41.
Dostopno za:
UL
42.
Involvement of Smads in TGFβ1-induced furin (fur) transcription
Blanchette, François; Rudd, Penny; Grondin, Francine ...
Journal of cellular physiology,
August 2001, Letnik:
188, Številka:
2
Journal Article
Furin is recognized as being one of the main convertases of the cellular constitutive secretion pathway but the mechanisms regulating its expression are still unknown. We have previously demonstrated ...
that TGFβ1 up‐regulates its own converting enzyme, furin, creating a novel activation/regulation cycle of potential importance in a variety of physiological and pathophysiological conditions. The fur (fes upstream region) gene is regulated via three alternative promoters; P1, P1A, and P1B. To gain insight into the molecular mechanism(s) underlying this up‐regulation, we performed transient cell transfections with P1, P1A, and P1B promoter luciferase constructs. Transfection experiments in HepG2 cells revealed that fur P1 promoter is the strongest and the most sensitive to TGFβ1 stimulation (5 ng/ml) (3.2‐fold vs. 2.4‐fold for P1A and 2.1‐fold for P1B). Cotransfection with either a dominant negative mutant form of Smad2 Smad2(3SA) or a known Smad inhibitor Smad7 inhibit constitutive and TGFβ1‐induced luciferase activity indicating the participation of endogenous Smads. Increased levels of TGFβ1‐induced transcriptional activation of the P1 promoter by overexpression of Smad2 and/or Smad4 is greatly reduced in the presence of Smad2(3SA) and completely inhibited by Smad7, suggesting the participation of endogenous Smad2/Smad4 complexes. Furthermore, the fork‐head activin signal transducer (FAST‐1), known to interact with Smad2/Smad4 complexes, is a potent stimulator of TGFβ1‐induced transactivation of the fur P1 promoter. Five prime‐deletion analysis of this promoter identified the proximal region (between positions −8734 and −7925), as the nucleotide stretch that carries most of the transcriptional activation of fur P1 promoter by Smad2. Overall, the present data demonstrate that Smad2 and Smad4 possibly in complex with FAST‐1 or other DNA binding partners participate in the constitutive and inducible transactivation of the fur P1 promoter. This represents the first detailed study of the transcriptional regulation of the fur gene. © 2001 Wiley‐Liss, Inc.
več
Dostopno za:
BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
43.
44.
Written Proof
Blanchette, Jean-François
Burdens of Proof,
2012, 20120427
Book Chapter
This chapter contains sections titled: Contracts, To Reform or Not to Reform, Global Signatures, La Loi du 13 mars 2000, Conclusion
Dostopno za:
UPUK
45.
On the Brink of a Revolution
Blanchette, Jean-François
Burdens of Proof,
2012, 20120427
Book Chapter
This chapter contains sections titled: Decentralized Cryptography, Cryptography and National Security, Cryptographic Research Programs, Conclusion
Dostopno za:
UPUK
46.
The Cryptographic Imagination
Blanchette, Jean-François
Burdens of Proof,
2012, 20120427
Book Chapter
This chapter contains sections titled: The Random Oracle Model, Memory, Perception, Material Objects, Cognition, Conclusion
Dostopno za:
UPUK
47.
The Equivalent of a Written Signature
Blanchette, Jean-François
Burdens of Proof,
2012, 20120427
Book Chapter
This chapter contains sections titled: The Early History, Security Services, Attacks and Adversaries, Mutations, Mathematics and the Real World, Conclusion
Dostopno za:
UPUK
48.
Paper and State
Blanchette, Jean-François
Burdens of Proof,
2012, 20120427
Book Chapter
This chapter contains sections titled: Dematerialized Notaries, Records of Civil Status, The Land Registry of Alsace-Moselle, Conclusion
Dostopno za:
UPUK
49.
Dostopno za:
UPUK
50.
CONCLUSION
Blanchette, Jean-François
Du Coq à L'âme,
02/2014
Book Chapter
Le premier Festival de sculptures en art populaire de Saint-Ulric de Matane tenu en août 2013 est un bel exemple de la vivacité de l’art populaire. Mais où nous mène l’art populaire? Bien malin qui ...
pourrait le dire. Car, si l’art a une direction, c’est celle de l’inconnu, de la découverte, de l’expérience nouvelle, de l’imagination qui vise parfois à séduire, mais qui ne sert, le plus souvent, qu’à s’exprimer. Car créer est une manière de vivre. Pour l’artiste, la vie n’existe pas sans la création.
Comme l’art populaire est l’art de la culture populaire, il faut rechercher les
več
Dostopno za:
NUK, UL, UM, UPUK
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