This study was designed to evaluate a spray-dried multivalent polyclonal antibody preparation (PAP) against lactate-producing bacteria as an alternative to monensin (MON) to control ruminal ...acidification. Holstein cows (677 ± 98 kg) fitted with ruminal cannulas were allocated in an incomplete Latin square design with two 20 days period. Cows were randomly assigned to control (CTL), PAP, or MON treatments. For each period, cows were fed a forage diet in the first 5 days (d-5 to d-1), composed of sugarcane, urea and a mineral supplement, followed by a 74% concentrate diet for 15 days (d 0 to d 14). There were no treatment main effects (
> 0.05) on dry matter intake (DMI) and microbial protein synthesis. However, there was a large peak (
< 0.01) of intake on d 0 (18.29 kg), followed by a large decline on d 1 (3.67 kg). From d2, DMI showed an increasing pattern (8.34 kg) and stabilized around d 8 (12.96 kg). Higher mean pH was measured (
< 0.01) in cattle-fed MON (6.06 vs. PA
= 5.89 and CTL = 5.91). The ruminal NH
-N concentration of CTL-fed cows was lower (
< 0.01) compared to those fed MON or PAP. The molar concentration of acetate and lactate was not affected (
> 0.23) by treatments, but feeding MON increased (
= 0.01) propionate during the first 4 days after the challenge. Feeding MON and PAP reduced (
= 0.01) the molar proportion of butyrate. MON was effective in controlling pH and improved ruminal fermentation of acidosis-induced cows. However, PAP was not effective in controlling acidosis. The acidosis induced by the challenge was caused by the accumulation of SCFAs. Therefore, the real conditions for evaluation of this feed additive were not reached in this experiment, since this PAP was proposed to work against lactate-producing bacteria.
Global marine conservation remains fractured by an imbalance in research efforts and policy actions, limiting progression towards sustainability. Rhodolith beds represent a prime example, as they ...have ecological importance on a global scale, provide a wealth of ecosystem functions and services, including biodiversity provision and potential climate change mitigation, but remain disproportionately understudied, compared to other coastal ecosystems (tropical coral reefs, kelp forests, mangroves, seagrasses). Although rhodolith beds have gained some recognition, as important and sensitive habitats at national/regional levels during the last decade, there is still a notable lack of information and, consequently, specific conservation efforts. We argue that the lack of information about these habitats, and the significant ecosystem services they provide, is hindering the development of effective conservation measures and limiting wider marine conservation success. This is becoming a pressing issue, considering the multiple severe pressures and threats these habitats are exposed to (e.g., pollution, fishing activities, climate change), which may lead to an erosion of their ecological function and ecosystem services. By synthesizing the current knowledge, we provide arguments to highlight the importance and urgency of levelling-up research efforts focused on rhodolith beds, combating rhodolith bed degradation and avoiding the loss of associated biodiversity, thus ensuring the sustainability of future conservation programs.
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•Rhodolith beds (RBs) are globally extensive coastal habitats.•We identify key features of RBs to qualify them as a global conservation priority.•Research efforts on RBs lag far behind other coastal habitats.•The lack of information hampers conservation of RBs, which is non-existent in most regions.•This study calls for levelling-up research efforts to reach RB conservation needs.
The present study aimed to evaluate the effects of different doses of a spray-dried polyclonal antibody preparation against specific ruminal bacteria on rumen fermentation parameters as well as ...protozoa counts, in situ degradability of sugarcane neutral detergent fibre (NDF) and total tract apparent digestibility of total diet in cows fed high concentrate diets. Eight ruminally-cannulated cows were used in a replicated 4×4 Latin square design with four experimental periods of 21 days. The treatments were: T1 (control), 0.0 g/d of multivalent polyclonal antibody preparation (PAP-MV); T2, 1.5 g/d of PAP-MV; T3, 3.0 g/d of PAP-MV; T4, 4.5 g/d of PAP-MV. Sample collection for rumen fermentation parameters was carried out the last day of each period at 0, 2, 4, 6, 8, 10 and 12 h after morning meal. For protozoa counts, samples were collected the last day of each period at 0 and 4 h after feeding. In situ degradability of sugarcane NDF was performed the last 5 days of each period, while total tract apparent digestibility of total diet was assessed the last 10 days of each period. Regardless of sampling time, there was no linear or quadratic effect on rumen pH, total concentration of short chain fatty acids, molar proportion of acetate, propionate and butyrate, ammonia nitrogen (NH
3
-N) or lactate. No treatment effects were detected for protozoa counts, in situ degradability of sugarcane NDF and total tract apparent digestibility of total diet. In conclusion, different levels of spray-dried PAP-MV were not sufficient to alter rumen environment.
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e16056
Background: Partial nephrectomy (PN) is the standard of care in the management of cT1a tumors, while radical nephrectomy (RN) is indicated in more advanced tumors. Recent studies ...provided evidence that PN could be performed in patients with tumors greater that 7 cm with complication rates and oncological outcomes comparable with those undergoing RN. This study compares the recurrence-free survival (RFS), overall (OS) and cancer-specific survival (CSS) of PN and RN in patients with non-metastatic pathological T3a renal cell carcinoma (RCC) with perirenal fat invasion only. Methods: We reviewed 1202 patients undergoing RN (n = 653) and PN (n = 549), at a oncological referral center, from January 2003 to June 2016. Of all patients, we identified 25 RN and 41 PN pT3a tumors with exclusively perirenal fat invasion. None had nodal or distant metastasis at pretreatment clinical staging. Patients characteristics were compared with Mann-Whitney U test and Student t-test for categorical and numeric variables with normal distribution, respectively. Both groups were compared for RFS, OS and CSS with a Kaplan-Meier survival analysis. Results: All patients included had pT3a stage with isolated perirenal fat invasion. Groups undergoing RN and PN were not significantly different regarding Charlson Comorbidity Index (Median 3 for RN vs 4 for PN, p = 0.24) or Age (Mean 65.3 for RN vs 62.0 for PN, p = 0.99). Patients undergoing RN had bigger tumors (7.9 cm vs 4.6, p < 0.001) and higher Fuhrman grade (p = 0.01). Median follow-up was 36 months for RN and 34 months for PN. At the end of follow-up, recurrence was seen in 3 patients undergoing RN (12%) and 2 undergoing PN (5%), p = 0.36. Mortality was similar across groups (16% for RN vs 15% for PN, p = 0.99) as well as Cancer-specific mortality (4% for RN vs. 5% for PN, p = 0.99). At the end of follow-up, RFS was 80% (20/25) for RN and 82% (34/41) for PN. Conclusions: In our data, renal cell carcinoma with T3 stage due to perirenal fat invasion exclusively had similar outcomes when treated with Radical or Partial Nephrectomy. OS as well as RFS were comparable for both surgical modalities, suggesting that, although RN is currently the gold standard for this staging, PN may provide similar oncologic results.
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