Important evidence is reported on the antimicrobial and antagonistic properties of bacterial endophytes in Echinacea purpurea and their role in the modulation of plant synthesis of bioactive ...compounds. Here, endophytic fungi were isolated from E. purpurea, and the dual culture approach was applied to deepen insights into the complex plant–microbiome interaction network.
In vitro experiments were carried out to evaluate the species specificity of the interaction between host (E. purpurea) and non‐host (E. angustifolia and Nicotiana tabacum) plant tissues and bacterial or fungal endophytes isolated from living E. purpurea plants to test interactions between fungal and bacterial endophytes.
A higher tropism towards plant tissue and growth was observed for both fungal and bacterial isolates compared to controls without plant tissue. The growth of all fungi was significantly inhibited by several bacterial strains that, in turn, were scarcely affected by the presence of fungi. Finally, E. purpurea endophytic bacteria were able to inhibit mycelial growth of the phytopathogen Botrytis cinerea.
Bacteria and fungi living in symbiosis with wild Echinacea plants interact with each other and could represent a potential source of bioactive compounds and a biocontrol tool.
Bacteria and fungi living in symbiosis with wild Echinacea plants interact at cellular level with each other and could represent a new source of bioactive compounds and a biocontrol tool.
Abstract In this work, we demonstrate in a proof of concept experiment the efficient noise absorption of a 3D printed panel designed with appropriately arranged space-coiling labyrinthine acoustic ...elementary cells of various sizes. The labyrinthine unit cells are analytically and numerically analysed to determine their absorption characteristics and then fabricated and experimentally tested in an impedance tube to verify the dependence of absorption characteristics on cell thickness and lateral size. The resonance frequency of the unit cell is seen to scale approximately linearly with respect to both thickness and lateral size in the considered range, enabling easy tunability of the working frequency. Using these data, a flat panel is designed and fabricated by arranging cells of different dimensions in a quasi-periodic lattice, exploiting the acoustic ‘rainbow’ effect, i.e. superimposing the frequency response of the different cells to generate a wider absorption spectrum, covering the target frequency range, chosen between 800 and 1400 Hz. The panel is thinner and more lightweight compared to traditional sound absorbing solutions and designed in modular form, so as to be applicable to different geometries. The performance of the panel is experimentally validated in a small-scale reverberation room, and an absorption close to ideal values is demonstrated at the desired frequencies of operation. Thus, this work suggests a design procedure for noise-mitigation panel solutions and provides experimental proof of the versatility and effectiveness of labyrinthine metamaterials for tunable mid- to low-frequency sound attenuation.
This report documents a case of bisphosphonate-related osteonecrosis of the jaw (BRONJ) after dental implant placement in an osteoporotic patient treated with alendronate for 6 years. A 63-year-old ...patient underwent rehabilitation of the posterior mandible with 2 dental implants in 2006 while taking alendronate to treat osteoporosis. The surgical procedure was uneventful. Both implants integrated well, and in November 2006 the patient wore a fixed partial prosthesis. Alendronate was never discontinued. In June 2008 a painful cheek swelling of the left mandible developed, associated with gingival bleeding. Since then, the patient underwent several courses of antibiotics, without relief of symptoms. In June 2009 the patient was referred to our department. An area of infected and exposed necrotic bone in the left mandible enclosed 1 dental implant. A panoramic radiograph and computed tomography scan showed an increased bone marrow density with peri-implant bone sequestration. The technetium Tc 99m scintigraphy–labeled granulocytes were positive for active bone infection. Bone exposure persisted for 8 weeks, and diagnosis of oral nitrogen-containing bisphosphonate (N-BP)–related osteonecrosis was made. On the basis of a review of the literature, this is the 10th case of BRONJ after implant placement in patients taking oral N-BPs. Despite the low risk of BRONJ occurrence after implant surgery in oral N-BP users, the fate of dental implants in these patients remains uncertain. Therefore patients at risk must be given a full explanation of the potential risks of implant failure and BRONJ development. Because the potential role of infection is still debated, great attention should be paid to the long-term oral hygiene of implant-prosthetic restorations.
Abstract
T cell infiltration and accumulation in pancreatic islets during autoimmune diabetes is largely dependent on TCR specificity for beta cell antigens. However, it is not fully clear whether ...the level of self-reactivity is an important determinant of T cell function in autoimmunity. We observed that CD5 expression is proportional to the level of self-reactivity based on Nur77-GFP reporter of TCR signaling and insulin tetramer staining of islet infiltrating T cells. Using RNAseq profiling, TCR sequencing, and cell transfer diabetes model we show that CD5 segregates functionally and transcriptionally distinct effector T cells. Transfer of CD5hi islet infiltrating T cells resulted in accelerated diabetes development compared to CD5lo cells (8 weeks vs. 11 weeks post transfer), suggesting that both T cell populations are able to re-infiltrate the pancreas and cause beta cell destruction, but CD5hi T cells exhibit increased pathogenicity. RNAseq analysis showed an increase in expression of Lag3 and FR4 in CD5hi cells, consistent with their increased activation and subsequent induction of regulatory and tolerogenic pathways. However, cell intrinsic negative regulators did not suppress IFNg production in CD5hi T cells, which was significantly higher compared to CD5lo cells. Our findings show that the strength of TCR reactivity to beta cell antigens is an important determinant of T cell pathogenicity in autoimmune diabetes.
Abstract
Type I Diabetes (T1D) is a T cell-mediated autoimmune disease that destroys insulin producing β cells found within the pancreatic islets of Langerhans. Insulin and Chromogranin A (ChgA) are ...the only currently known β cell antigens (βAg) necessary to initiate autoimmune diabetes in the non-obese diabetic (NOD) mice; however, expression of ChgA within medullary thymic epithelial cells (mTECs) has not yet been detected. Therefore, tolerance to ChgA may be due in part to peripheral dendritic cells (DCs) presenting ChgA to developing thymocytes and naïve T cells. For example, post-translational modification (PTM) of ChgA by fusion with an insulin peptide increases epitope immunogenicity by forming the neo-antigen, insulin-ChgA fusion peptide (2.5HIP). Pathogenic T cells that are specific for 2.5HIP, which is uniquely expressed in the pancreas, may escape thymic selection due to immunologic ignorance. The relative contribution of thymic and peripheral derived Ag in the selection of βAg specific CD4+ T cells and regulatory T cells (Tregs) remains unclear. To test the role of peripheral Ag exposure on thymic development of βAg specific Tregs, we generated a 50:50 mixed bone marrow chimera using NOD WT (CD45.2 × CD45.1) and BDC2.5 TCR Tg (CD45.1) bone marrow to study the role of peripheral DCs (CD45.2) on developing ChgA T cells. Next, we targeted thymic Langerin+ DCs with anti-Langerin linked to ChgA peptides in BDC2.5 TCR Tg mice. In both experiments, we found robust increase in the ratio and number of ChgA specific Tregs in the presence of the 2.5HIP, in adult and neonatal mice. The results suggest thymic exposure to PTM peptides by DCs enhances β cell specific Treg development and may alter T1D pathogenesis.
A nanocomposite adsorbent material was synthetized in order to obtain an efficient system able to remove, by means of chemical adsorption, mainly aromatic analytes dissolved in aqueous phase. The ...adsorbing substrate is composed by a magnetic responsive iron oxide (γ-Fe2O3) core coated by a shell of SiO2. The adsorption process is mediated by the chemical interaction among amine and silanol groups on the surface of the nanocomposite adsorbent and the analyte π cloud. The paramagnetic core allows to remove the formed nanosystem-analyte adduct quickly and completely, representing a huge improvement in comparison of the most used gravimetric separation. The removal efficiency obtained using the proposed nanocomposite reaches about 93% for the dangerous pollutant 1,2-phenylenediamine in water solution, 70% for dinitrophenol, 60% for aniline. Further, the nano-adsorbent was decorated with plasmonic Ag nanoparticles without significantly affecting the ability to adsorb the aromatic compounds. The presence of the plasmonic nanoparticles on the SiO2 surface allows to obtain surface enhanced Raman scattering (SERS) of the signals of the adsorbed aniline and 1,2-phenylenediamine proposing this approach to remove and, concomitantly, discriminate the retrieved pollutants from water.
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The new 2019 coronavirus 19 disease (CoVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses a serious threat to health systems. As a global health problem, this ...pandemic poses a huge threat to people and is responsible for significant morbidity and mortality worldwide. On the other hand, obesity has also reached epidemic proportions and poses another challenge to the healthcare system. There is increasing evidence of a strong association between obesity and CoVID-19 disease, but the mechanisms underlying the link between the two remain unclear and the role of obesity also remains to be elucidated. In particular obesity-related low-grade inflammation has been hypothesized as the Achille's heel that could predispose subjects with obesity to a more severe CoVID-19 compared to subjects with normal weight. Hence, we summarized recent evidence on the role of low-grade inflammation in clinical aspects of CoVID-19 in subjects with obesity in both childhood and adulthood. Further, we provide molecular insights to explain this link.
OBJECTIVES
This study aimed to evaluate the agreement between microscopic and molecular testing for differentiating feline intestinal bowel disease and small cell alimentary lymphoma in duodenal ...endoscopic biopsies.
METHODS
Four different diagnostic methods (cytology, histology, immunohistochemistry and clonality) were sequentially applied to 77 cases of feline chronic enteropathies. The agreement between the different diagnostic methods was calculated and survival data were obtained to assess the most reliable method for predicting outcome.
RESULTS
Seventy‐seven cases were included in the study. On multivariate survival analysis, only the clonality‐based diagnosis of lymphoma was significantly associated with poor survival, with a risk of enteropathy‐related death 2·8 times higher. By comparing the other tests with clonality, specificity was high (87 to 97%), whereas sensitivity was 36·8% for cytology, 39·5% for histology, 63·2% for immunohistochemistry, resulting in an overall accuracy of 62·3, 68·8 and 80·5%, respectively.
CLINICAL SIGNIFICANCE
Clonality analysis can consistently increase the possibility of correctly and early diagnosing small cell lymphoma on endoscopic biopsies. Histological suspicion of alimentary lymphoma, even if not confirmed by clonality, should never be ignored, as it may represent a debutant form of lymphoma or it may later progress to lymphoma.
In battery-powered medical instrumentation, the resolution and signal bandwidth of analog-to-digital converters (ADCs) have to be adapted to the needs of the application to avoid power wastage. This ...paper presents a reconfigurable successive approximation register (SAR) ADC implemented in 130 nm CMOS that resolves 5-14 bit with a maximum achievable effective number of bits (ENOB) of 13.5 using non-subtractive dither. In the proposed ADC design, the power consumption can be traded for accuracy to improve the energy efficiency and extend its application range, while reducing system integration complexity. A figure-of-merit (FoM) of 59 fJ/conversion is achieved at 1.2 V supply and the converter occupies an area of 0.42 mm 2 . Measurement results of the ADC integrated in a multi-channel analog front-end (AFE) circuit show the suitability of the ADC for portable medical monitoring devices.
The aim of this review is to provide the reader general and inspiring prospects on recent and promising fields of innovation in oral drug delivery. Nowadays, inventive drug delivery systems vary from ...geometrically modified and modular matrices, more close to “classic” pharmaceutical manufacturing processes, to futuristic bio micro-electro-mechanical systems (bioMEMS), based on manufacturing techniques borrowed from electronics and other fields. In these technologies new materials and creative solutions are essential designing intelligent drug delivery systems able to release the required drug at the proper body location with the correct release rate. In particular, oral drug delivery systems of the future are expected to have a significant impact on the treatment of diseases, such as AIDS, cancer, malaria, diabetes requiring complex and multi-drug therapies, as well as on the life of patients, whose age and/or health status make necessary a multiple pharmacological approach.
