Cell-free DNA (cfDNA) analysis is a minimally invasive method that can be used to detect genomic abnormalities by directly testing a blood sample. This method is particularly useful for ...immunosuppressed patients, who are at high risk of complications from tissue biopsy. The cfDNA tumor fraction (TF) varies greatly across cancer type and between patients. Thus, the detection of molecular alterations is highly dependent on the circulating TF. In our study, we aimed to calculate the TF and characterize the copy number aberration (CNA) profile of cfDNA from patients with rare malignancies occurring in immunosuppressed environments or immune-privileged sites. To accomplish this, we recruited 36 patients: 19 patients with non-Hodgkin lymphoma (NHL) who were either human immunodeficiency virus (HIV)-positive or organ transplant recipients, 5 HIV-positive lung cancer patients, and 12 patients with glioma. cfDNA was extracted from the patients’ plasma and sequenced using low-coverage whole genome sequencing (LC-WGS). The cfDNA TF was then calculated using the ichorCNA bioinformatic algorithm, based on the CNA profile. In parallel, we performed whole exome sequencing of patient tumor tissue and cfDNA samples with detectable TFs. We detected a cfDNA TF in 29% of immune-suppressed patients (one patient with lung cancer and six with systemic NHL), with a TF range from 8 to 70%. In these patients, the events detected in the CNA profile of cfDNA are well-known events associated with NHL and lung cancer. Moreover, cfDNA CNA profile correlated with the CNA profile of matched tumor tissue. No tumor-derived cfDNA was detected in the glioma patients. Our study shows that tumor genetic content is detectable in cfDNA from immunosuppressed patients with advanced NHL or lung cancer. LC-WGS is a time- and cost-effective method that can help select an appropriate strategy for performing extensive molecular analysis of cfDNA. This technique also enables characterization of CNAs in cfDNA when sufficient tumor content is available. Hence, this approach can be used to collect useful molecular information that is relevant to patient care.
Cell-free DNA (cfDNA) analysis is a minimally invasive method that can be used to detect genomic abnormalities by directly testing a blood sample. This method is particularly useful for ...immunosuppressed patients, who are at high risk of complications from tissue biopsy. The cfDNA tumor fraction (TF) varies greatly across cancer type and between patients. Thus, the detection of molecular alterations is highly dependent on the circulating TF. In our study, we aimed to calculate the TF and characterize the copy number aberration (CNA) profile of cfDNA from patients with rare malignancies occurring in immunosuppressed environments or immune-privileged sites. To accomplish this, we recruited 36 patients: 19 patients with non-Hodgkin lymphoma (NHL) who were either human immunodeficiency virus (HIV)-positive or organ transplant recipients, 5 HIV-positive lung cancer patients, and 12 patients with glioma. cfDNA was extracted from the patients’ plasma and sequenced using low-coverage whole genome sequencing (LC-WGS). The cfDNA TF was then calculated using the ichorCNA bioinformatic algorithm, based on the CNA profile. In parallel, we performed whole exome sequencing of patient tumor tissue and cfDNA samples with detectable TFs. We detected a cfDNA TF in 29% of immune-suppressed patients (one patient with lung cancer and six with systemic NHL), with a TF range from 8 to 70%. In these patients, the events detected in the CNA profile of cfDNA are well-known events associated with NHL and lung cancer. Moreover, cfDNA CNA profile correlated with the CNA profile of matched tumor tissue. No tumor-derived cfDNA was detected in the glioma patients. Our study shows that tumor genetic content is detectable in cfDNA from immunosuppressed patients with advanced NHL or lung cancer. LC-WGS is a time- and cost-effective method that can help select an appropriate strategy for performing extensive molecular analysis of cfDNA. This technique also enables characterization of CNAs in cfDNA when sufficient tumor content is available. Hence, this approach can be used to collect useful molecular information that is relevant to patient care.
Undoped and Dy3+ (0.25, 0.5, 0.8 and 1.5at.%) doped ZnO were elaborated by solid-state reaction method. The ZnO:Dy3+ samples were characterized using X-ray diffraction (XRD), Raman spectroscopy, ...UV–vis diffuse reflectance spectroscopy and photoluminescence (PL). The XRD analysis confirms the wurtzite structure of ZnO. A slight shift to lower angles, of the (101) peak, is seen with Dy3+ content, indicating the substitution of these ions into the ZnO lattice. Raman study indicates the good crystallinity of all ZnO:Dy3+ samples and confirms the substitution of Zn2+ by Dy3+. The band gap energy was found to increase then decrease with Dy content. The PL excitation spectra (PLE) of Dy3+ showed six excitation bands with hypersensitive at 346nm (6H15/2→6P7/2). PL spectra show principally three emission bands relatives to 4F9/2→6H15/2 (476nm), 4F9/2→6H13/2 (567nm) and 4F9/2→6H11/2 (658nm) transitions. The concentration dependency of PL intensity indicates a quenching for Dy3+ concentration above 0.5at.%. The PL lifetime of 4F9/2 metastable state was measured and discussed for all Dy content in ZnO. The temperature dependency of PL intensity is investigated for ZnO:Dy (0.5%) sample and the activation energy is determined. The CIE chromaticity color coordinate shows that ZnO:Dy3+ can be useful for white luminescence applications.
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patients with chronic kidney disease commonly exhibit testosterone deficiency. We aimed through the current study to assess the prevalence and the risk factors of hypogonadism in male patients on ...hemodialysis and to establish their relationship with erectile dysfunction.
we conducted a cross-sectional study based on data collected from hemodialysis male patients. Sociodemographic and clinical data as well as hormone levels were collected from January 2017 to December 2017. Sex hormones were measured in all subjects. The International Index of Erectile Function was used to evaluate erectile dysfunction. Data were expressed as mean ± standard deviation, and frequencies (number), and proportions (%).
one hundred and ten: 55 male hemodialysis patients were recruited. The level of follicule-stimulating hormone, luteinizing hormone and prolactin were high and the level of testosterone was low in the hemodialysis group. Hypogonadism was significantly linked to advanced age, anemia, and absence of treatment by erythropoietin. The incidence of erectile dysfunction was high and the erectile function score was low. Testosterone significantly dropped in patients with erectile dysfunction.
hypogonadism was so prevalent in the hemodialysis men and it was associated with erectile dysfunction. Future studies are needed to determine the effect of testosterone therapy on erectile dysfunction.
Purpose of review
Sclerosing angiomatoid nodular transformation of the spleen (SANT) is a rare benign tumor. Its epidemiological and morphological characteristics remain unknown. The main objective ...of this work is to reveal the epidemiological, biological and morphological characteristics of a SANT.
Recent findings
Seventy-three publications were included in this review describing 188 cases of SANT. The average age of the patients was 45.9 years and the gender ratio was 0.66. SANT was found incidentally in 60% of the cases. In most cases (85.6%), there was no anomaly in biological tests. Abdominal ultrasound showed a hypoechoic mass which is heterogeneous. Abdominal CT scan was performed in 49.5% of the cases. It showed a hypodense (92%), heterogeneous (92%) mass. After contrast agent injection, the spoke wheel aspect was observed (100%). In all cases, the density of the mass was lower than that of the non-tumoral splenic parenchyma. Abdominal MRI was performed in 23.9% of the cases. After dynamic injection of Gadolinium, it showed the same aspect as in the CT scan. All patients were operated either immediately (88.2%) or after clinical and/or radiological changes during monitoring (9.6%).
Summary
The diagnosis of SANT remains a real challenge for the clinician and the radiologist. Establishing the differential diagnosis with other rare tumors is important in order to develop a standardized management protocol.
In the present investigation, extracts obtained from L. guyonianum Durieu ex Boiss. aerial parts were used to evaluate total phenolic, flavonoid and tannin contents. A study of antioxidant activities ...of the prepared samples was carried out on the basis of 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2-2'-azino-bis-(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS+.) and ferric reducing antioxidant power (FRAP) assays. Moreover, the efficiency of methanolic, chloroformic and petroleum ether extracts and the deriving fractions from the methanolic extract was tested against human bacterial and fungal pathogenic strains using micro dilution method in 96 multiwell microtiter plate. Furthermore, leaves and stems extracts were subjected to RP-HPLC for phenolic compounds identification. Results showed that polyphenolic contents and antioxidant activities varied considerably as function of solvent polarity. Moreover, antiradical capacities against DPPH, ABTS(+.) and reducing power were maxima in methanol aerial parts extract which showed the highest polyphenol contents (134mg CE/g DW). The antimicrobial activities showed that methanolic, chloroformic and petroleum ether extracts were found to be most potent against Pseudomonas aeruginosa and Staphylococcus aureus with MIC values of 23 and 46μ.mL(-1), respectively. The fractions F(13) and F(16) have a great antifungal potential against Candida glabrata, Candida krusei and Candida parapsilesis (MIC=39μ.mL(-1)). The RP-HPLC analysis lead the identification of gallic, procatechuic and trans-cinnamic acids, methyl-4-hydroxybenzoate, n-propyl-3,4,5-trihydroxybenzoate, epicatechin, naringin and myricetin in L. guyonianum Durieu ex Boiss. leaves and stems extracts.
Les polyradiculonévrites inflammatoires chroniques (PRNC) sont des neuropathies démyélinisantes chroniques d’origine dysimmunitaire dont le diagnostic repose sur des données cliniques, biologiques et ...électrophysiologiques.
Étudier le profil épidémiologique, clinique, biologique (LCR), électrophysiologique, les modalités thérapeutiques et évolutives des patients suivis pour PRNC. Comparer nos résultats avec ceux de la littérature.
Il s’agit d’une étude rétrospective observationnelle des patients suivis, durant 26ans (de janvier 1988 à décembre 2014), au service de neurologie du CHU de Sfax, Tunisie, pour PRNC. Nous avons recueilli toutes les données cliniques et para-cliniques sur une fiche préétablie. Le diagnostic électrophysiologique de PRNC était révisé selon les nouveaux critères EFNS 2010. Puis nous avons comparé nos résultats avec ceux de la littérature.
Nous avons colligé 18 patients. Le sex-ratio était de 3,5 et l’âge moyen au diagnostic de 48ans. Les troubles de la marche étaient le signe fonctionnel le plus fréquent (95 %). La relecture de l’ENMG a confirmé le diagnostic de PRNC selon les critères EFNS 2010 pour 14 patients. La forme idiopathique était retrouvée dans 50 % des cas. La PRNC était secondaire à une maladie générale dans 45 % des cas. Tous les patients ont été mis sous corticoïdes avec notion de corticorésistance dans 4 cas.
Nous avons noté une nette prédominance masculine, les autres données épidémiologiques étaient concordantes aux données de la littérature. Dans notre série les formes secondaires étaient fréquentes. Le diagnostic précoce permettra d’introduire un traitement immunomodulateur efficace. L’évolution sous corticothérapie était bonne pour la majorité de nos patients (75 %). La rémission complète n’était obtenue que chez un seul patient.
L’utilisation des nouveaux critères EFNS 2010 a permis de d’affiner le diagnostic des PRNC. Le pronostic de ces derniers dépend de l’étiologie en cause.
The title compound, dirubidium dimanganese(II) tris-(tetra-oxo-molyb-date), Rb2Mn2(MoO4)3, was prepared by solid-state reactions. The structure can be described as being composed of MnO6 octa-hedra ...sharing corners with MoO4 tetra-hedra. The three-dimensional framework contains cavities in which the rubidium ions are located. The Rb(+) cations are within distorted nine- and 12-vertex polyhedra. The pairs of different Mn(2+) and Rb(+) cations are each located on threefold rotation axes.. Rb2Mn2(MoO4)3 is isotypic with compounds of the Cs2 M 2Mo3O12 (M = Ni, Fe) family. A comparative structural description is provided between the structure of the title compound and those of related phases. Differences with structures such as alluaudite are discussed.
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