Development of resistance to sarcoptic mange in ibex Pérez, Jesús M.; López‐Montoya, Antonio J.; Cano‐Manuel, Francisco J. ...
The Journal of wildlife management,
July 2022, 2022-07-00, 20220701, Letnik:
86, Številka:
5
Journal Article
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Sarcoptic mange affects mammal host species worldwide and, particularly, wild Caprinae throughout much of Eurasia. In the Iberian Peninsula, several outbreaks of sarcoptic mange in Iberian ibex ...(Capra pyrenaica) have been reported since the 1980s. Using data from a period of long‐term monitoring and a seasonal autoregressive integrated moving average (SARIMA)‐generalized autoregressive conditional heteroscedasticity (GARCH) model approach, we performed a time‐series analysis of the monthly prevalence of sarcoptic mange in the Iberian ibex population in Sierra Nevada Natural Space in southern Spain. In January 2003–March 2021, we documented a significant negative trend in sarcoptic mange prevalence, albeit with some interannual peaks. These findings can only be explained if a certain level of resistance to sarcoptic mange exists in hosts that, along with other factors, could provoke this reduced prevalence. Prevalence values varied seasonally, with maximum values in spring and minimum values at the end of summer, which may be due to factors linked to climate, host behavior, and endocrine activity. Our model predicts that the prevalence of sarcoptic mange in the Iberian ibex will continue to decrease over the next 2 years. Despite the inherent challenges involved, diagnosing and monitoring of wildlife diseases are integral to obtaining reliable epidemiological data and designing appropriate management strategies.
In 2003–2021 an anlysis of the prevalence of sarcoptic mange in an Ibex population showed a significant decreasing and seasonal trend, which suggests that a degree of resistance to this parasitosis has developed in this host. We also predict a decrease in prevalence values over the following 2 years based on our models. Obtaining reliable data on wildlife disease prevalence and other epidemiological variables is pivotal for the appropriate management of such diseases and long‐term monitoring of these variables can help improve knowledge of how host‐parasite relationships influence the dynamics of diseases.
The microRNAs (miRNAs) are a class of small, 20-22 nucleotides in length, endogenously expressed noncoding RNAs that regulate multiple targets posttranscriptionally. Interestingly, miRNAs have ...emerged as regulators of most physiological and pathological processes, including metastatic tumor progression, in part by controlling a reversible process called epithelial-to-mesenchymal transition (EMT). The activation of EMT increases the migratory and invasive properties fundamental for tumor cell spread while activation of the reverse mesenchymal-to-epithelial transition is required for metastasis outgrowth. The EMT triggering leads to the activation of a core of transcription factors (EMT-TFs) - SNAIL1/SNAIL2, bHLH (E47, E2-2, and TWIST1/TWIST2), and ZEB1/ZEB2 - that act as E-cadherin repressors and, ultimately, coordinate EMT. Recent evidence indicates that several miRNAs regulate the expression of EMT-TFs or EMT-activating signaling pathways. Interestingly, some miRNAs and EMT-TFs form tightly interconnected negative feedback loops that control epithelial cell plasticity, providing self-reinforcing signals and robustness to maintain the epithelial or mesenchymal cell status. Among the most significant feedback loops, we focus on the ZEB/miR-200 and the SNAIL1/miR-34 networks that hold a clear impact in the regulation of the epithelial-mesenchymal state. Recent insights into the p53 modulation of the EMT-TF/miRNA loops and epigenetic regulatory mechanisms in the context of metastasis dissemination will also be discussed. Understanding the regulation of EMT by miRNAs opens new avenues for the diagnosis and prognosis of tumors and identifies potential therapeutic targets that might help to negatively impact on metastasis dissemination and increasing patient survival.
Cell plasticity is emerging as a key regulator of tumor progression and metastasis. During carcinoma dissemination epithelial cells undergo epithelial to mesenchymal transition (EMT) processes ...characterized by the acquisition of migratory/invasive properties, while the reverse, mesenchymal to epithelial transition (MET) process, is also essential for metastasis outgrowth. Different transcription factors, called EMT‐TFs, including Snail, bHLH and Zeb families are drivers of the EMT branch of epithelial plasticity, and can be post‐transcriptionally downregulated by several miRNAs, as the miR‐200 family. The specific or redundant role of different EMT‐TFs and their functional interrelations are not fully understood. To study the interplay between different EMT‐TFs, comprehensive gain and loss‐of‐function studies of Snail1, Snail2 and/or Zeb1 factors were performed in the prototypical MDCK cell model system. We here describe that Snail1 and Zeb1 are mutually required for EMT induction while continuous Snail1 and Snail2 expression, but not Zeb1, is needed for maintenance of the mesenchymal phenotype in MDCK cells. In this model system, EMT is coordinated by Snail1 and Zeb1 through transcriptional and epigenetic downregulation of the miR‐200 family. Interestingly, Snail1 is involved in epigenetic CpG DNA methylation of the miR‐200 loci, essential to maintain the mesenchymal phenotype. The present results thus define a novel functional interplay between Snail and Zeb EMT‐TFs in miR‐200 family regulation providing a molecular link to their previous involvement in the generation of EMT process in vivo.
What's new?
Epithelial‐mesenchymal transition (EMT) is involved in the initiation of metastasis and is regulated by transcription factors (EMT‐TFs) that are themselves regulated by miRNAs. The roles of the different EMT‐TFs and their functional relationships, however, are poorly understood. Here, the EMT‐TFs Snail1 and Zeb1 were found to play an essential role in EMT induction, while mesenchymal phenotype was maintained by continuous Snail1 and Snail2 expression. Snail1 controlled and maintained the mesenchymal phenotype through CpG DNA hypermethylation of miR‐200 loci. Hypermethylated miR‐200 family members may serve as surrogate markers in the treatment or prognosis of carcinosarcomas.
Recently, technological development has become increasingly pronounced, with great advances in all production areas, including agriculture. In the agricultural sector, improvements in machinery and ...the study of new techniques have resulted in the increased efficiency and productivity of different crops. In particular, UAVs (unmanned aerial vehicles) are increasingly used in agriculture. Among the latest utilities is the application of phytosanitary products. The use of small UAVs retrofitted with spraying systems make it possible to treat large surfaces in short periods of time, achieving a reduction in inputs and time compared to conventionally used methods (trailed sprayers). The aim of this work was to compare conventional sprayers and UAV sprayers on a farm in Spain, with olive and vineyard crops. The determining factors in the choice of one or another alternative were studied, such as the economic requirements and efficiency of different alternatives. The results show that a large investment is required to acquire a UAV sprayer, while operating costs are higher for conventional sprayers versus UAV sprayers. Efficiency (ha/h) is much higher for UAV sprayers. In addition to these advantages, UAV sprayers require a lower cost of water and products. However, different factors could make conventional sprayers a better choice.
Yeasts are a diverse group of fungal microorganisms that are widely used to produce fermented foods and beverages. In Mexico, open fermentations are used to obtain spirits from agave plants. Despite ...the prevalence of this traditional practice throughout the country, yeasts have only been isolated and studied from a limited number of distilleries. To systematically describe the diversity of yeast species from open agave fermentations, here we generate the YMX-1.0 culture collection by isolating 4524 strains from 68 sites with diverse climatic, geographical, and biological contexts. We used MALDI-TOF mass spectrometry for taxonomic classification and validated a subset of the strains by ITS and D1/D2 sequencing, which also revealed two potential novel species of Saccharomycetales. Overall, the composition of yeast communities was weakly associated with local variables and types of climate, yet a core set of six species was consistently isolated from most producing regions. To explore the intraspecific variation of the yeasts from agave fermentations, we sequenced the genomes of four isolates of the nonconventional yeast Kazachstania humilis. The genomes of these four strains were substantially distinct from a European isolate of the same species, suggesting that they may belong to different populations. Our work contributes to the understanding and conservation of an open fermentation system of great cultural and economic importance, providing a valuable resource to study the biology and genetic diversity of microorganisms living at the interface of natural and human-associated environments.
Nontypable Haemophilus influenzae (NTHi) is a Gram-negative, non-capsulated human bacterial pathogen, a major cause of a repertoire of respiratory infections, and intimately associated with ...persistent lung bacterial colonization in patients suffering from chronic obstructive pulmonary disease (COPD). Despite its medical relevance, relatively little is known about its mechanisms of pathogenicity. In this study, we found that NTHi invades the airway epithelium by a distinct mechanism, requiring microtubule assembly, lipid rafts integrity, and activation of phosphatidylinositol 3-kinase (PI3K) signalling. We found that the majority of intracellular bacteria are located inside an acidic subcellular compartment, in a metabolically active and non-proliferative state. This NTHi-containing vacuole (NTHi-CV) is endowed with late endosome features, co-localizing with LysoTracker, lamp-1, lamp-2, CD63 and Rab7. The NTHi-CV does not acquire Golgi- or autophagy-related markers. These observations were extended to immortalized and primary human airway epithelial cells. By using NTHi clinical isolates expressing different amounts of phosphocholine (PCho), a major modification of NTHi lipooligosaccharide, on their surfaces, and an isogenic lic1BC mutant strain lacking PCho, we showed that PCho is not responsible for NTHi intracellular location. In sum, this study indicates that NTHi can survive inside airway epithelial cells.
Splenic biopsy is a necessary diagnostic procedure. It has been proved that it is a safe procedure with a high diagnostic accuracy. However, the decision of percutaneous or endoscopic ...ultrasound-guided approach should be assessed taking into consideration patients' characteristics and the experience of each center.
The aim of this study was to identify an easily reliable prognostic score that selects the subset of advanced soft tissue sarcoma (ASTS) patients with a higher benefit with trabectedin in terms of ...time to progression and overall survival. A retrospective series of 357 patients with ASTS treated with trabectedin as second- or further-line in 19 centers across Spain was analyzed. First, it was confirmed that patients with high growth modulation index (GMI > 1.33) were associated with the better clinical outcome. Univariate and multivariate analyses were performed to identify factors associated with a GMI > 1.33. Thus, GEISTRA score was based on metastasis free-interval (MFI ≤ 9.7 months), Karnofsky < 80%, Non L-sarcomas and better response in the previous systemic line. The median GMI was 0.82 (0-69), with 198 patients (55%) with a GMI < 1, 41 (11.5%) with a GMI 1-1.33 and 118 (33.1%) with a GMI > 1.33. The lowest GEISTRA score showed a median of time-to-progression (TTP) and overall survival (OS) of 5.7 and 19.5 months, respectively, whereas it was 1.8 and 3.1 months for TTP and OS, respectively, for the GEISTRA 4 score. This prognostic tool can contribute to better selecting candidates for trabectedin treatment in ASTS.
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