Rapid, sensitive and inexpensive analysis of biological molecules is vital to many fundamental problems in molecular biology. Nanomechanical resonators are of great interest for such applications. ...The use of these devices for the analysis of protein mixtures would however require the immobilization of probes onto their surfaces in order to enable the specificity of the detection. Such nanoresonator-based specific detection of proteins is here reported using streptavidin as target system, and immobilized biotin as probe. Nanomechanical resonators resistant to stiction were first realized from silicon carbonitride using a novel fabrication method. Vapor-phase deposition of mercaptopropyl trimethoxysilane was performed, and an added mass of 2.22
±
0.07
fg/μm
2 was measured. This linker molecule was used to attach biotin onto the devices, enabling the specific detection of streptavidin. A mass of 3.6
fg/μm
2 was attributed to the added streptavidin, corresponding to one molecule per 27
nm
2. The specificity of this recognition was confirmed by exposing the devices to a solution of streptavidin that was already saturated with biotin. An additional negative control was also performed by also exposing bare devices to streptavidin in absence of the attached biotin. No resonance frequency shift was observed in either case, confirming the specificity of the detection.
The fast method of the simultaneous determination of F−, Cl−, Br− and SO42− anions in the deionized water on the trace level by ion chromatography using thorough cleaning of respective water ...containers, 10μM NaHCO3 water solution as eluent, short Metrohm (50×4mm) separation column and a large water volume injection is proposed. Calculated detection limits are 10−9–10−8% depending on the element.
The method for the fast screening of plant oil samples for the total fluorine-, chlorine-, bromine- and sulfur-organic compounds content (calculated for the respective elements) on the trace level is developed.
It is based on the high temperature combustion of oil sample in oxygen flow, absoption of the conversion products in deionized water and whole absorbate volume analysis for F−, Cl−, Br− and SO42− anions, corresponding to the respective elements, using the developed method of these anions analysis by ion chromatography.
The samples of soya, olive, sunflower and cotton seed oil were analyzed. The method detection limits (for 1mg sample) were 2×10−6%, 2×10−6%, 5×10−6% and 5×10−6% for fluorine, chlorine, bromine and sulfur, respectively. The relative standard deviation was≤15%. The method gives the compressed information about the total content of all target and nontarget fluorine-, chlorine-, bromine- and sulfur-organic compounds in plant oils.
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•High sensitive determination of the anions in the deionised water.•High sensitive method of plant oil screening for halogen and S-organic compounds.•Method detection limits were 20–50 p.p.b. (sample – 1 mg).•Fast screening of plant oil samples for halogen and S-organic compounds.
BANK 3.0 CONCEPT: GLOBAL TRENDS AND IMPLICATIONS Vasylieva, T. A.; Leonov, S. V.; Kryvych, Ya. N. ...
Fìnansovo-kreditna dìâlʹnìstʹ: problemi teorìì̈ ta praktiki (Online),
06/2017, Letnik:
1, Številka:
22
Journal Article
Recenzirano
Odprti dostop
Objective is to make the review of the banking industry development to identify theoretical and practical areas of transformations under the concept Bank 3.0.Results. Theoretical and practical shifts ...occured in modern banking sector in the conditions of Bank 3.0 concept implementation are identified. Background of the modern transformation in the banking industry on a global scale is identified, the role and extent of structural changes in the banking sector is assessed and features of the modern business models of banks are identified.Originality. Theoretical framework of banking transformations as financial intermediaries is developed. Distinguished features of the bank business–model in the future including high technology, giditalization of commercial activity, transformation of banking infrastructure, banking sector integration with social networks, instant messengers and Internet platforms, are justified.Practical relevance. Identified features of the bank business–model can be used in the activities of banking developing the strategy and tactics to distribute banking services.
Microphotoluminescence (μPL) spectra of single In(Ga)P/GaInP quantum dots (QD) were investigated. Measurements were carried out at different optical pump power and at different electric field with ...constant optical pump. Filling of electron s-, p-, d-states was observed. Quantum confinement Stark effect (QCSE) was detected. Obtained PL life times of s-, p- electron states were 0.5 ns and 0.4 ns respectively. From spectra it is clearly seen that QDs have weak quantum confinement (hω0∼4-8meV).
We confront the terrible reality that systemic racism and discrimination impacts the daily personal and professional lives of many members of the scientific community and broader society. In the ...U.S., the brutal killing of George Floyd while in police custody is one of the most recent examples of the centuries of systemic violence suffered by Black Americans. This moment and its aftermath lay bare the legacies of racism and its exclusionary practices. Let us be clear: we, the Editors, Staff, and Governance Members of ACS Publications condemn the tragic deaths of Black people and stand in solidarity with Black members of the science and engineering community. Moreover, ACS condemns racism, discrimination, and harassment in all forms. We will not tolerate practices and viewpoints that exclude or demean any member of our community. Despite these good intentions, we recognize that our community has not done enough to provide an environment for Black chemists to thrive. Rep. Eddie Bernice Johnson, Chairwoman of the U.S. House Committee on Science, Space, and Technology said, "So far, we have gotten by with a STEM workforce that does not come close to representing the diversity of our nation. However, if we continue to leave behind so much of our nation's brainpower, we cannot succeed." 1 Indeed, the U.S. National Science Foundation notes that Blacks and other under-represented minority groups continue to be under-represented in science and engineering education and employment. 2 What is abundantly clear in this moment is that this lack of representation is a symptom of systemic racism across all levels of education and professional life. We know that supportive words are not enough. We must develop and implement a concrete plan for changing our trajectory. Publications and citations are academic currency, and while we like to think publishing a manuscript is "just about the science", we know that is not true for everyone. We have seen the biases (largely through the lens of gender and in Western countries because of the limitations in bibliometric analyses) and applaud our colleagues at the RSC for their massive study that explored these gender barriers in the publishing pipeline 3 and their recent Inclusion and Diversity Framework. 4 At the present time, unfortunately, less is known about the effects of race and ethnicity on publishing success. A study published in PeerJ, however, found that unprofessional reviewer comments had a disproportionate effect on authors from under-represented groups. 5 As the world's leading society publisher, we have a responsibility to aggressively combat bias in all aspects of the publishing process, including systemic under-representation of Blacks in this endeavor (no ACS journal is currently led by a Black Editor-in-Chief). Within ACS Publications, we actively track gender and geographic diversity of editors, advisors, authors, and reviewers, and we anecdotally report on race of editors. Diversity encompasses many more dimensions than these, and we acknowledge that we can do much more than we have. We affirm that diversity and inclusion strengthen the research community and its impact, and we are committed to developing, implementing, tracking, and reporting on our progress to ensure that our editors, advisors, reviewers, and authors are more diverse and that all authors receive the same fair treatment and opportunity to publish in our journals. We acknowledge that we do not have all the answers now, but we seek to hear from and listen to our community on how we can improve our journals to be more diverse and inclusive. As first steps, we commit to the taking the following actions: • Gathering and making public our baseline statistics on diversity within our journals, encompassing our editors, advisors, reviewers, and authors; annually reporting on progress • Training new and existing editors to recognize and interrupt bias in peer review • Including diversity of journal contributors as an explicit measurement of Editor-in-Chief performance • Appointing an ombudsperson to serve as a liaison between Editors and our Community • Developing an actionable diversity plan for each ACS journal These are only initial plans and the start of a conversation: other ideas are beginning to germinate, and we commit to sharing them with you regularly. We invite you contribute your ideas on how we can do better via our Axial website. We are listening carefully. We encourage you to take immediate action in your own circles. In a recent editorial, JACS Associate Editor Melanie Sanford 6 offered practical steps to take now. Take a moment to find out more about these actions and how to bring them into your work and your life. We all have a responsibility to eradicate racism and discrimination in the science and engineering community; indeed, to make a real difference, we need to be antiracist. The tragic events we have seen in the Black community provide great urgency to this goal. The work will be difficult and will force us to confront hard realities about our beliefs and actions. We fully expect that you, and everyone in the community, will hold us accountable.
Мета роботи – обговорення можливості застосування ситуаційних завдань для формування клінічного мислення у студентів.
Основна частина. Одним із головних завдань навчання студентів-медиків є ...формування клінічного мислення, що дозволяє надалі самостійно працювати з хворими як під час навчання на старших курсах, так і в інтернатурі. Беручи до уваги, що проведення практичних занять передбачає тестовий контроль знань на кожному занятті, з одного боку, а з іншого – передбачає відпрацювання практичних навичок з обстеження хворого, це певним чином обмежує можливості розвитку у студентів клінічного мислення.
Поточний контроль – це не тільки перевірка рівня засвоєння матеріалу заняття, це ще й продовження навчання, повторення основних питань теми, систематизація знань та вмінь, а також їх закріплення.
Контроль за допомогою ситуаційних завдань покликаний підбити підсумок освоєння кожного розділу, підсумовувати знання студента, дати йому можливість систематизувати свої уявлення про провідні синдроми і клінічні ознаки захворювань різних систем та органів, дозволяє внести корективи в його розуміння законів функціонування організму в цілому.
Таким чином, частка клінічного мислення в рішенні ситуаційних задач дуже значна, і значення їх не вичерпується тільки контролем поточної підготовки по тій чи іншій темі, а є проміжним тренувальним заходом у системі підготовки.
Висновок. Таким чином, клінічні ситуаційні завдання можна використовувати не тільки для контролю знань, а й для формування клінічного мислення.
The article is devoted to the problem of clinical manifestation of irritated bowels syndrome among children. It is stated that the leading symptom of suffering - abdominal pian - is combined with the ...psychopathologic symptomatology such as various neurosis-like disorders, which should be taken into consideration while developing therapeutic measures among such patients.
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