The study of morphological and physiological responses of shrubs to climate is crucial for the understanding of future scenarios regarding climate change. In this light, studying shrub growth and ...physiological acclimation along an elevation gradient might be insightful. The phenolic metabolic pathway represents a powerful tool to interpret such processes.
In the South‐Eastern Alps, we investigated the relationships between elevation, plant traits (i.e. age, xylem ring width, annual shoot length), plant–plant interaction (i.e. shrub cover) and flavonoids in Vaccinium myrtillus L. (leaves, berries) in stands above the treeline. The relationships were parsed within causal networks using a confirmatory path analysis.
Elevation was the main driver of V. myrtillus growth, having both direct and indirect effects on the leaf flavonoid content, but this was less evident for berries. In particular, the content of foliar flavonoids showed a peak at mid‐elevation and where the growth of xylem rings was intermediate, while it decreased in stands with higher shoot length. Flavonoid content variability of both leaves and berries was affected by elevation and shoot length. In berries, flavonoid variability was further related to all growth traits and shrub cover.
These findings evidence that flavonoid content is influenced by both elevation and growth traits of V. myrtillus, often showing non‐linear relationships. These results suggest a trait‐mediated response of this plant to climate conditions as a result of trade‐offs between plant growth, plant defence, environmental stress and nutrient/resource availability.
Elevation is a major driver for the stimulation of plant flavonoid production in response to environmental stress, but the interplay of elevation with plant growth and plant‐plant interactions should never be neglected.
Hemp (Cannabis sativa L.) for industrial use is often considered as a renewable resource for cultivation in polluted areas, on one hand, for the purpose of possible remediation of soil and, on the ...other hand, for wide options of the possible use of plant material after the harvest in such areas, namely hemp fiber. This study summarizes the effects of landfill leachate irrigation on agronomic parameters and fiber properties in two cultivars of hemp has grown and harvested in two consecutive seasons. Cultivar Bialobrzeskie shows more promising results in terms of total fiber content and phloem fiber content, while it tends to sacrifice the density of both xylem and phloem fiber in comparison to cultivar Monoica. The two cultivars also demonstrate different sensitivity and responses to the presence of heavy metals, namely zinc and chromium.
During maturation, Vitis vinifera berries accumulate a large amount of several anthocyanins in the epidermal tissue, whereas their precursors and intermediates are ubiquitously synthesized within the ...fruit. Up to date, several mechanisms of flavonoid transport at subcellular level have been hypothesized, but it is not possible to identify a general model applicable in every plant tissue and organ. Recently, a putative anthocyanin carrier, homologue to mammalian bilitranslocase (BTL) (TC 2.A.65.1.1), was found in Dianthus caryophyllus petal microsomes. In the present paper, an immunohistochemical and immunochemical analysis, using an antibody raised against a BTL epitope, evidences the expression and function of such a transporter in V. vinifera berries (cv. Merlot). Specific localisations of the putative carrier within berry tissues together with expression changes during different developmental stages are shown. Water stress induces an increase in protein expression in both skin and pulp samples. A bromosulfalein (BSP) uptake activity, inhibitable by the BTL antibody, is detected in berry mesocarp microsomes, with K m = 2.39 μM BSP and V max = 0.29 μmol BSP min⁻¹ mg⁻¹ protein. This BSP uptake is also competitively inhibited by quercetin (K i = 4 μM). A putative role for this carrier is discussed in relation to the membrane transport of secondary metabolites.
A homologue of the mammalian bilirubin transporter bilitranslocase (BTL) (TCDB 2.A.65.1.1), able to perform an apparent secondary active transport of flavonoids, has previously been found in ...carnation petals and red grape berries. In the present work, a BTL homologue was also shown in white berries from Vitis vinifera L. cv. Tocai/Friulano, using anti-sequence antibodies specific for rat liver BTL. This transporter, similarly to what found in red grape, was localized in the first layers of the epidermal tissue and in the vascular bundle cells of the mesocarp. In addition, a strong immunochemical reaction was detected in the placental tissue and particularly in peripheral integuments of the seed. The protein was expressed during the last maturation stages in both skin and pulp tissues and exhibited an apparent molecular mass of c. 31 kDa. Furthermore, the transport activity of such a carrier, measured as bromosulphophthalein (BSP) uptake, was detected in berry pulp microsomes, where it was inhibited by specific anti-BTL antibodies. The BTL homologue activity exhibited higher values, for both Km and Vmax, than those found in the red cultivar. Moreover, two non-pigmented flavonoids, such as quercetin (a flavonol) and eriodictyol (a flavanone), inhibited the uptake of BSP in an uncompetitive manner. Such results strengthen the hypothesis that this BTL homologue acts as a carrier involved also in the membrane transport of colourless flavonoids and demonstrate the presence of such a carrier in different organs and tissues.
In this paper, both biochemical and immunochemical evidence for the presence of lipoxygenase (LOX) in plant mitochondria is presented. Highly purified pea (Pisum sativum L., cv. Alaska) mitochondria ...show LOX activity, evaluated as conjugated diene formation, oxygen consumption, and hydroperoxide formation. Both 9- and 13-hydroperoxy-octadecadienoic acids are produced by the oxidation of linoleic acid. LOX activity is particularly evident in swollen mitochondria; it is inhibited by nordihydroguaiaretic acid, a pea anti-LOX B antibody, and has two pH optima (6.0 and 7.5). A mitochondrial protein of ∼97 kDa cross-reacts with a pea seed anti-LOX B antibody. This reaction is detectable in both soluble (matrix fraction) and membrane-bound (submitochondrial particles) proteins. Considering that pea mitochondria were extracted from actively growing stems that were differentiating tube elements, it is suggested that the presence of LOX in these organelles may be related to their degradation linked to xylem differentiation.
Early results from the Solenoidal Tracker At RHIC (STAR) using prototype forward calorimeters (FPD/FPD++) have shown that in d+Au collisions at
= 200 GeV a stronger suppression of forward
π
0
yield ...is observed than would be expected from shadowing effects. Exploratory measurements and observations can be qualitatively interpreted using the Color Glass Condensate (CGC) model to describe the colliding nucleus. A new detector, the Forward Meson Spectrometer (FMS), has been built to measure forward pion and photon production to quantify gluon suppression at low
x
in heavy nuclei over a large range in
x
and
p
T
.
Identified charged-particle spectra of {pi}{sup {+-}}, K{sup {+-}}, p, and p at midrapidity (|y|<0.1) measured by the dE/dx method in the STAR (solenoidal tracker at the BNL Relativistic Heavy Ion ...Collider) time projection chamber are reported for pp and d+Au collisions at {radical}(s{sub NN})=200 GeV and for Au+Au collisions at 62.4, 130, and 200 GeV. Average transverse momenta, total particle production, particle yield ratios, strangeness, and baryon production rates are investigated as a function of the collision system and centrality. The transverse momentum spectra are found to be flatter for heavy particles than for light particles in all collision systems; the effect is more prominent for more central collisions. The extracted average transverse momentum of each particle species follows a trend determined by the total charged-particle multiplicity density. The Bjorken energy density estimate is at least several GeV/fm{sup 3} for a formation time less than 1 fm/c. A significantly larger net-baryon density and a stronger increase of the net-baryon density with centrality are found in Au+Au collisions at 62.4 GeV than at the two higher energies. Antibaryon production relative to total particle multiplicity is found to be constant over centrality, but increases with the collision energy. Strangeness production relative to total particle multiplicity is similar at the three measured RHIC energies. Relative strangeness production increases quickly with centrality in peripheral Au+Au collisions, to a value about 50% above the pp value, and remains rather constant in more central collisions. Bulk freeze-out properties are extracted from thermal equilibrium model and hydrodynamics-motivated blast-wave model fits to the data. Resonance decays are found to have little effect on the extracted kinetic freeze-out parameters because of the transverse momentum range of our measurements. The extracted chemical freeze-out temperature is constant, independent of collision system or centrality; its value is close to the predicted phase-transition temperature, suggesting that chemical freeze-out happens in the vicinity of hadronization and the chemical freeze-out temperature is universal despite the vastly different initial conditions in the collision systems. The extracted kinetic freeze-out temperature, while similar to the chemical freeze-out temperature in pp, d+Au, and peripheral Au+Au collisions, drops significantly with centrality in Au+Au collisions, whereas the extracted transverse radial flow velocity increases rapidly with centrality. There appears to be a prolonged period of particle elastic scatterings from chemical to kinetic freeze-out in central Au+Au collisions. The bulk properties extracted at chemical and kinetic freeze-out are observed to evolve smoothly over the measured energy range, collision systems, and collision centralities.
Bromosulfalein is an organic anion dye used in the study of a variety of membrane carriers expressed in animal tissues and involved in transport of drugs and metabolites. The spectrophotometric assay ...of electrogenic bromosulfalein transport in membrane vesicles, isolated from various mammalian organs or tissues, enables to specifically measure the transport activity of bilitranslocase (TCDB 2.A.65.1.1). The latter is a bilirubin- and flavonoid-specific transporter expressed in rat liver, the organ where its function has been best characterized. The spectrophotometric assay of electrogenic bromosulfalein transport requires minimal volumes of membrane vesicles, is completed within 1 min, and, therefore, is a useful tool to screen the transporter spectrum of potential substrates, by testing them as reversible inhibitors of bromosulfalein transport kinetics. Furthermore, the assay enables to study the progress of time-dependent inactivation of bromosulfalein transport, caused by different protein-specific reagents, including specific anti-sequence antibodies. Inactivation can be retarded by the presence of substrates in a concentration-dependent manner, enabling to derive the dissociation constants of the transporter-substrate complex and thus to gain further insight into the transporter structure-function relationship. This assay, implemented in membrane vesicles isolated from plant organs, has paved the way to the discovery of homologues of bilitranslocase in plants.
PDF
