This study aims to quantify exhaust/non-exhaust emissions and the uncertainties associated with them by combining innovative motorway tunnel sampling and source apportionment modelling. Analytical ...techniques ICP-AES and GC–MS were used to identify the metallic and organic composition of PM10, respectively. Good correlation was observed between Fe, Cu, Mn, Ni, Pb and Sb and change in traffic volume. The concentration of polycyclic aromatic hydrocarbons and other organics varies significantly at the entrance and exit site of the tunnel, with fluoranthene, pyrene, benzoapyrene, chrysene and benzothiazole having the highest incremented concentrations. The application of Principal Component Analysis and Multiple Linear Regression Analysis helped to identify the emission sources for 82% of the total PM10 mass inside the tunnel. Identified sources include resuspension (27%), diesel exhaust emissions (21%), petrol exhaust emissions (12%), brake wear emissions (11%) and road surface wear (11%). This study shows that major health related chemical species of PM10 originate from non-exhaust sources, further signifying the need for legislation to reduce these emissions.
•Identifies major sources of traffic emissions by using a real world motorway tunnel.•Major non-exhaust sources include resuspension, brake wear and road surface wear.•Several toxic metals (e.g. Fe, Cu, Mn, Ni, Pb, Sb) and PAHs show high incremented levels.•Diesel (21%) & petrol (12%) exhaust emissions also contribute as major sources of PM10.•A number of toxic chemical species of PM10 originate from non-exhaust sources.
•Cereal lipids were analysed using GC/Q-TOF MS.•Differences in sterol, tocopherol and alkylresorcinol patterns could be observed.•Novel tocomonoenols and methyl-alkylresorcinols were detected.•The ...cereals could be discriminated by PCA based on non-targeted lipid profiles.•Non-targeted lipid profiling could be a new tool for cereal authenticity.
Minor lipids in cereals (such as phytosterols and alkylresorcinols) can be important for human nutrition and/or be used as biomarkers for cereal intake. However, the analysis of cereal lipids is very challenging due to the complex lipidome comprising several hundred individual compounds present over a wide range of concentrations.
Here we present a method for the profiling of cereal lipids using high temperature gas chromatography coupled to high resolution mass spectrometry (GC/Q-TOF MS). The method was used to investigate the lipid profiles of 77 samples of bread wheat, spelt, einkorn, emmer, barley, rye and oats.
Distinct differences in the patterns of alkylresorcinols, free and conjugated sterols and tocopherols between the cereals could be observed. Furthermore, traces of tocomonoenols and diunsaturated and methyl-alkylresorcinols (not previously reported in cereals) could be detected. Finally, the lipid patterns in the cereals could be used to separate the cereals by Principal Component Analysis.
Fossil melanin granules (melanosomes) are an important resource for inferring the evolutionary history of colour and its functions in animals. The taphonomy of melanin and melanosomes, however, is ...incompletely understood. In particular, the chemical processes responsible for melanosome preservation have not been investigated. As a result, the origins of sulfur‐bearing compounds in fossil melanosomes are difficult to resolve. This has implications for interpretations of original colour in fossils based on potential sulfur‐rich phaeomelanosomes. Here we use pyrolysis gas chromatography mass spectrometry (Py‐GCMS), fourier transform infrared spectroscopy (FTIR) and time of flight secondary ion mass spectrometry (ToF‐SIMS) to assess the mode of preservation of fossil microstructures, confirmed as melanosomes based on the presence of melanin, preserved in frogs from the Late Miocene Libros biota (NE Spain). Our results reveal a high abundance of organosulfur compounds and non‐sulfurized fatty acid methyl esters in both the fossil tissues and host sediment; chemical signatures in the fossil tissues are inconsistent with preservation of phaeomelanin. Our results reflect preservation via the diagenetic incorporation of sulfur, i.e. sulfurization (natural vulcanization), and other polymerization processes. Organosulfur compounds and/or elevated concentrations of sulfur have been reported from melanosomes preserved in various invertebrate and vertebrate fossils and depositional settings, suggesting that preservation through sulfurization is likely to be widespread. Future studies of sulfur‐rich fossil melanosomes require that the geochemistry of the host sediment is tested for evidence of sulfurization in order to constrain interpretations of potential phaeomelanosomes and thus of original integumentary colour in fossils.
Today, insular Southeast Asia is important for both its remarkably rich biodiversity and globally significant roles in atmospheric and oceanic circulation. Despite the fundamental importance of ...environmental history for diversity and conservation, there is little primary evidence concerning the nature of vegetation in north equatorial Southeast Asia during the Last Glacial Period (LGP). As a result, even the general distribution of vegetation during the Last Glacial Maximum is debated. Here we show, using the stable carbon isotope composition of ancient cave guano profiles, that there was a substantial forest contraction during the LGP on both peninsular Malaysia and Palawan, while rainforest was maintained in northern Borneo. These results directly support rainforest "refugia" hypotheses and provide evidence that environmental barriers likely reduced genetic mixing between Borneo and Sumatra flora and fauna. Moreover, it sheds light on possible early human dispersal events.
Little is known about the types of intestinal parasites that infected people living in prehistoric Britain. The Late Bronze Age archaeological site of Must Farm was a pile-dwelling settlement located ...in a wetland, consisting of stilted timber structures constructed over a slow-moving freshwater channel. At excavation, sediment samples were collected from occupation deposits around the timber structures. Fifteen coprolites were also hand-recovered from the occupation deposits; four were identified as human and seven as canine, using fecal lipid biomarkers. Digital light microscopy was used to identify preserved helminth eggs in the sediment and coprolites. Eggs of fish tapeworm (Diphyllobothrium latum and Diphyllobothrium dendriticum), Echinostoma sp., giant kidney worm (Dioctophyma renale), probable pig whipworm (Trichuris suis) and Capillaria sp. were found. This is the earliest evidence for fish tapeworm, Echinostoma worm, Capillaria worm and the giant kidney worm so far identified in Britain. It appears that the wetland environment of the settlement contributed to establishing parasite diversity and put the inhabitants at risk of infection by helminth species spread by eating raw fish, frogs or molluscs that flourish in freshwater aquatic environments, conversely the wetland may also have protected them from infection by certain geohelminths.
Seventy-one individuals from the late Neolithic population of the 7000-year-old site of Hódmezővásárhely-Gorzsa were examined for their skeletal palaeopathology. This revealed numerous cases of ...infections and non-specific stress indicators in juveniles and adults, metabolic diseases in juveniles, and evidence of trauma and mechanical changes in adults. Several cases showed potential signs of tuberculosis, particularly the remains of the individual HGO-53. This is an important finding that has significant implications for our understanding of this community. The aim of the present study was to seek biomolecular evidence to confirm this diagnosis. HGO-53 was a young male with a striking case of hypertrophic pulmonary osteopathy (HPO), revealing rib changes and cavitations in the vertebral bodies. The initial macroscopic diagnosis of HPO secondary to tuberculosis was confirmed by analysis of Mycobacterium tuberculosis complex specific cell wall lipid biomarkers and corroborated by ancient DNA (aDNA) analysis. This case is the earliest known classical case of HPO on an adult human skeleton and is one of the oldest palaeopathological and palaeomicrobiological tuberculosis cases to date.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Faced with terrestrial threats, land plants seal their aerial surfaces with a lipid-rich cuticle. To breathe, plants interrupt their cuticles with adjustable epidermal pores, called stomata, that ...regulate gas exchange, and develop other specialised epidermal cells such as defensive hairs. Mechanisms coordinating epidermal features remain poorly understood. Addressing this, we studied two loci whose allelic variation causes both cuticular wax-deficiency and misarranged stomata in barley, identifying the underlying genes, Cer-g/ HvYDA1, encoding a YODA-like (YDA) MAPKKK, and Cer-s/ HvBRX-Solo, encoding a single BREVIS-RADIX (BRX) domain protein. Both genes control cuticular integrity, the spacing and identity of epidermal cells, and barley's distinctive epicuticular wax blooms, as well as stomatal patterning in elevated CO
conditions. Genetic analyses revealed epistatic and modifying relationships between HvYDA1 and HvBRX-Solo, intimating that their products participate in interacting pathway(s) linking epidermal patterning with cuticular properties in barley. This may represent a mechanism for coordinating multiple adaptive features of the land plant epidermis in a cultivated cereal.
Both leaves and petals are covered in a cuticle, which itself contains and is covered by cuticular waxes. The waxes perform various roles in plants' lives, and the cuticular composition of leaves has ...received much attention. To date, the cuticular composition of petals has been largely ignored. Being the outermost boundary between the plant and the environment, the cuticle is the first point of contact between a flower and a pollinator, yet we know little about how plant-pollinator interactions shape its chemical composition. Here, we investigate the general structure and composition of floral cuticular waxes by analysing the cuticular composition of leaves and petals of 49 plant species, representing 19 orders and 27 families. We show that the flowers of plants from across the phylogenetic range are nearly devoid of wax crystals and that the total wax load of leaves in 90% of the species is higher than that of petals. The proportion of alkanes is higher, and the chain lengths of the aliphatic compounds are shorter in petals than in leaves. We argue these differences are a result of adaptation to the different roles leaves and petals play in plant biology.
Magmatic intrusion and faulting both accommodate crustal extension in magma‐rich rifts. However, quantitative constraints on the contribution of faulting to total extension and along‐rift variations ...of faulting during the final stages of break‐up are lacking. We targeted the Danakil Depression (Afar, Ethiopia) to conduct a quantitative, high‐resolution study of fault activity and interaction in a magma‐rich rift near break‐up. Quantitative analysis of >500 rift axis faults, identified using remote sensing data (satellite imagery, DEMs), shows an increase in fault density, length and connectivity away from magmatic segments. Kinematic and earthquake focal mechanism data demonstrate a transition from transtensional opening in the northern and central sub‐regions of the rift to oblique opening in the southern Giulietti Plain and Tat‐Ali sub‐regions. Oblique opening is attributed to the along‐axis step between the Erta‐Ale and Harak sub‐regions. Integration of seismic reflection and borehole data with the mapped faults shows that extension is primarily accommodated by magmatism within the rift center, with faulting more significant toward the ends of the rift. ∼30% of crustal extension is accommodated by axial faulting in areas of low magmatism, highlighting the importance of faulting even in the final stages of magma‐rich rifting. Comparing our findings with spreading ridge morphology and structure, relevant due to the rift maturity and extensive magmatism, we conclude that the Danakil Depression is in a transitional stage between continental rifting and seafloor spreading. Spatial changes in the importance of faulting and magmatism in accommodating extension, alongside rift morphology, resemble the relationships observed along spreading ridges.
Key Points
Switch from transtensional to oblique opening along the rift correlates to lateral steps of rift segments
At least 30% of extension in magma‐poor areas of the depression is accommodated by upper crustal axial faulting
Characteristics and spatial distribution of axial faulting in the Danakil Depression mimics that observed along seafloor spreading ridges
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