Introduction
Recent automated hematology analyzers (HAs) can identify and report nucleated red blood cells (NRBC) count as a separate population out of white blood cells (WBC). The aim of this study ...was to investigate the analytical performances of NRBC enumeration on five top of the range HAs.
Methods
We evaluated the within‐run and between‐day precision, limit of blank (LoB), limit of detection (LoD), and limit of quantitation (LoQ) of XE‐2100 and XN‐module (Sysmex), ADVIA 2120i (Siemens), BC‐6800 (Mindray), and UniCel DxH 800 (Beckman Coulter). Automated NRBC counts were also compared with optical microscopy (OM).
Results
The limits of detection for NRBC of the BC‐6800, XN‐module, XE‐2100, UniCel DxH 800, and ADVIA 2120i are 0.035×109/L, 0.019×109/L, 0.067×109/L, 0.038×109/L, and 0.167×109/L, respectively. Our data indicated excellent performance in terms of precision. The agreement with OM was excellent for BC‐6800, XN‐module, and XE‐2100 (Bias 0.023, 0.019, and 0.033×109/L, respectively). ADVIA 2120i displayed a significant constant error and UniCel DxH 800 both proportional and small constant error.
Conclusion
Regards to NRBC counting, the performances shown by BC‐6800, XN‐module, and XE‐2100 are excellent also a low count, ADVIA 2120i and UniCel DxH 800 need to be improved.
Background
The aims of this study were to compare the diagnostic accuracy of blood smear review criteria, by means of three different panel rules, those proposed by: the International Consensus Group ...for Hematology 41‐ICGH rules, the Italian Survey IS rules and the Working Group on Hematology‐SIBioC (WGH) consensus rules (WGH rules).
Methods
This study is based on 2707 peripheral blood (PB) samples referred for routine hematological testing to the WGH‐associated laboratories displaced all over the Italian territory. The PB samples were processed on seven different hematology analyzers (HAs): Advia 2120i, XE‐2100, BC‐6800, ABX Pentra, XN‐1000, Cell‐DYN Sapphire, and DxH800, respectively. All the results provided by the HAs were analyzed through the application of three different blood smear review criteria: that is, the 41‐ICGH, IS, and WGH rules. Finally, data were compared with those obtained by optical microscopy (OM), as the current gold standard.
Results
The overall the agreement OM classification with ICGH, IS, and WGH panel rules is 0.83, 0.83, and 0.85, respectively. The false negatives are 2.1%, 3.0%, and 2.9%, while false positives are 15.1%, 13.7%, and 11.7%, respectively. All the seven HAs showed variable interinstrument performance, as three different criteria for OM review were adopted on each of them from time to time.
Conclusion
These results presented show that the customization of validation rules is necessary for enhancing the quality of hematological testing and optimizing workflow.
Diurnal gene expression patterns underlie time-of-the-day-specific functional specialization of tissues. However, available circadian gene expression atlases of a few organs are largely from ...nocturnal vertebrates. We report the diurnal transcriptome of 64 tissues, including 22 brain regions, sampled every 2 hours over 24 hours, from the primate
(baboon). Genomic transcription was highly rhythmic, with up to 81.7% of protein-coding genes showing daily rhythms in expression. In addition to tissue-specific gene expression, the rhythmic transcriptome imparts another layer of functional specialization. Most ubiquitously expressed genes that participate in essential cellular functions exhibit rhythmic expression in a tissue-specific manner. The peak phases of rhythmic gene expression clustered around dawn and dusk, with a "quiescent period" during early night. Our findings also unveil a different temporal organization of central and peripheral tissues between diurnal and nocturnal animals.
In the last two decades, the promotion of agro-industry has become a dominant developmental imperative on the African continent, leading to efforts to involve private-sector actors. This article ...examines the political economy and ecology of agro-industry in the Senegal River delta, focusing on local-level reactions to Senegalese initiatives aimed at attracting foreign investors in agriculture. The argument is that Senegal is witnessing the emergence of an agro-extractivist pattern that replaces earlier development objectives - such as peasants' integration into the national economy - with the new imperative of the integration of territories into global capitalism. The article presents empirical evidence on three main consequences of the increased presence of agro-industry: a process of change in land property and access; the end of public support to peasant farmers; and an intensified marginalisation of pastoralism. Colonial heritage and the role of local resistances in shaping and mediating this developmental strategy are also discussed.
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