Actinomycetes are filamentous, Gram-positive bacteria widely distributed in soil and can produce secondary metabolites useful as antibiotics. The synthesis of secondary metabolites of Actinomycetes ...is influenced by the growth conditions of each isolate, including the fermentation period. Klebsiella pneumoniae is an opportunistic bacterial pathogen causing lower respiratory tract infection. Objectives: To determined the inhibition activity of Actinomycetes with different fermentation periods against the growth of K.pneumoniae. Methods: Actinomycetes were isolated from soil in Kebun Raya Bogor and grown on Starch Casein Agar (SCA) medium before being fermented in liquid media consisting of 2% of mannitol, 2% of peptone, and 1% of glucose with an incubation time of 6,7, and 8 days. An antibacterial activity test was conducted on Mueller Hinton Agar (MHA) medium using the well diffusion method. Results: The activity of Actinomycetes isolates with fermentation duration of 6,7 and 8 were able to inhibit the growth of K. pneumoniae with an average inhibition zone of 4,46 mm; 4,94 mm; and 5,04 mm, respectively. Actinomycetes' inhibitory mechanism ranges from inhibition of cell wall synthesis to nucleic acid and protein synthesis. One Way ANOVA test showed significant differences between the inhibition zone produced by each fermentation group. Conclusion: Actinomycetes isolates have antibacterial activity against the growth of K. pneumoniae with the highest activity achieved by Actinomycetes with a fermentation time of 8 days.Keywords: Actinomycetes, antibacterial, fermentation, Klebsiella pneumoniae
Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that ...produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa
Covid-19 outbreak that originated in Wuhan's city is spreading very quickly and is currently a pandemic worldwide. Transmission through droplets that come out of the patient's nose or mouth, whether ...symptomatic or asymptomatic. Efforts to prevent and fight COVID-19 must be carried out jointly with the community's cooperation and require strong discipline by washing hands frequently, maintaining distance, wearing masks, staying at home, and increasing body power. The purpose of this study was to determine the level of cadre compliance in carrying out health protocols in the era of the Covid-19 pandemic. This research method with a cross-sectional approach. The respondents were cadres of Limo Health Center, Depok. It was total sampling. The research instrument used a questionnaire of Google Form and analyzed using Chi-Square. The results showed that most of the respondents were included in the 46-60 year interval (51.4%) were highly educated, tertiary education (70%), knowledgeable (54.3%), unemployed (87.1), obeyed the protocol. Health (50%). Compliance increases in the elderly, higher education, sufficient knowledge, work status. conclusion there is no relationship between age (p = 0.632), education level (p = 0.794), knowledge level (p = 0.253), work status (p = 0.074) and compliance with the Covid-19 health protocol.
Acne vulgaris adalah suatu kondisi inflamasi umum pada unit polisebaseus, ditandai dengan komedo, papul, pustul atau nodul. Penyakit kulit ini bukan merupakan penyakit yang berbahaya tetapi mempunyai ...dampak yang besar secara fisik maupun psikologik. Prinsip penanganan acne antara lain menurunkan populasi Propionibacterium acne dan menekan inflamasi. Dari penelitian sebelumnya ditemukan adanya mikroorganisme lainnya dalam lesi yang mungkin berperan selain Propionibacterium acne yaitu Staphylococcus aureus, S. epidermidis dan Pityrosporum ovale. Beberapa sumber melaporkan ada beberapa manfaat Aloe vera untuk kecantikan dan perawatan kulit. Aloe vera juga digunakan secara eksternal untuk mengobati berbagai kondisi kulit seperti luka, nyeri dan menekan proses inflamasi. Penelitian ini dilakukan untuk mengetahui efek antimikroba ekstrak Aloe vera konsentrasi 25%,50% dan 75% terhadap isolat bakteri penyebab Acne vulgaris secara invitro dengan menggunakan metode difusi. Sebelum pengujian dilakukan isolasi dan identifikasi bakteri dari lesi. Bakteri yang ditemukan dari lesi penderita tergolong bakteri golongan Gram positif yaitu Staphylococcus aureus dan Propionibacterium acne. Hasil uji One-way Anova menunjukan terdapat perbedaan bermakna efektivitas ekstrak lidah buaya terhadap S.aureus dan P.acne. Uji Post Hoc terhadap S.aureus menunjukan terdapat perbedaan bermakna ELB 25%, 50% dan 75% dengan kontrol (+) dan kontrol (-), tidak terdapat perbedaan bermakna antara ELB 25% dengan 50% dan 50% dengan 75%. Sedangkan antara 25% - 75% berbeda bermakna. Sedangkan Uji Post Hoc terhadap P.acne menunjukan bahwa tidak terdapat perbedaan bermakna antara ELB 25%, 50% dan 75% dengan kontrol positif. Kesimpulan : Ekstrak Lidah Buaya (Aloe vera) mempunyai efek antimikroba terhadap isolat bakteri penyebab Acne vulgaris yaitu Propionibacterium acne dan Staphylococcus aureus pada konsentrasi 25%, 50% dan 75% secara invitro.
Occurs E. coli resistance to class 3 cephalosporin class antibiotics and fluoroquinolone groups. The antibiotic resistance that occurs has narrowed the choice of therapy. This study aims to determine ...the effect of Actinomycetes isolates on proteolytic and amylolytic enzymes E. coli ATCC 25922. This research is experimental research, qualitative tests of protease and amylase enzymes from E. coli ATCC 25922 shown by clear zones around the growing colonies. The result of ANOVA One-Way test showed a significant difference in the width of clear zone, colony zone and PER and AER score with p-value < 0,05. This indicates that Actinomycetes isolates contain compounds that can act as inhibitors of protease and amylase enzymes from E.coli ATCC 25922. It is hoped that there will be research about the identification of Actinomycetes species isolates in Bogor Botanical Garden so that later can be cultivated and produced as an antibiotics alternative.
Tobacco leaf contains antibacterial secondary metabolite compounds, such as phenol, alkaloids, and essential oils. This study compares the potential antibacterial effects of Indonesian tobacco leaf ...extracted using the heat reflux method (producing an extract) and pyrolysis method (providing a bio-oil). The tobacco leaf extract was challenged against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853. The bio-oil from the pyrolysis method showed inhibitory Kirby Bauer zones higher than those of the extract from heat reflux method, with the maximum results in the pyrolysis method indicating zones of 6.35 mm (S. aureus), 5.90 mm (E. faecalis), 3.97 mm (E. coli), and 5.025 mm (P. aeruginosa). Further study analyzed the effectiveness of the disc and well diffusion antibacterial test methods for measuring the antibacterial effect of bio-oils against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. The bio-oil used in the well diffusion test method showed the most significant antibacterial effectiveness. It showed the biggest inhibition zone, with a maximum of 11.65 mm and 8.90 mm for E. coli and P. aeruginosa. Our results showed Nicotiana tabacum var. Virginia Bio Oil from Ponorogo (Indonesia) is a strong potential antimicrobial, especially using well diffusion test.
Latar belakang: Prevalensi dismenore diperkirakan berkisar antara 45–95 persen dan 10–25 persen diantaranya merupakan dismenore primer berat. Dismenore dapat menimbulkan sejumlah masalah, antara lain ...limitasi aktivitas, penurunan prestasi akademik, dan kesulitan tidur. Saat ini, terdapat kecenderungan penggunaan herbal dan pengobatan alternatif untuk mengatasi dismenore primer.
Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh madu terhadap tingkat nyeri dismenore dan kualitas hidup mahasiswi Fakultas Kedokteran Universitas Pembangunan Nasional Veteran Jakarta (FKUPNVJ).
Metode: Penelitian menggunakan one group pretest-posttest design. Sebanyak 30 subjek yang ditentukan dengan consecutive sampling diminta minum madu sebanyak dua sendok makan yang dimulai dari dua hari sebelum menstruasi hingga hari ketiga menstruasi. Data diambil menggunakan kuesioner, Numeric Rating Scale, Verbal Multidimensional Scoring System, dan Brief Pain Inventory.
Hasil: Hasil uji Wilcoxon menunjukkan madu dapat menurunkan intensitas nyeri (p = 0,000) dan grade dismenore (p = 0,001). Selain mengurangi derajat nyeri, madu dapat mengurangi lama waktu nyeri menstruasi dari 2 hari menjadi 1 hari (p = 0,001). Hasil uji Wilcoxon juga menunjukkan madu dapat mengurangi gangguan dismenore terhadap aktivitas secara umum, suasana hati, kemampuan berjalan, pekerjaan, hubungan dengan orang lain, tidur, dan menikmati hidup (p ≤ 0,001).
Kesimpulan: madu dapat menurunkan tingkat nyeri dan meningkatkan kualitas hidup mahasiswi FKUPNVJ dengan dismenore primer.
Kata kunci: dismenore primer, kualitas hidup, madu, mahasiswi
Latar belakang: Prevalensi dismenore diperkirakan berkisar antara 45–95 persen dan 10–25 persen diantaranya merupakan dismenore primer berat. Dismenore dapat menimbulkan sejumlah masalah, antara lain ...limitasi aktivitas, penurunan prestasi akademik, dan kesulitan tidur. Saat ini, terdapat kecenderungan penggunaan herbal dan pengobatan alternatif untuk mengatasi dismenore primer.
Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh madu terhadap tingkat nyeri dismenore dan kualitas hidup mahasiswi Fakultas Kedokteran Universitas Pembangunan Nasional Veteran Jakarta (FKUPNVJ).
Metode: Penelitian menggunakan one group pretest-posttest design. Sebanyak 30 subjek yang ditentukan dengan consecutive sampling diminta minum madu sebanyak dua sendok makan yang dimulai dari dua hari sebelum menstruasi hingga hari ketiga menstruasi. Data diambil menggunakan kuesioner, Numeric Rating Scale, Verbal Multidimensional Scoring System, dan Brief Pain Inventory.
Hasil: Hasil uji Wilcoxon menunjukkan madu dapat menurunkan intensitas nyeri (p = 0,000) dan grade dismenore (p = 0,001). Selain mengurangi derajat nyeri, madu dapat mengurangi lama waktu nyeri menstruasi dari 2 hari menjadi 1 hari (p = 0,001). Hasil uji Wilcoxon juga menunjukkan madu dapat mengurangi gangguan dismenore terhadap aktivitas secara umum, suasana hati, kemampuan berjalan, pekerjaan, hubungan dengan orang lain, tidur, dan menikmati hidup (p ≤ 0,001).
Kesimpulan: madu dapat menurunkan tingkat nyeri dan meningkatkan kualitas hidup mahasiswi FKUPNVJ dengan dismenore primer.
Kata kunci: dismenore primer, kualitas hidup, madu, mahasiswi
Dental plaque is formed by colony of bacteria attached to the tooth’s smooth surface. Plaque on the tooth caused by the type of food consumed and the activity of thousands of microorganisms. The main ...microorganisms that forming dental plaque is Streptococcus mutans. If plaque is not removed daily by brushing it will become a hard tartar. Dental plaque can be removed by brushing or flossing between teeth or use mouthwash solution containing antimicrobial agents. One of the types of plant that has the ability to inhibit and kill the microorganisms is clover (Syzigium aromaticum). The aim of this study is to determine the effectiveness of bioactive compounds contained in clove oil isolated microorganisms from dental plaque and the compared with the mouthwash solution containing betel leaf in inhibiting the growth of the bacteria. This study was an experimental research approach post test only control group design with 40 isolates of dental plaque bacteria samples which were selected to find out the type of S.mutans. The result showed that clove oil concentration of 10%; 20%; 30%; dan 40%, have the ability to inhibit S. mutans as well as the other 39 isolates of dental plaque dental bacteria. Based on the analysis of data from the one-way ANOVA test showed significance of 0.05 which means there was no significant difference in the inhibition of clove oil againts dental plaque bacteria isolates of S. mutans and the other 30 isolates of dental plaque in various concentrations.
Background:
Moringa oleifera fruit extract contains beneficial chemical compounds. This study was conducted to observed the power of antioxidant against liver injury by 2-Nitropropane induction in an ...obese male mice model.
Methods: This research was
in vivo laboratory experimental study with a post-test control design group only. The population was obese male mice models, Swiss strain, aged 6-8 weeks, weighing between 60-80 gr. The research sample was determined by Federer's formula for a complete randomized design experimental test, group N (control), O1 (induced by 2-Nitropropane intraperitoneal (i.p) once), O2 (induced by 2-Nitropropane i.p twice), P1 (induced by 2-Nitropropane i.p. once and gavage with
M. oleifera fruit extract 500mg/kg bodyweight (BW) once a day), P2 (induced by 2-Nitropropane i.p. twice and gavage of
M. oleifera fruit extract 500mg/kg BW once a day), and P3 (induced by 2-Nitropropane i.p. twice and gavage of vitamin C 500mg/kg BW once a day). Antioxidant potential parameters were measured by levels of malondialdehide (MDA), glutation (GSH), 8-hydroxy-2'-deoxyguanosine (8-OHdG), catalase activity, manganese superoxide dismutase (MnSOD), serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT). This research was held at the Biochemistry laboratory of Medicine Faculty, UPN Veteran Jakarta in May-September 2020. Analysis was carried out using SPSS version 20.0. The parameters were tested using ANOVA.
Results: MDA levels decreased, GSH increased, 8-OHdG decreased, catalase activity increased, MnSOD activity increased and SGOT, SGPT levels decreased.
M. oleifera fruit extract was statistically proven to be a candidate for potential antioxidant against liver injury of 2-Nitropropane induction in obese male mice model.
Conclusions:
M. oleifera fruit extract was statistically evident as an antioxidant substance that reduces oxidative stress in acute liver injury caused by 2-Nitropropane induction.
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