Abstract
CUORE (
Cryogenic Underground Observatory for Rare Events
) is a tonne-scale experiment located at the LNGS with the main scientific goal of searching for neutrinoless double beta decay in
...130
Te. The detector consists of TeO
2
crystals operated as cryogenic calorimeters. The use of natural tellurium allows us to search for other isotopes rare decays. The neutrinoless positron emitting electron capture (0
υECβ
+
) of
120
Te (natural abundance 0.09(1)%) has a clear signature from the 511-keV annihilation γ rays. We present an analysis of this process based on a new algorithm to perform the simultaneous spectral fit over five selected decay scenarios. Each scenario is characterized by a set of crystals simultaneously interested by a detectable energy release. We describe the background structure modeling and tests of linearity we performed on the fit algorithm. We finally present the limit setting sensitivity and the next steps of this study, including systematics accounting.
Neutrinoless double beta decay (0νββ) is a rare, second-order nuclear transition that occurs only if neutrinos are massive Majorana particles or through new physics beyond Standard Model. This ...process explicitly violates the lepton number (L) by two units and, therefore, the observation of 0νββ would demonstrate that L is not a symmetry of nature. Combined with flavour mixing and cosmological measurements, it can provide unique contraints on neutrino mass scale and establish whether neutrinos are Dirac or Majorana particles. The Cryogenic Underground Observatory for Rare Events (CUORE) is an experiment located at the LNGS searching for 0νββ decay of 130Te. CUORE exploits the bolometric technique to reach high resolution around the Q-value (2527.5 keV). It consists of an array of 988 natural TeO2 cubic crystals grouped into 19 towers. With a total active mass of 742 kg (~206 kg of 130Te), CUORE is operated at very low temperature with a new 3He/4He refrigerator. Data taking started at the beginning of 2017. After a brief introduction on the detector and the way data analysis is performed, I describe CUORE first results for the search of the 0νββ decay that were published in March 2018.
The c-Jun N-terminal kinase (JNK) has been shown to mediate tamoxifen-induced apoptosis in breast cancer cells. However, the downstream mediators of the JNK pathway linking tamoxifen to effectors of ...apoptosis have yet to be identified. In this study, we analysed whether c-Jun, the major nuclear target of JNK, has a role in tamoxifen-induced apoptosis of SkBr3 breast cancer cells. We show that before DNA fragmentation and caspase 3/7 activation, cytotoxic concentrations of 4-hydroxytamoxifen (OHT) induced JNK-dependent phosphorylation of c-Jun at JNK sites earlier shown to regulate c-Jun-mediated apoptosis. In addition, OHT induced ERK-dependent expression of c-Fos and transactivation of an AP-1-responsive promoter. In particular, the ectopic expression of dominant-negative constructs blocking either AP-1 activity or c-Jun N-terminal phosphorylation prevented DNA fragmentation after OHT treatment. Furthermore, both c-Fos expression and c-Jun N-terminal phosphorylation preceded OHT-dependent activation of caspase 3-7 in different types of tamoxifen-sensitive cancer cells, but not in OHT-resistant LNCaP prostate cancer cells. Taken together, our results indicate that the c-Jun/c-Fos AP-1 complex has a pro-apoptotic role in OHT-treated cancer cells and suggest that pharmacological boosts of c-Jun activation may be useful in a combination therapy setting to sensitize cancer cells to tamoxifen-mediated cell death.
Silicon photomultipliers (SiPM) have gained popularity in particle physics due to their inherent advantages in terms of compactness, low power consumption, and high photon detection efficiency. ...Moreover, we have to deal with signals ranging from a few photoelectrons (PE), where we need to reconstruct the exact shape, up to thousands of PEs in short time intervals.
The design of this amplifier was conceived to handle the SiPMs signal in both cases, combining together a high-speed amplifier for precisely reconstruct the shape of the signals of a few PEs and an integrated one in which the output voltage level is directly proportional to the number of the input PEs.
The single-chain ribosome-inactivating proteins (sc-RIPs) from plant origin are antiviral and antiproliferative agents employed in the preparation of immunotoxins. Similarly to the A-chains of ricin, ...sc-RIPs act as rRNA
N-glycosidases. We demonstrate here that dianthin 30, saporin 6 and gelonin exert a specific nuclease activity on supercoiled DNA. Four specific sites of cleavage introduced by dianthin 30 and by saporin 6 and two specific sites of cleavage introduced by gelonin have been identified and mapped in pBR322.
• Isolation and biochemical characterization is reported here of 5-enol-pyruvyl-shikimate-3-phosphate (EPSP) synthase, the enzyme that catalyses the sixth step in the common prechorismate pathway of ...aromatic amino acid biosynthesis and the target of the widely used herbicide glyphosate, from the cyanobacterium Spirulina platensis. • Homogeneous enzyme preparations were obtained by ammonium sulphate fractionation, anion-exchange and substrate-elution chromatography, and chromatofocusing. Protein characterization was carried out by conventional kinetic analysis, PAGE and gel permeation. • A 2800-fold purification was achieved, with a recovery of 20% of initial activity. Unusually low apparent affinities for both substrates, phosphoenolpyruvate and shikimate-3-phosphate, did not correspond to decreased glyphosate sensitivity. During SDS-PAGE, the protein migrated as a single band corresponding to a molecular mass of c. 49 kDa. The behaviour of the protein upon gel permeation chromatography under nondenaturing conditions was, however, consistent with a mass of c. 91 kDa. • The native enzyme appears to be homodimeric, a remarkable feature that has not been previously reported for EPSP synthases from either cyanobacteria or higher plants. The presence of mono- and dimeric EPSP synthases could represent an important tool for cyanobacterial classification.
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