The characteristics of acoustic-gravity waves (waveforms, time durations, amplitudes, azimuths and horizontal phase speeds) from the eruption of the Hunga-Tonga-Hunga-Hapai volcano detected at ...different infrasound stations of the Infrasound Monitoring System and at a network of low-frequency microbarographs in the Moscow region are studied. Using the correlation analysis of the signals at different locations, six arrivals of signals from the volcano, which made up to two revolutions around the Earth, were detected. The Lamb mode of acoustic gravity waves from the volcano eruption is identified and the effect of this mode on generation of tsunami waves and variation of aerosol concentration is studied. The energy released from an underwater volcano into the atmosphere is estimated from the parameters of the Lamb wave and compared with the energy released from the most powerful nuclear bomb of 58 Mt TNT.
Tick-borne encephalitis virus (TBEV) is transmitted to vertebrates by taiga or forest ticks through bites, inducing disease of variable severity. The reasons underlying these differences in the ...severity of the disease are unknown. In order to identify genetic factors affecting the pathogenicity of virus strains, we have sequenced and compared the complete genomes of 34 Far-Eastern subtype (FE) TBEV strains isolated from patients with different disease severity (Primorye, the Russian Far East). We analyzed the complete genomes of 11 human pathogenic strains isolated from the brains of dead patients with the encephalitic form of the disease (Efd), 4 strains from the blood of patients with the febrile form of TBE (Ffd), and 19 strains from patients with the subclinical form of TBE (Sfd). On the phylogenetic tree, pathogenic Efd strains formed two clusters containing the prototype strains, Senzhang and Sofjin, respectively. Sfd strains formed a third separate cluster, including the Oshima strain. The strains that caused the febrile form of the disease did not form a separate cluster. In the viral proteins, we found 198 positions with at least one amino acid residue substitution, of which only 17 amino acid residue substitutions were correlated with the variable pathogenicity of these strains in humans and they authentically differed between the groups. We considered the role of each amino acid substitution and assumed that the deletion of 111 amino acids in the capsid protein in combination with the amino acid substitutions R16K and S45F in the NS3 protease may affect the budding process of viral particles. These changes may be the major reason for the diminished pathogenicity of TBEV strains. We recommend Sfd strains for testing as attenuation vaccine candidates.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The strain
sp. SLB01 was isolated from the diseased freshwater sponge
(Pallas, 1776) and the draft genome was published previously. The aim of this work is to analyze the genome of the
sp. SLB01 to ...search for pathogenicity factors for Baikal sponges. We performed genomic analysis to determine virulence factors, comparing the genome of the strain SLB01 with genomes of other related
strains from the environment. The strain
sp. SLB01 contained genes encoding violacein, alpha-amylases, phospholipases, chitinases, collagenases, hemolysin, and a type VI secretion system. In addition, the presence of conservative clusters of genes for the biosynthesis of secondary metabolites of tropodithietic acid and marinocine was found. We present genes for antibiotic resistance, including five genes encoding various lactamases and eight genes for penicillin-binding proteins, which are conserved in all analyzed strains. Major differences were found between the
sp. SLB01 and
strains in the spectra of genes for glycosyltransferases and glycoside hydrolases, serine hydrolases, and trypsin-like peptidase, as well as some TonB-dependent siderophore receptors. Thus, the study of the analysis of the genome of the strain SLB01 allows us to conclude that the strain may be one of the pathogens of freshwater sponges.
Abstract
We propose the design of a dual-pixel array of Schottky diodes. Each diode is fixed between the bow-tie antenna arms on top of a SI-GaAs membrane acting as a waveguide backshort for ...efficient coupling of the antenna to the feedline of high-directivity horn. The detector utilizes a single RF output line: microwave reflectometer is used for the readout. The pixels are equipped with dual-mode resonator filters to eliminate the cross-talk. We evaluate the design proposed via numerical simulation and performance tests of the array subunits:
NEP
of 300 pW/Hz
0.5
and dynamic range of 24 dB are demonstrated at 137.5 GHz.
Anthropogenic pollution of the atmosphere with organic and inorganic gaseous species has been studied using constant high-quality monitoring of the atmosphere composition both in megacity of Moscow ...and in its countryside. The article considers continuous measurements of the main climatically and chemically active atmospheric gaseous species concentrations, including volatile organic compounds. The main attention is paid to the comparative analysis, mainly between the megacity and its suburban area, by average species concentrations and some quality features of their seasonal and diurnal variations. The obtained results confirmed the previously studied features of the daily variations of inorganic gaseous species in Moscow and showed such features for organic compounds in the countryside.
To date, a lot of data on molecular genetic characteristics of different tick-borne encephalitis virus strains has appeared. Only on the basis of the E protein genome, sequences of about 1,500 TBEV ...strains were registered in GenBank.
The purpose of the work - revision and comparative analysis of data on complete genomes sequences of the Far Eastern subtype of TBE virus strains distributed in the Asian part of Eurasian continent.
The data on the complete genomes of 84 strains of TBEV isolated in Asia were used; phylogenetic analysis was performed.
it was shown that variants of the TBEV of the Far Eastern subtype are circulating here and form three separate clusters (Sofjin, Senzhang- и Shkotovo-like strains). Sofjin strain (Sofjin-1953, Sofjin-Chumakov, Sofjin-KSY) was considered to be the reference for Far Eastern TBE virus subtype strains and a cluster of Sofjin-like strains. Sofjin-like strains were not found in China and Japan, but widely distributed throughout the area of Primorsky and Khabarovsk krai. The group of Senzhang-like strains was distributed in China, Eastern Siberia, Khabarovsk krai and northern Primorsky krai, but was not found in Japan (Hokkaido). According to molecular genetic characteristics the youngest and more genetically homogeneous group was the Shkotovo-like strains, isolated in the southern part of Primorsky krai, however not found on Hokkaido Island (Japan).
revision of the complete genome characteristics of TBEV strains revealed the features of micro-evolutionary process of viral populations in the Asian part of Eurasia, show the individual affection of strains to certain territories, as well as detect random finds of such strains in the territories of other natural foci.
In this paper we report on the results of investigation of efficiency of a microwave reflectometry for readout of a terahertz multipixel Schottky diode direct detector. Decent capabilities of the ...microwave reflectometry readout were earlier justified by us for a hot electron bolometric direct detector. In case of a planar Schottky diode, we observed increase of an optical noise equivalent power by a factor of 2 compared to that measured within a conventional readout scheme. For implementation of a multipixel camera, a microwave reflectometer is to be used to readout each row of the camera, and the row switching is to be maintained by a CMOS analog multiplexer. The diodes within a row have to be equipped with filters to distribute the probing microwave signal properly. The simultaneous use of analog multiplexing and microwave reflectometry enables to reduce the camera response time by a factor of its number of columns.
Lake Baikal is a unique oligotrophic freshwater lake with unusually cold conditions and amazing biological diversity. Studies of the lake's viral communities have begun recently, and their full ...diversity is not elucidated yet. Here, we performed DNA viral metagenomic analysis on integral samples from four different deep-water and shallow stations of the southern and central basins of the lake. There was a strict distinction of viral communities in areas with different environmental conditions. Comparative analysis with other freshwater lakes revealed the highest similarity of Baikal viromes with those of the Asian lakes Soyang and Biwa. Analysis of new data, together with previously published data allowed us to get a deeper insight into the diversity and functional potential of Baikal viruses; however, the true diversity of Baikal viruses in the lake ecosystem remains still unknown. The new metaviromic data will be useful for future studies of viral composition, distribution, and the dynamics associated with global climatic and anthropogenic impacts on this ecosystem.
The results from first series of measurements performed by the ALICE experiment at the LHC are presented. These measurements include the charged-particle pseudorapidity densities, multiplicity ...distributions and transverse momentum spectra obtained by analyzing the data collected in 2009 and 2010 in proton-proton collisions at three different center-of-mass energies of 0.9, 2.36, and 7 TeV. The results are compared to previous proton-antiproton data and to model predictions.
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