Abstract The aim of the present work was to evaluate and analyze the growth and mineral nutrition response of stone pine (Pinus pinea L.) seedlings, an economically important forest species. We ...analyzed the salinity effects on the kinetics of growth, development, and absorption of nutrients of plants cultivated under controlled conditions on a solid organic substrate. Pinus pinea plants were able to tolerate 25 mM NaCl concentration without reduced growth compared to the non-saline control. However, the salt concentration of 50 mM significantly affected the seedling growth after two weeks of treatment. Root growth activity was decreased more than the aerial parts at applied NaCl concentrations. On the other hand, seedlings restricted the transport of Na+ ions to the aerial parts and were strongly selective in favour of K+ ions. The presence of NaCl in the culture medium decreased the absorption rate and the export of K+ and Na+ ions to the aerial parts. This was reflected in the accumulation way of these two ions in the whole plant.
Resumo O objetivo do presente trabalho foi avaliar e analisar a resposta de crescimento e nutrição mineral de mudas de pinheiro-manso (Pinus pinea L.), uma espécie florestal economicamente importante. Analisamos os efeitos da salinidade na cinética de crescimento, desenvolvimento e absorção de nutrientes de plantas cultivadas sob condições controladas em substrato orgânico sólido. As plantas de P. pinea foram capazes de tolerar a concentração de 25 mM de NaCl sem redução do crescimento em comparação com o controle não salino. No entanto, a concentração de sal de 50 mM afetou significativamente o crescimento das plântulas após duas semanas de tratamento. A atividade de crescimento das raízes foi mais reduzida do que as partes aéreas nas concentrações de NaCl aplicadas. Por outro lado, as plântulas restringiram o transporte de íons Na+ para a parte aérea e foram fortemente seletivas em favor dos íons K+. A presença de NaCl no meio de cultura diminuiu a taxa de absorção e a exportação de íons K+ e Na+ para as partes aéreas. Isso se refletiu na forma de acúmulo desses dois íons em toda a planta.
The effect of extracts from leaves of
Myrtus communis on the SOS reponse induced by Aflatoxin B1 (AFB1) and Nifuroxazide was investigated in a bacterial assay system, i.e. the SOS chromotest with
...Escherichia coli PQ37. Aqueous extract, the total flavonoids oligomer fraction (TOF), hexane, chloroform, ethyl acetate and methanol extracts and essential oil obtained from
M. communis significantly decreased the SOS response induced by AFB1 (10
μg/assay) and Nifuroxazide (20
μg/assay). Ethyl acetate and methanol extracts showed the strongest inhibition of the induction of the SOS response by the indirectly genotoxic AFB1. The methanol and aqueous extracts exhibited the highest level of protection towards the SOS-induced response by the directly genotoxic Nifuroxazide. In addition to anti-genotoxic activity, the aqueous extract, the TOF, and the ethyl acetate and methanol extracts showed an important free-radical scavenging activity towards the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. These results suggest the future utilization of these extracts as additives in chemoprevention studies.
Context:
Cardiac dysfunction is one of the most serious consequences of scorpion envenomation. The best tool to evaluate cardiac function is echocardiography, but it is not available at all emergency ...departments. Many studies aimed to describe biological predictive factors of cardiac dysfunction in scorpion envenomation. Troponin is one of these biomarkers but its correlation with myocarditis is not well established. The aim of this study was to evaluate correlation between troponin levels and cardiac dysfunction in moderate scorpion envenomation.
Methods:
A retrospective monocentric study including patients admitted in the emergency department for moderate scorpion envenomation with troponin measurement during their early management. On arrival, an electrocardiogram and a chest X-ray were realized for all patients.
Results:
We enrolled 132 patients with a mean age at 31.3 ± 24.4 years and a 1.35 sex-ratio. All patients had moderate systemic manifestations. There were 28 patients with clinical manifestations of cardiac dysfunction without life-threatening troubles (21.2%). Troponin was undetectable in 69 patients (56%). The mean value of troponin level (pg/ml) was higher in patients with clinical manifestations of left ventricular dysfunction (1.80 ± 3.8 vs. 0.11 ± 0.5; p = 0.02). Troponin levels were significantly higher in patients with positive T wave on electrocardiogram.
Conclusion:
In patients with moderate scorpion envenomation with positive T wave, high values of troponin suggest the presence of cardiac dysfunction.
To improve time of surfactant administration with a surfactant replacement protocol based on semiquantitative lung ultrasound score (LUS) thresholds.
Quality improvement (QI), prospective, ...before-after, pilot study. In a 6-month period surfactant replacement was based only on inspired oxygen fraction (FiO2) thresholds. In the second 6-month period, surfactant was given when either the FiO2 or LUS exceeded the limits. The main QI measures were the proportion of neonates receiving surfactant within the first 3 hours of life and maximal FiO2 reached before surfactant replacement. Secondary QI measures were the duration of respiratory support and ventilator-free days. Data were also collected for 1 year after the study to verify sustainability.
Echography-guided Surfactant THERapy (ESTHER) increased the proportion of neonates receiving surfactant within the first 3 hours of life (71.4%-90%; P < .0001) and reduced the maximal FiO2 reached before surfactant replacement (0.33 0.26-0.5) vs 0.4 0.4-0.55; P = .005). The global need for surfactant did not significantly change. ESTHER also resulted in a significant decrease in duration of invasive ventilation and ventilator-free days.
ESTHER improved the timeliness of surfactant administration and secondary QI indicators related to surfactant replacement.
The
in vitro antibacterial, antioxidant, cytotoxic and apoptotic activities from tubers extracts of
Cyperus rotundus (Cyperaceae) were investigated. Antibacterial activity of different extracts was ...evaluated against five bacterial reference strains. A marked inhibitory effect was observed against
Salmonella enteritidis,
Staphylococcus aureus and
Enterococcus faecalis with total oligomers flavonoids (TOFs) and ethyl acetate extracts. In addition to their antibacterial activity, the same extracts showed a significant ability to inhibit nitroblue tetrazolium reduction by the superoxide radical in a non-enzymatic superoxide generating system. Apoptosis, a highly organized physiological mechanism to eliminate injured or abnormal cells, is also implicated in multistage carcinogenesis. It was observed that TOF and ethyl acetate extracts suppressed growth and proliferation of L1210 cells derived from murine lymphoblastic leukaemia. Morphological features of treated cells and characteristic DNA fragmentation revealed that the cytotoxicity was due to induction of apoptosis. This study confirms that TOF and ethyl acetate extracts of
C.
rotundus possess antibacterial and antioxidant properties and provoke DNA fragmentation, a sign of induction of apoptosis. These results were correlated with chemical composition of the tested extracts.
Fractionation of the methanolic and total oligomer flavonoid enriched extracts from
Rhamnus alaternus leaves resulted in the isolation of three flavonoids: kaempferol 3-
O-isorhamninoside (
1), ...rhamnocitrin-3-
O-isorhamninoside (
2) and rhamnetin-3-
O-isorhamninoside (
3), along with apigenin, kaempferol and quercetin. The structures were determined using data obtained from FAB–MS,
1H and
13C NMR spectra, as well as by various correlation experiments (COSY, HMQC and HMBC). The antioxidant activities of the isolated compounds were evaluated by measuring their ability to scavenge the DPPH radical and superoxide anions, to inhibit H
2O
2-induced lipid peroxidation in human K562 cells, and to inhibit xanthine oxidase activity. Compound
3 was a strong scavenger of DPPH
and superoxide anion radicals, and a potent inhibitor of H
2O
2-induced lipid peroxidation, with respective
IC
50 values of 1.5, 35 and 106
μg/ml, whereas compound
1 showed the better activity in the inhibition of xanthine oxidase activity with an
IC
50 value of 18
μg/ml, showing some structure–activity relationships.
Genotoxic and antigenotoxic activities against direct acting mutagen nifuroxazide, and indirect acting genotoxin aflatoxine B1 (AFB1) of extracts prepared from fruits of Pistacia lentiscus, were ...studied using the SOS chromotest with Escherichia coli PQ 37 as a tester organism. It was found that different extracts showed no genotoxicity when tested with E. coli PQ 37 strain either with or without microsomal extract. On the other hand, our results showed that ethyl acetate fraction and total oligomers flavonoids (TOF) extract obtained from P. lentiscus significantly decreased the genotoxicicity induced by AFB1 (10 μg/assay) and nifuroxazide (20 μg/assay). In addition to antigenotoxic activity, these extracts showed an important free radical scavenging activity towards the 2,2-diphenyl-2-picrylhydrazyl (DPPH) free radical and an important antioxidant activity using xanthine/xanthine oxidase system.
Mucormycosis is a rare and often fatal fungal infection caused by fungi of the Mucorales order. It is mostly seen in people with weakened immune systems or with uncontrolled diabetes, and can cause ...extensive soft tissue damage and decay. Hemophagocytic lymphohistiocytosis has been reported only rarely and never described in an intensive care or emergency department setting. The purpose of this report is to describe a fatal case of sinonasal mucormycosis complicated by hemophagocytic lymphohistiocytosis in a diabetic child.
A mail questionnaire was completed by 171 patients two to 20 months after undergoing coronary artery bypass graft surgery (CABG). The post-CABG period was characterized by fewer working hours, a ...higher level of physical exercise, a reduction in smoking, and more appropriate nutritional habits, compared with the preoperation period. At the same time, the anxiety level of post-CABG patients was higher than that measured in a community sample. Post-CABG high psychological distress (anxiety and mood states) and low functional capacity were associated with high levels of pessimism and ineffective emotion-focused coping strategies. These results may be used by social workers in devising psychological interventions aimed at improving post-CABG patients' quality of life and bolstering their coping strategies.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, ODKLJ, OILJ, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK, VSZLJ
This study evaluates mutagenic and antimutagenic effects of aqueous, total oligomers flavonoïds (TOF), ethyl acetate and methanol extracts from aerial parts of
Cyperus rotundus with the
Salmonella ...typhimurium assay system.
The different extracts showed no mutagenicity when tested with
Salmonella typhimurium strains TA98, TA100, TA1535 and TA1538 either with or without the S9 mix. On the other hand, our results showed that all extracts have antimutagenic activity against Aflatoxin B1 (AFB1) in TA100 and TA98 assay system, and against sodium azide in TA100 and TA1535 assay system. TOF, ethyl acetate and methanol extracts exhibited the highest inhibition level of the Ames response induced by the indirect mutagen AFB1. Whereas, ethyl acetate and methanol extracts exhibited the highest level of protection towards the direct mutagen, sodium azide, induced response. In addition to antimutagenic activity, these extracts showed an important free radical scavenging activity towards the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. TOF, ethyl acetate and methanol extracts showed IC
50 value of 15, 14 and 20
μg/ml, respectively.
Taken together, our finding showed that
C. rotundus exhibits significant antioxidant and antimutagenic activities.
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