New developments of pixel detectors based on GaAs sensors offer effective registration of the transition radiation (TR) X-rays and perform simultaneous measurements of their energies and emission ...angles. This unique feature opens new possibilities for particle identification on the basis of maximum available information about generated TR photons. Results of studies of TR energy-angular distributions using a 500 |j.m thick GaAs sensor attached to a Timepix3 chip are presented. Measurements, analysis techniques and a comparison with Monte Carlo (MC) simulations are described and discussed.
We are interested in the study of small RNAs (sRNAs) that over-accumulate during the plant hypersensitive response (HR), a form of programmed cell death that occurs in and around the site of ...infection when plants are challenged by pathogens. For this purpose, we have constructed, by subtractive hybridization, a cDNA library of
Arabidopsis sRNAs that are enriched during HR. Sequencing of randomly chosen clones provided evidence for the specific accumulation of several microRNAs as well as previously unidentified sRNAs. In a second approach, we have tested the possibility to hybridize labelled cDNAs derived from sRNAs to a DNA tiling array. We could reproducibly hybridize a custom-made tiling array covering
Arabidopsis chromosome 4, with small cDNAs as targets. Furthermore, we have found that the distribution of hybridized fragments with sRNAs extracted from control leaves is in good agreement with the abundance of
Arabidopsis sRNAs that correspond to this chromosome as determined by massive parallel sequence signature (MPSS).
PDF
