An inertial fusion implosion on the National Ignition Facility, conducted on August 8, 2021 (N210808), recently produced more than a megajoule of fusion yield and passed Lawson's criterion for ...ignition Phys. Rev. Lett. 129, 075001 (2022). Here we describe the experimental improvements that enabled N210808 and present the first experimental measurements from an igniting plasma in the laboratory. Ignition metrics like the product of hot-spot energy and pressure squared, in the absence of self-heating, increased by ~ 35%, leading to record values and an enhancement from previous experiments in the hot-spot energy (~ 3×), pressure (~ 2×), and mass (~ 2×). These results are consistent with self-heating dominating other power balance terms. The burn rate increases by an order of magnitude after peak compression, and the hot-spot conditions show clear evidence for burn propagation into the dense fuel surrounding the hot spot. These novel dynamics and thermodynamic properties have never been observed on prior inertial fusion experiments.
An indirect-drive inertial fusion experiment on the National Ignition Facility was driven using 2.05 MJ of laser light at a wavelength of 351 nm and produced 3.1±0.16 MJ of total fusion yield, ...producing a target gain G=1.5±0.1 exceeding unity for the first time in a laboratory experiment Phys. Rev. E 109, 025204 (2024)10.1103/PhysRevE.109.025204. Herein we describe the experimental evidence for the increased drive on the capsule using additional laser energy and control over known degradation mechanisms, which are critical to achieving high performance. Improved fuel compression relative to previous megajoule-yield experiments is observed. Novel signatures of the ignition and burn propagation to high yield can now be studied in the laboratory for the first time.
The change in the power balance, temporal dynamics, emission weighted size, temperature, mass, and areal density of inertially confined fusion plasmas have been quantified for experiments that reach ...target gains up to 0.72. It is observed that as the target gain rises, increased rates of self-heating initially overcome expansion power losses. This leads to reacting plasmas that reach peak fusion production at later times with increased size, temperature, mass and with lower emission weighted areal densities. Analytic models are consistent with the observations and inferences for how these quantities evolve as the rate of fusion self-heating, fusion yield, and target gain increase. At peak fusion production, it is found that as temperatures and target gains rise, the expansion power loss increases to a near constant ratio of the fusion self-heating power. Finally, this is consistent with models that indicate that the expansion losses dominate the dynamics in this regime.
Abstract
STUDY QUESTION
Does the upregulation of the zinc finger E-box binding homeobox 2 (ZEB2) transcription factor in human trophoblast cells lead to alterations in gene expression consistent with ...an epithelial–mesenchymal transition (EMT) and a consequent increase in invasiveness?
SUMMARY ANSWER
Overexpression of ZEB2 results in an epithelial–mesenchymal shift in gene expression accompanied by a substantial increase in the invasive capacity of human trophoblast cells.
WHAT IS KNOWN ALREADY
In-vivo results have shown that cytotrophoblast differentiation into extravillous trophoblast involves an epithelial–mesenchymal transition. The only EMT master regulatory factor which shows changes consistent with extravillous trophoblast EMT status and invasive capacity is the ZEB2 transcription factor.
STUDY DESIGN, SIZE, DURATION
This study is a mechanistic investigation of the role of ZEB2 in trophoblast differentiation. We generated stable ZEB2 overexpression clones using the epithelial BeWo and JEG3 choriocarcinoma lines. Using these clones, we investigated the effects of ZEB2 overexpression on the expression of EMT-associated genes and proteins, cell morphology and invasive capability.
PARTICIPANTS/MATERIALS, SETTING, METHODS
We used lentiviral transduction to overexpress ZEB2 in BeWo and JEG3 cells. Stable clones were selected based on ZEB2 expression and morphology. A PCR array of EMT-associated genes was used to probe gene expression. Protein measurements were performed by western blotting. Gain-of-function was assessed by quantitatively measuring cell invasion rates using a Transwell assay, a 3D bioprinted placenta model and the xCelligenceTM platform.
MAIN RESULTS AND THE ROLE OF CHANCE
The four selected clones (2 × BeWo, 2 × JEG3, based on ZEB2 expression and morphology) all showed gene expression changes indicative of an EMT. The two clones (1 × BeWo, 1 × JEG3) showing >40-fold increase in ZEB2 expression also displayed increased ZEB2 protein; the others, with increases in ZEB2 expression <14-fold did not. The two high ZEB2-expressing clones demonstrated robust increases in invasive capacity, as assessed by three types of invasion assay. These data identify ZEB2-mediated transcription as a key mechanism transforming the epithelial-like trophoblast into cells with a mesenchymal, invasive phenotype.
LARGE SCALE DATA
PCR array data have been deposited in the GEO database under accession number GSE116532.
LIMITATIONS, REASONS FOR CAUTION
These are in-vitro studies using choriocarcinoma cells and so the results should be interpreted in view of these limitations. Nevertheless, the data are consistent with in-vivo findings and are replicated in two different cell lines.
WIDER IMPLICATIONS OF THE FINDINGS
The combination of these data with the in-vivo findings clearly identify ZEB2-mediated EMT as the mechanism for cytotrophoblast differentiation into extravillous trophoblast. Having characterized these cellular mechanisms, it will now be possible to identify the intracellular and extracellular regulatory components which control ZEB2 and trophoblast differentiation. It will also be possible to identify the aberrant factors which alter differentiation in invasive pathologies such as preeclampsia and abnormally invasive placenta (AKA accreta, increta, percreta).
STUDY FUNDING AND COMPETING INTEREST(s)
Funding was provided by the Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine and Surgery at Hackensack Meridian Health, Hackensack, NJ. The 3D bioprinted placental model work done in Drs Kim and Fisher’s labs was supported by the Children’s National Medical Center. The xCELLigence work done in Dr Birge’s lab was supported by NIH CA165077. The authors declare no competing interests.
We report the first observation of the parity-violating gamma-ray asymmetry A_{γ}^{np} in neutron-proton capture using polarized cold neutrons incident on a liquid parahydrogen target at the ...Spallation Neutron Source at Oak Ridge National Laboratory. A_{γ}^{np} isolates the ΔI=1, ^{3}S_{1}→^{3}P_{1} component of the weak nucleon-nucleon interaction, which is dominated by pion exchange and can be directly related to a single coupling constant in either the DDH meson exchange model or pionless effective field theory. We measured A_{γ}^{np}=-3.0±1.4(stat)±0.2(syst)×10^{-8}, which implies a DDH weak πNN coupling of h_{π}^{1}=2.6±1.2(stat)±0.2(syst)×10^{-7} and a pionless EFT constant of C^{^{3}S_{1}→^{3}P_{1}}/C_{0}=-7.4±3.5(stat)±0.5(syst)×10^{-11} MeV^{-1}. We describe the experiment, data analysis, systematic uncertainties, and implications of the result.
We study current-driven magnetization switching in nanofabricated Ni(84)Fe(16)/Cu/Ni(84)Fe16 trilayers at 295 and 4.2 K. The shape of the hysteretic switching diagram at low magnetic field changes ...with temperature. The reversible behavior at higher fields involves two phenomena, a threshold current for magnetic excitations closely correlated with the switching current, and a peak in differential resistance characterized by telegraph noise, with an average period that decreases exponentially with current and shifts with temperature. We interpret both static and dynamic results at 295 and 4.2 K in terms of thermal activation over a potential barrier, with a current-dependent effective magnetic temperature.
Landscape of two-proton radioactivity Olsen, E; Pfützner, M; Birge, N ...
Physical review letters,
2013-May-31, Letnik:
110, Številka:
22
Journal Article
Recenzirano
Odprti dostop
Ground-state two-proton (2p) radioactivity is a decay mode found in isotopes of elements with even atomic numbers located beyond the two-proton drip line. So far, this exotic process has been ...experimentally observed in a few light- and medium-mass nuclides with Z≤30. In this study, using state-of-the-art nuclear density functional theory, we globally analyze 2p radioactivity and for the first time identify 2p-decay candidates in elements heavier than strontium. We predict a few cases where the competition between 2p emission and α decay may be observed. In nuclei above lead, the α-decay mode is found to be dominating and no measurable candidates for the 2p radioactivity are expected.
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