Abstract Systemic Lupus Erythematosus (SLE) is a heterogeneous autoimmune disease with heightened disease severity in children. The incomplete understanding of the precise cellular and molecular ...events that drive disease activity pose a significant hurdle to the development of targeted therapeutic agents. Here, we performed single-cell phenotypic and functional characterization of pediatric SLE patients and healthy controls blood via mass cytometry. We identified a distinct CD14hi monocyte cytokine signature, with increased levels of monocyte chemoattractant protein-1 (MCP1), macrophage inflammatory protein-1β (Mip1β), and interleukin-1 receptor antagonist (IL-1RA). This signature was shared by every clinically heterogeneous patient, and reproduced in healthy donors' blood upon ex-vivo exposure to plasma from clinically active patients only. This SLE-plasma induced signature was abrogated by JAK1/JAK2 selective inhibition. This study demonstrates the utility of mass cytometry to evaluate immune dysregulation in pediatric autoimmunity, by identification of a multi-parametric immune signature that can be further dissected to delineate the events that drive disease pathogenesis.
A radio bubble shredded by gas sloshing? Botteon, A; Gastaldello, F; ZuHone, J A ...
Monthly notices of the Royal Astronomical Society,
01/2024, Letnik:
527, Številka:
1
Journal Article
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ABSTRACT
We report on the detection of diffuse radio emission with peculiar morphology in the central region of the galaxy cluster Abell 2657. The most striking feature identified in our 144 MHz ...LOFAR image is a bifurcated radio arc that extends for a projected size of 150−200 kpc. From the analysis of XMM-Newton data, we find clear evidence of gas sloshing in the cluster and a possible dip in X-ray surface brightness between the two radio arcs which deserves confirmation. Interestingly, the synchrotron emission of the bifurcated radio arc is stretched along the sloshing spiral. We compare our observational results with numerical simulations of non-thermal components interacting with gas motions. We suggest that the detected emission may trace a radio bubble shredded by gas sloshing, where relativistic electrons and magnetic fields are expected to be stretched and stirred as a consequence of tangential flows induced by the spiralling gas motion. Lastly, we report on the presence of two thin (6−7 kpc in width) and parallel strands of radio emission embedded in the outer arc that are morphologically similar to the emerging population of non-thermal filaments observed in galaxy clusters, radio galaxies, and the Galactic centre. While this work further demonstrates the complex interplay between thermal and non-thermal components in the intracluster medium, follow-up observations in radio and X-rays are required to firmly determine the origin of the features observed in Abell 2657.
Aims. The goal of this work is to devise a description of the enrichment process in large-scale structure that explains the available observations and makes predictions for future measurements. ...Methods. We took a spartan approach to this study, employing observational results and algebra to connect stellar assembly in star-forming halos with metal enrichment of the intra-cluster and group medium. Results. On one hand, our construct is the first to provide an explanation for much of the phenomenology of metal enrichment in clusters and groups. It sheds light on the lack of redshift evolution in metal abundance, as well as the small scatter of metal abundance profiles, the entropy versus abundance anti-correlation found in cool core clusters, and the so-called Fe conundrum, along with several other aspects of cluster enrichment. On the other hand, it also allows us to infer the properties of other constituents of large-scale structure. We find that gas that is not bound to halos must have a metal abundance similar to that of the ICM and only about one-seventh to one-third of the Fe in the Universe is locked in stars. A comparable amount is found in gas in groups and clusters and, lastly and most importantly, about three-fifths of the total Fe is contained in a tenuous warm or hot gaseous medium in or between galaxies. We point out that several of our results follow from two critical but well motivated assumptions: 1) the stellar mass in massive halos is currently underestimated and 2) the adopted Fe yield is only marginally consistent with predictions from synthesis models and SN rates. Conclusions. One of the most appealing features of the work presented here is that it provides an observationally grounded construct where vital questions on chemical enrichment in the large-scale structure can be addressed. We hope that it may serve as a useful baseline for future works.
The intra-cluster medium is prone to turbulent motion that will contribute to the non-thermal heating of the gas, complicating the use of galaxy clusters as cosmological probes. Indirect approaches ...can estimate the intensity and structure of turbulent motions by studying the associated fluctuations in gas density and X-ray surface brightness. In this work, we aim to constrain the gas density fluctuations occurring in the CHEX-MATE sample to obtain a detailed view of their properties in a large population of clusters. To do so, we use a simulation-based approach to constrain the parameters of the power spectrum of density fluctuations, assuming a Kolmogorov-like spectrum and including the stochastic nature of the fluctuation-related observables in the error budget. Using a machine-learning approach, we learn an approximate likelihood for each cluster. This method requires clusters not to be too disturbed, as fluctuations can originate from dynamic processes such as merging. Accordingly, we removed the less relaxed clusters (centroid shift w > 0.02) from our sample, resulting in a sample of 64 clusters. We defined different subsets of CHEX-MATE to determine properties of density fluctuations as a function of dynamical state, mass, and redshift, and we investigated the correlation with the presence or not of a radio halo. We found a positive correlation between the dynamical state and density fluctuation variance, a non-trivial behaviour with mass, and no specific trend with redshift or the presence of a radio halo. The injection scale is mostly constrained by the core region. The slope in the inertial range is consistent with the Kolmogorov theory. When interpreted as originating from turbulent motion, the density fluctuations in R 500 yield an average Mach number of ℳ 3D ≃ 0.4 ± 0.2, an associated non-thermal pressure support of P turb / P tot ≃ (9 ± 6)%, or a hydrostatic mass bias b turb ≃ 0.09 ± 0.06. These findings align with expectations from existing literature.
Galaxy cluster mergers are natural consequences of structure formation in the Universe. Such events involve the dissipation of a large amount of energy (∼10
63
erg) during the process. Part of this ...energy can be channelled in particle acceleration and magnetic field amplification, enhancing non-thermal emission of the intra- and intercluster environment. Recently, low-frequency observations led to the detection of a bridge of diffuse synchrotron emission connecting two merging galaxy clusters, Abell 399 and Abell 401. This result provides clear observational evidence of relativistic particles and magnetic fields in between clusters. In this work, we used LOw Frequency ARray (LOFAR) observations at 144 MHz to study the polarised emission in the A399–A401 bridge region for the first time. No polarised emission was detected from the bridge region. Assuming a model where polarisation is generated by multiple shocks, depolarisation can be due to Faraday dispersion in the foreground medium with respect to the shocks. We constrained its Faraday dispersion to be greater than 0.10 rad m
−2
at 95% confidence level, which corresponds to an average magnetic field in the bridge region of greater than 0.46 nG (or 0.41 nG if we include regions of the Faraday spectrum that are contaminated by Galactic emission). This result is largely consistent with the predictions from numerical simulations for megaparsec regions where the gas density is about 300 times higher than the mean gas density.
Background Activation of Toll-like receptors (TLRs) induces inflammatory responses involved in immunity to pathogens and autoimmune pathogenesis, such as in patients with systemic lupus erythematosus ...(SLE). Although TLRs are differentially expressed across the immune system, a comprehensive analysis of how multiple immune cell subsets respond in a system-wide manner has not been described. Objective We sought to characterize TLR activation across multiple immune cell subsets and subjects, with the goal of establishing a reference framework against which to compare pathologic processes. Methods Peripheral whole-blood samples were stimulated with TLR ligands and analyzed by means of mass cytometry simultaneously for surface marker expression, activation states of intracellular signaling proteins, and cytokine production. We developed a novel data visualization tool to provide an integrated view of TLR signaling networks with single-cell resolution. We studied 17 healthy volunteer donors and 8 patients with newly diagnosed and untreated SLE. Results Our data revealed the diversity of TLR-induced responses within cell types, with TLR ligand specificity. Subsets of natural killer cells and T cells selectively induced nuclear factor κ light chain enhancer of activated B cells in response to TLR2 ligands. CD14hi monocytes exhibited the most polyfunctional cytokine expression patterns, with more than 80 distinct cytokine combinations. Monocytic TLR-induced cytokine patterns were shared among a group of healthy donors, with minimal intraindividual and interindividual variability. Furthermore, autoimmune disease altered baseline cytokine production; newly diagnosed untreated SLE patients shared a distinct monocytic chemokine signature, despite clinical heterogeneity. Conclusion Mass cytometry defined a systems-level reference framework for human TLR activation, which can be applied to study perturbations in patients with inflammatory diseases, such as SLE.
We investigate the statistical properties and the origin of the scatter within the spatially resolved surface brightness profiles of the CHEX–MATE sample, formed by 118 galaxy clusters selected via ...the SZ effect. These objects have been drawn from the
Planck
SZ catalogue and cover a wide range of masses,
M
500
= 2 − 15×10
14
M
⊙
, and redshift,
z
= 0.05, 0.6. We derived the surface brightness and emission measure profiles and determined the statistical properties of the full sample and sub-samples according to their morphology, mass, and redshift. We found that there is a critical scale,
R
∼ 0.4
R
500
, within which morphologically relaxed and disturbed object profiles diverge. The median of each sub-sample differs by a factor of ∼10 at 0.05
R
500
. There are no significant differences between mass- and redshift-selected sub-samples once proper scaling is applied. We compare CHEX–MATE with a sample of 115 clusters drawn from the T
HE
T
HREE
H
UNDRED
suite of cosmological simulations. We found that simulated emission measure profiles are systematically steeper than those of observations. For the first time, the simulations were used to break down the components causing the scatter between the profiles. We investigated the behaviour of the scatter due to object-by-object variation. We found that the high scatter, approximately 110%, at
R
< 0.4
R
500
Y
SZ
is due to a genuine difference between the distribution of the gas in the core of the clusters. The intermediate scale,
R
500
Y
SZ
= 0.4−0.8, is characterised by the minimum value of the scatter on the order of 0.56, indicating a region where cluster profiles are the closest to the self-similar regime. Larger scales are characterised by increasing scatter due to the complex spatial distribution of the gas. Also for the first time, we verify that the scatter due to projection effects is smaller than the scatter due to genuine object-by-object variation in all the considered scales.
The “Cluster HEritage project with XMM-Newton : Mass Assembly and Thermodynamics at the End point of structure formation” (CHEX-MATE) is a multi-year heritage program to obtain homogeneous XMM-Newton ...observations of a representative sample of 118 galaxy clusters. The observations are tuned to reconstruct the distribution of the main thermodynamic quantities of the intra-cluster medium up to R 500 and to obtain individual mass measurements, via the hydrostatic-equilibrium equation, with a precision of 15−20%. Temperature profiles are a necessary ingredient for the scientific goals of the project and it is thus crucial to derive the best possible temperature measurements from our data. This is why we have built a new pipeline for spectral extraction and analysis of XMM-Newton data, based on a new physically motivated background model and on a Bayesian approach with Markov chain Monte Carlo methods, which we present in this paper for the first time. We applied this new method to a subset of 30 galaxy clusters representative of the CHEX-MATE sample and show that we can obtain reliable temperature measurements up to regions where the source intensity is as low as 20% of the background, keeping systematic errors below 10%. We compare the median profile of our sample and the best-fit slope at large radii with literature results and we find a good agreement with other measurements based on XMM-Newton data. Conversely, when we exclude the most contaminated regions, where the source intensity is below 20% of the background, we find significantly flatter profiles, in agreement with predictions from numerical simulations and independent measurements with a combination of Sunyaev–Zeldovich and X-ray imaging data.
The connection between the thermal and non-thermal properties in galaxy clusters hosting radio halos seems fairly well established. However, a comprehensive analysis of such a connection has only ...been done for integrated quantities (e.g. L X − P radio relation). In recent years, new-generation radio telescopes have enabled the unprecedented possibility to study the non-thermal properties of galaxy clusters on a spatially resolved basis. In this work, we performed a pilot study to investigate the mentioned properties on five targets by combining X-ray data from the CHEX-MATE project with the second data release from the LOFAR Two meter Sky survey. We find a strong correlation ( r s ∼ 0.7) with a slope less than unity between the radio and X-ray surface brightness. We also report differences in the spatially resolved properties of the radio emission in clusters that show different levels of dynamical disturbance. In particular, less perturbed clusters (according to X-ray parameters) show peaked radio profiles in the centre, with a flattening in the outer regions, while the three dynamically disturbed clusters have steeper profiles in the outer regions. We fitted a model to the radio emission in the context of turbulent re-acceleration with a constant ratio between thermal and non-thermal particles’ energy densities and a magnetic field profile linked to the thermal gas density as B ( r ) ∝ n th 0.5 . We found that this simple model cannot reproduce the behaviour of the observed radio emission.
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