Ag
S QDs may occur at high doses through the apoptotic pathways. However, DMSA/Ag
S QDs appear to be biocompatible at low doses, making them well suited for cell labeling applications.
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