Point and regional centromeres specify a unique site on each chromosome for kinetochore assembly. The point centromere in budding yeast is a unique 150-bp DNA sequence, which supports a kinetochore ...with only one microtubule attachment. In contrast, regional centromeres are complex in architecture, can be up to 5 Mb in length, and typically support many kinetochore-microtubule attachments. We used quantitative fluorescence microscopy to count the number of core structural kinetochore protein complexes at the regional centromeres in fission yeast and Candida albicans. We find that the number of CENP-A nucleosomes at these centromeres reflects the number of kinetochore-microtubule attachments instead of their length. The numbers of kinetochore protein complexes per microtubule attachment are nearly identical to the numbers in a budding yeast kinetochore. These findings reveal that kinetochores with multiple microtubule attachments are mainly built by repeating a conserved structural subunit that is equivalent to a single microtubule attachment site.
This is the first of a series of papers aimed at characterizing the populations detected in the high-latitude sky of the Fermi-LAT survey. In this work, we focus on the intrinsic spectral and flux ...properties of the source sample. We show that when selection effects are properly taken into account, Fermi sources are on average steeper than previously found (e.g., in the bright source list) with an average photon index of 2.40 +/- 0.02 over the entire 0.1-100 GeV energy band. We confirm that flat spectrum radio quasars have steeper spectra than BL Lacertae objects with an average index of 2.48 +/- 0.02 versus 2.18 +/- 0.02. Using several methods, we build the deepest source count distribution at GeV energies, deriving that the intrinsic source (i.e., blazar) surface density at F-100 >= 10(-9) ph cm(2) s(-1) is 0.12(-0.02)(+0.03) deg(-2). The integration of the source count distribution yields that point sources contribute 16(+/- 1.8)% (+/- 7% systematic uncertainty) of the GeV isotropic diffuse background. At the fluxes currently reached by LAT, we can rule out the hypothesis that pointlike sources (i.e., blazars) produce a larger fraction of the diffuse emission.
The kinetochore is a macromolecular protein machine 1 that links centromeric chromatin to the plus ends of one or more microtubules (MTs) and segregates chromosomes during cell division. Its core ...structure consists of eight multicomponent protein complexes, most of which are conserved in all eukaryotes. We use an in vivo two-color fluorescence microscopy technique to determine, for the first time, the location of these proteins along the budding yeast kinetochore axis at nanometer resolution. Together with kinetochore protein counts 2, 3, these localizations predict the 3D protein architecture of a metaphase kinetochore-microtubule attachment and provide new functional insights. We also find that the kinetochore becomes much shorter in anaphase as metaphase tension is lost. Shortening is due mainly to a decrease in the length of the Ndc80 complex, which may result either from intramolecular bending of the Ndc80 complex at the kink within the stalk region of the Ndc80-Nuf2 dimer 4, 5 or from a change in its orientation relative to the microtubule axis. Conformational changes within the Ndc80 and Mtw1 complexes may serve as mechanical cues for tension-dependent regulation of MT attachment and the spindle-assembly checkpoint. The geometry of the core structure of the budding yeast kinetochore reported here is remarkably similar to that found in mammalian kinetochores, indicating that kinetochore structure is conserved in eukaryotes with either point or regional centromeres.
The gamma-ray energy spectra of bright blazars of the LAT Bright AGN Sample LBAS) are investigated using Fermi-LAT data. Spectral properties hardness, curvature, and variability) established using a ...data set accumulated over 6 months of operation are presented and discussed for different blazar classes and subclasses: flat spectrum radio quasars (FSRQs), low-synchrotron peaked BLLacs (LSP-BLLacs), intermediate-synchrotron peaked BLLacs (ISP-BLLacs), and high-synchrotron peaked BLLacs (HSP-BLLacs). The distribution of photon index G, obtained from a power-law fit above 100 MeV) is found to correlate strongly with blazar subclass. The change in spectral index from that averaged over the 6 months observing period is < 0.2-0.3 when the flux varies by about an order of magnitude, with a tendency toward harder spectra when the flux is brighter for FSRQs and LSP-BLLacs. A strong departure from a single power-law spectrum appears to be a common feature for FSRQs. This feature is also present for some high-luminosity LSP-BLLacs, and a small number of ISP-BLLacs. It is absent in all LBAS HSP-BLLacs. For 3C 454.3 and AO 0235+164, the two brightest FSRQ source and LSP-BLLac source, respectively, a broken power law (BPL) gives the most acceptable of power law, BPL, and curved forms. The consequences of these findings are discussed.
We present multiwavelength studies of the 106.6 ms gamma-ray pulsar PSR J1907+06 near the TeV source MGRO J1908+06. Timing observations with Fermi result in a precise position determination for the ...pulsar of R.A. = 19h07m547(2), decl. = +06:02:16(2) placing the pulsar firmly within the TeV source extent, suggesting the TeV source is the pulsar wind nebula of PSR J1907+0602. Pulsed gamma-ray emission is clearly visible at energies from 100 MeV to above 10 GeV. The phase-averaged power-law index in the energy range E > 0.1 GeV is = 1.76 \pm 0.05 with an exponential cutoff energy E_{c} = 3.6 \pm 0.5 GeV. We present the energy-dependent gamma-ray pulsed light curve as well as limits on off-pulse emission associated with the TeV source. We also report the detection of very faint (flux density of ~3.4 microJy) radio pulsations with the Arecibo telescope at 1.5 GHz having a dispersion measure DM = 82.1 \pm 1.1 cm^{-3}pc. This indicates a distance of 3.2 \pm 0.6 kpc and a pseudo-luminosity of L_{1400} ~ 0.035 mJy kpc^2. A Chandra ACIS observation revealed an absorbed, possibly extended, compact <(4 arcsec) X-ray source with significant non-thermal emission at R.A. = 19h07m54.76, decl. = +06:02:14.6 with a flux of 2.3^{+0.6}_{-1.4} X 10^{-14} erg cm^{-2} s^{-1}. From archival ASCA observations, we place upper limits on any arcminute scale 2--10 keV X-ray emission of ~ 1 X 10^{-13} erg cm^{-2} s^{-1}. The implied distance to the pulsar is compatible with that of the supernova remnant G40.5-0.5, located on the far side of the TeV nebula from PSR J1907+0602, and the S74 molecular cloud on the nearer side which we discuss as potential birth sites.
ABSTRACT RCW 86 is a young supernova remnant (SNR) showing a shell-type structure at several wavelengths and is thought to be an efficient cosmic-ray (CR) accelerator. Earlier Fermi Large Area ...Telescope results reported the detection of γ-ray emission coincident with the position of RCW 86 but its origin (leptonic or hadronic) remained unclear due to the poor statistics. Thanks to 6.5 years of data acquired by the Fermi-LAT and the new event reconstruction Pass 8, we report the significant detection of spatially extended emission coming from RCW 86. The spectrum is described by a power-law function with a very hard photon index ( ) in the 0.1-500 GeV range and an energy flux above 100 MeV of ( ) erg cm−2 s−1. Gathering all the available multiwavelength (MWL) data, we perform a broadband modeling of the nonthermal emission of RCW 86 to constrain parameters of the nearby medium and bring new hints about the origin of the γ-ray emission. For the whole SNR, the modeling favors a leptonic scenario in the framework of a two-zone model with an average magnetic field of 10.2 0.7 G and a limit on the maximum energy injected into protons of 2 × 1049 erg for a density of 1 cm−3. In addition, parameter values are derived for the north-east and south-west (SW) regions of RCW 86, providing the first indication of a higher magnetic field in the SW region.
We present gamma-ray observations with the LAT on board the Fermi Gamma-Ray Telescope of the nearby radio galaxy Centaurus~A. The previous EGRET detection is confirmed, and the localization is ...improved using data from the first 10 months of Fermi science operation. In previous work, we presented the detection of the lobes by the LAT; in this work, we concentrate on the gamma-ray core of Cen~A. Flux levels as seen by the LAT are not significantly different from that found by EGRET, nor is the extremely soft LAT spectrum ($\G=2.67\pm0.10_{stat}\pm0.08_{sys}$ where the photon flux is $\Phi\propto E^{-\G}$). The LAT core spectrum, extrapolated to higher energies, is marginally consistent with the non-simultaneous HESS spectrum of the source. The LAT observations are complemented by simultaneous observations from Suzaku, the Swift Burst Alert Telescope and X-ray Telescope, and radio observations with the Tracking Active Galactic Nuclei with Austral Milliarcsecond Interferometry (TANAMI) program, along with a variety of non-simultaneous archival data from a variety of instruments and wavelengths to produce a spectral energy distribution (SED). We fit this broadband data set with a single-zone synchrotron/synchrotron self-Compton model, which describes the radio through GeV emission well, but fails to account for the non-simultaneous higher energy TeV emission observed by HESS from 2004-2008. The fit requires a low Doppler factor, in contrast to BL Lacs which generally require larger values to fit their broadband SEDs. This indicates the $\g$-ray emission originates from a slower region than that from BL Lacs, consistent with previous modeling results from Cen~A. This slower region could be a slower moving layer around a fast spine, or a slower region farther out from the black hole in a decelerating flow.
We report the Fermi Large Area Telescope (LAT) discovery of high-energy (MeV/GeV) Delta *g-ray emission positionally consistent with the center of the radio galaxy M87, at a source significance of ...over 10 Delta *s in 10 months of all-sky survey data. Following the detections of Cen A and Per A, this makes M87 the third radio galaxy seen with the LAT. The faint point-like Delta *g-ray source has a >100 MeV flux of 2.45 (+/-0.63) X 10-8 photons cm-2 s-1 (photon index = 2.26 +/- 0.13) with no significant variability detected within the LAT observation. This flux is comparable with the previous EGRET upper limit (<2.18 X 10-8 photons cm-2 s-1, 2 Delta *s), thus there is no evidence for a significant MeV/GeV flare on decade timescales. Contemporaneous Chandra and Very Long Baseline Array data indicate low activity in the unresolved X-ray and radio core relative to previous observations, suggesting M87 is in a quiescent overall level over the first year of Fermi-LAT observations. The LAT Delta *g-ray spectrum is modeled as synchrotron self-Compton (SSC) emission from the electron population producing the radio-to-X-ray emission in the core. The resultant SSC spectrum extrapolates smoothly from the LAT band to the historical-minimum TeV emission. Alternative models for the core and possible contributions from the kiloparsec-scale jet in M87 are considered, and cannot be excluded.
We present the analysis of the interstellar gamma-ray emission measured by the Fermi Large Area Telescope toward a region in the second Galactic quadrant at 100 degrees <= l <= 145 degrees and ...-15 degrees <= b <= +30 degrees. This region encompasses the prominent Gould Belt clouds of Cassiopeia, Cepheus, and the Polaris flare, as well as atomic and molecular complexes at larger distances, like that associated with NGC 7538 in the Perseus arm. The good kinematic separation in velocity between the local, Perseus, and outer arms, and the presence of massive complexes in each of them, make this region well suited to probe cosmic rays (CRs) and the interstellar medium beyond the solar circle. The gamma-ray emissivity spectrum of the gas in the Gould Belt is consistent with expectations based on the locally measured CR spectra. The gamma-ray emissivity decreases from the Gould Belt to the Perseus arm, but the measured gradient is flatter than expectations for CR sources peaking in the inner Galaxy as suggested by pulsars. The X-CO = N(H-2)/W-CO conversion factor is found to increase from (0.87 +/- 0.05) x 10(20) cm(-2) (K km s(-1))(-1) in the Gould Belt to (1.9 +/- 0.2) x 10(20) cm(-2) (K km s(-1))(-1) in the Perseus arm. We derive masses for the molecular clouds under study. Dark gas, not properly traced by radio and microwave surveys, is detected in the Gould Belt through a correlated excess of dust and gamma-ray emission: its mass amounts to similar to 50% of the CO-traced mass.
ABSTRACT
We report observation of the supernova remnant (SNR) IC 443 (G189.1+3.0) with the
Fermi Gamma-ray Space Telescope
Large Area Telescope (LAT) in the energy band between 200 MeV and 50 GeV. ...IC 443 is a shell-type SNR with mixed morphology located off the outer Galactic plane where high-energy emission has been detected in the X-ray, GeV and TeV gamma-ray bands. Past observations suggest IC 443 has been interacting with surrounding interstellar matter. Proximity between dense shocked molecular clouds and GeV–TeV gamma-ray emission regions detected by
EGRET
,
MAGIC
, and
VERITAS
suggests an interpretation that cosmic-ray (CR) particles are accelerated by the SNR. With the high gamma-ray statistics and broad energy coverage provided by the LAT, we accurately characterize the gamma-ray emission produced by the CRs accelerated at IC 443. The emission region is extended in the energy band with θ
68
= 0.°27 ± 0.°01(stat) ± 0.°03(sys) for an assumed two-dimensional Gaussian profile and overlaps almost completely with the extended source region of
VERITAS
. Its centroid is displaced significantly from the known pulsar wind nebula (PWN) which suggests the PWN is not the major contributor in the present energy band. The observed spectrum changes its power-law slope continuously and continues smoothly to the
MAGIC
and
VERITAS
data points. The combined gamma-ray spectrum (200 MeV <
E
< 2 TeV) is reproduced well by decays of neutral pions produced by a broken power-law proton spectrum with a break around 70 GeV.
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