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► CMCS scaffolds were coated with HAP of different morphology and coverage. ► High HAP coverage on scaffold enhances osteoblast attachment and proliferation. ► HAP coatings enhance ...osteoblastic differentiation of the osteoblasts and stem cells. ► Minimal effect of HAP morphology on enhancement of osteoblastic differentiation.
The behavior of MC3T3 osteoblasts and human bone marrow stem cells on non-coated and hydroxyapatite (HAP)-coated carboxymethyl chitosan (CMCS) scaffolds was investigated in this study. Four HAP-coated scaffolds with different coating morphology and coverage were prepared by mineralization for 1week in four different mineralizing solutions. Viability, attachment, proliferation, and differentiation of the osteoblasts on these scaffolds were evaluated, and an osteogenic gene expression analysis was carried out to investigate the osteoblastic differentiation of the stem cells. No cytotoxic effects were observed with both the non-coated and coated scaffolds. The non-coated CMCS scaffold supports attachment, proliferation, and differentiation of the osteoblasts and directs stem cell differentiation to osteoblast. Coating the scaffold with HAP substantially enhances these effects on the osteoblasts and stem cells. The main improvement was in the late stage of osteoblast differentiation since osteoblastic differentiation of the osteoblasts and stem cells in this stage was significantly enhanced by the coatings regardless of the variation in morphology and coverage. On the other hand, high HAP coverage was beneficial in stimulating osteoblast attachment and proliferation. This study demonstrates the good potential of HAP-coated CMCS scaffolds as osteogenic scaffolds to stimulate bone healing.
This study compares the efficacy of growth factors that are covalently immobilized to those that are adsorbed in improving the bioactivity of a biomaterial. Bone morphogenetic protein-2 (BMP-2) or ...fibroblast growth factor-2 (FGF-2) was covalently bonded to chitosan films using carbodiimide chemistry. For BMP-2, a growth factor loading efficiency of ∼64% was obtained with this method compared to ∼25% from adsorption. As for FGF-2, the growth factor loading efficiency of the two methods was similar at ∼50%. The covalently immobilized BMP-2 promoted attachment, proliferation, and differentiation of osteoblasts in a dose-dependent manner, whereas the covalently immobilized FGF-2 stimulated fibroblast attachment, proliferation, and collagen synthesis. After three weeks immersion in phosphate buffered saline, about 80% of the covalently immobilized growth factors were retained on the films, while only ∼16 and ∼21% of the adsorbed BMP-2 and FGF-2 remained on the corresponding films. The higher retention rate of the covalently immobilized growth factors enabled their stimulatory effects to persist for a longer period than when adsorbed growth factors were used.
Celotno besedilo
Dostopno za:
BFBNIB, DOBA, GIS, IJS, IZUM, KILJ, KISLJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
An alginate-loofa matrix was developed as a cell carrier for ethanol fermentation owing to its porous structure and strong fibrous nature. The matrix was effective for cell immobilization and had ...good mechanical strength and stability for long-term use. After a storage period of 4 months, yeast cells remained firmly immobilized and active.
