Oak (Quercus petraea and Quercus robur), an emblematic species in French forests, has a strong patrimonial value and plays a leading role in the forestry and forest-based industry. However, the ...industry appears to be confronted with major problems (e.g. supply to markets). We tried to better understand and analyse those problems by studying the oak chain in north eastern France (Alsace, Franche-Comté, Lorraine) through interviews conducted with public forest managers and players involved in primary processing. In order to survey a broad range of points of view and have a basis for comparison, the study area also included two Landers in south western Germany (Baden-Wurttemberg, Rhineland-Palatinate). A qualitative analysis of 25 interviews conducted on both sides of the Franco-German border enabled us to identify contrasting views and points of consensus about the issues relating to forest resources, the perception of the sector by the players and the entrepreneurial dynamic. Possible future developments could also be distinguished, some of which are already discussed in the literature while others were particular to this survey.
Le Chêne (Quercus petraea et Quercus robur), essence emblématique de la forêt française, a une valeur patrimoniale forte et joue un rôle majeur dans la filière forêt-bois. Néanmoins, cette filière semble être confrontée à des problèmes importants (par exemple au niveau de l’approvisionnement et des marchés) que nous avons cherché à mieux comprendre et à analyser. Notre étude s’intéresse plus particulièrement à la filière Chêne dans le nord-est de la France (Alsace, Franche-Comté, Lorraine) et se base sur des entretiens réalisés auprès de gestionnaires forestiers publics et d’acteurs de la première transformation. Afin d’obtenir une gamme large de points de vue et de disposer d’éléments de comparaison, la zone d’étude intègre également deux Länder du sud-ouest de l’Allemagne (Bade-Wurtemberg, Rhénanie-Palatinat). L’analyse qualitative de 25 entretiens, réalisés de part et d’autre de la frontière franco-allemande, a permis de dégager des avis divergents et des points de consensus au sujet de thématiques relatives à la ressource forestière, à la perception de la filière par les acteurs et aux dynamiques d’entreprise. Des perspectives d’évolution ont aussi pu être distinguées, comprenant des réflexions déjà évoquées dans la littérature et d’autres plus personnelles à cette étude.
Antibiotics (ABX) compromise the efficacy of programmed cell death protein 1 (PD-1) blockade in cancer patients, but the mechanisms underlying their immunosuppressive effects remain unknown. By ...inducing the down-regulation of mucosal addressin cell adhesion molecule 1 (MAdCAM-1) in the ileum, post-ABX gut recolonization by Enterocloster species drove the emigration of enterotropic α4β7 + CD4 + regulatory T 17 cells into the tumor. These deleterious ABX effects were mimicked by oral gavage of Enterocloster species, by genetic deficiency, or by antibody-mediated neutralization of MAdCAM-1 and its receptor, α4β7 integrin. By contrast, fecal microbiota transplantation or interleukin-17A neutralization prevented ABX-induced immunosuppression. In independent lung, kidney, and bladder cancer patient cohorts, low serum levels of soluble MAdCAM-1 had a negative prognostic impact. Thus, the MAdCAM-1–α4β7 axis constitutes an actionable gut immune checkpoint in cancer immunosurveillance.
Tumor spheroids are promising three-dimensional (3D) in vitro tumor models for the evaluation of drug delivery methods. The design of noninvasive and targeted drug methods is required to improve the ...intratumoral bioavailability of chemotherapeutic drugs and reduce their adverse off-target effects. Among such methods, microbubble-assisted ultrasound (MB-assisted US) is an innovative modality for noninvasive targeted drug delivery. The aim of the present study is to evaluate the efficacy of this US modality for the delivery of bleomycin, doxorubicin, and irinotecan in colorectal cancer (CRC) spheroids. MB-assisted US permeabilized the CRC spheroids to propidium iodide, which was used as a drug model without affecting their growth and viability. Histological analysis and electron microscopy revealed that MB-assisted US affected only the peripheral layer of the CRC spheroids. The acoustically mediated bleomycin delivery induced a significant decrease in CRC spheroid growth in comparison to spheroids treated with bleomycin alone. However, this US modality did not improve the therapeutic efficacy of doxorubicin and irinotecan on CRC spheroids. In conclusion, this study demonstrates that tumor spheroids are a relevant approach to evaluate the efficacy of MB-assisted US for the delivery of chemotherapeutics.
BackgroundProstate cancer (PC) responds to androgen deprivation therapy (ADT) usually in a transient fashion, progressing from hormone-sensitive PC (HSPC) to castration-resistant PC (CRPC). We ...investigated a mouse model of PC as well as specimens from PC patients to unravel an unsuspected contribution of thymus-derived T lymphocytes and the intestinal microbiota in the efficacy of ADT.MethodsPreclinical experiments were performed in PC-bearing mice, immunocompetent or immunodeficient. In parallel, we prospectively included 65 HSPC and CRPC patients (Oncobiotic trial) to analyze their feces and blood specimens.ResultsIn PC-bearing mice, ADT increased thymic cellularity and output. PC implanted in T lymphocyte-depleted or athymic mice responded less efficiently to ADT than in immunocompetent mice. Moreover, depletion of the intestinal microbiota by oral antibiotics reduced the efficacy of ADT. PC reduced the relative abundance of Akkermansia muciniphila in the gut, and this effect was reversed by ADT. Moreover, cohousing of PC-bearing mice with tumor-free mice or oral gavage with Akkermansia improved the efficacy of ADT. This appears to be applicable to PC patients because long-term ADT resulted in an increase of thymic output, as demonstrated by an increase in circulating recent thymic emigrant cells (sjTRECs). Moreover, as compared with HSPC controls, CRPC patients demonstrated a shift in their intestinal microbiota that significantly correlated with sjTRECs. While feces from healthy volunteers restored ADT efficacy, feces from PC patients failed to do so.ConclusionsThese findings suggest the potential clinical utility of reversing intestinal dysbiosis and repairing acquired immune defects in PC patients.
•Largest French series on HDD brachytherapy boost for prostate cancer.•The quality of the data collection is one of its strengths.•This study shows the efficacy of HDD brachytherapy boost in ...intermediate and high risk prostate cancers at the national level and a homogeneity of practices in France.
Radiotherapy dose escalation improves biochemical control in intermediate- or high-risk prostate cancer. Brachytherapy boost was shown to further improve biochemical control compared to radiotherapy alone in three randomized trials. The SFRO brachytherapy group sought to evaluate the efficacy and toxicity of BT-boost for intermediate and high-risk prostate cancer in real life, and to determine prognostic factors for efficacy and toxicity.
A retrospective study was conducted, including all patients with intermediate- or high-risk prostate cancer treated with a combination of external beam radiotherapy (EBRT) and high dose-rate brachytherapy boost (HDR-BB), from 2006 until December 2019 at two centers. Patient characteristics, initial disease, treatment and follow-up were collected.
709 patients from two centers were analyzed given a short follow-up in the other centers. Out of those, 277 were intermediate risk (170 favorable and 107 unfavorable) and 432 were high risk. The median EBRT and HDR-BB doses were 46 Gy (35–50) and 14 Gy (10–20). After a median follow-up of 62 months, biochemical control at 5 years was 87.5 % for the overall population, 91 % and 85 % for intermediate- and high-risk cancers, respectively. At 5 years, biochemical and clinical relapse-free survival, metastasis-free survival and local control rates were 83 %, 90 % and 97 % respectively. 5-years overall survival was 94 %. Late grade 2 or higher GU or GI toxicity was found in 36 patients (5 %) and 9 patients (1.3 %).
This bicenter analysis shows the efficacy and tolerability of HDR-BB as a complement to external radiotherapy. Further improvements such as combination with new hormonal agents or new brachytherapy-radiotherapy fractionation regimens are warranted to improve further the outcomes and therapeutic ratio.
Considérant les pratiques langagières comme partie intégrante des pratiques migratoires, ce dossier s’intéresse aux mises en mots de la mort et de la violence aux frontières dans le cadre des migr...
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