Genetic variation in three Croatian donkey populations, Istrian (IS), North Adriatic (NA) and Littoral‐Dinaric (LD), was analysed using eight microsatellite loci and by sequence and SSCP analysis of ...the proximal portion of the mtDNA D‐loop region. The analysis of microsatellite loci revealed observed heterozygosities in the range of 0.37 (MPZ002 in LD) to 0.85 (AHT21 in LD) and polymorphic information content values in the range of 0.36 (MPZ002 in NA) to 0.78 (AHT21 in LD). The overall probability of exclusion was 0.991. Two populations (IS and NA) were closely related (Fst=0.0034), whereas genetic distances between IS and LD (Fst=0.021) and NA and LD (Fst=0.027) were higher. Using AMOVA, 97.6% of the total genetic variance was portioned within populations, while 2.7% was portioned between the Littoral‐Dinaric population and the Istrian/North Adriatic population group. Sequencing of the proximal part of the mtDNA D‐loop region revealed 36 polymorphic sites representing 19 haplotypes which clustered into three haplotype groups (Y, W, Ws). Only the Y haplotype was found in the IS population which is characterized by a large body size. Haplotypes W and Ws were found in the NA and LD populations which include smaller animals. All three haplotypes were found in the LD population, indicating sporadic migration events from the IS into LD donkey population.
Genetic variation in the Pag island sheep population was analysed using seven microsatellite loci and by sequencing of the proximal part (nt 15816–16153) of the D-loop region at mtDNA. The mean ...number of alleles per microsatellite locus (8.71) and mean expected heterozygosity (0.833) reveal relative high genetic variability. Sequencing of the part of the mtDNA D-loop region from 10 unrelated animals revealed 39 polymorphic sites characterizing seven haplotypes which clustered into two main haplogroups (M and P). Sequence differences among haplotype groups M and P were from 6.78 to 9.44%. Our data support the hypothesis that the M haplotype represents a trace of Merinisation of the Pag island sheep, reflected by the high degree of sequence identity with the Merino breed sequence (99%). The P haplotype belongs to the original primitive sheep that have inhabited the island of Pag for thousands of years. These findings suggest that modern Pag island sheep were derived from at least two maternal lineages, the autochthonous and imported one.
The aim of this research was to identify factors of morphological and basic motor status in young judoka in prediction of fighting efficiency. The subject sample included 57 judoka aged 13 to 15, who ...have been involved in training process for averagely three years. The sample of predictor variables included a set of 18 variables for assessing anthropometric characteristics and a set of 12 variables for assessing basic motor abilities. Factor analysis was used to analyze the structure of morphological characteristics and basic motor abilities, and within the analysis, varimax rotation of principal components of the intercorrelation matrix was conducted. Morphological status of judoka was defined by four factors: longitudinal dimensionality of the skeleton, subcutaneous fat tissue, transverse dimensionality of the skeleton and body mass and volume. Motor status was defined by the following factors: power and coordination (force regulator) factor, movement frequency (speed regulator) factor, flexibility and balance factor and precision factor. Significant impact of morphological-motor factors on situational efficiency in judoka, i.e. success in competitions (multiple correlation was 0.86), was obtained by regression analysis. The best predictor of competition efficiency in young judoka was the factor which integrates explosive power, coordination and muscle endurance, and which is underlain with a force regulation mechanism. The second most important predictor determining fighting efficiency is the factor of movement frequency which is controlled by a speed regulator mechanism.
In 75 donkeys of Croatia that were classified into three types (Littoral-Dinaric, Northern-Adriatic and Istrian) transferrin and albumin polymorphisms were studied by electrophoresis. Transferrin ...allele frequencies indicate the phylogenetic relationship of the Istrian and Northern-Adriatic type of donkey, as well as the significant difference of Littoral-Dinaric type in relation to populations living in the area of Kvarner islands and Istria. Albumin allele frequencies have not indicated a significant difference between the types. Standard genetic distance is the greatest between Littoral-Dinaric and Istrian types and the smallest between Northern-Adriatic and Istrian types of donkey, which also corresponds to the geographical distance of the areas in which these populations live. In order to get more information, it is necessary to direct further research towards determination of frequencies of other polymorphic proteins and direct DNA typing.
Under the assumption that some of the unrecognized Lipizzan lines originate from one of the classical female lines, the main goal of this studies was to prove that stud book data was not kept ...adequately. Since in the framework of LIF the conducted INCO Copernicus project "Biotechnical methods in the maintenance of genetic diversity of the Lipizzan horse breed" comprised the research of the genetic structure of the entire studfarm Lipizzan horse population in European national stud farms, it was possible to compare its results to the results of this research and confirm or disprove set hypothesis. Analysed mares in the stud books of the Stud Farm of Dakovo are introduced as representatives of five families: Beba- Elza, Cura- Lela, Lenica- Lasta, Liza - Cica and Liza - Pliva. Characterising of mitochondrial genome has confirmed negligent record keeping of origin in private breeding. Some non-recognised families are descendants of recognised Lipizzan families. Haplotype Allegra is found in the family of Liza-Cica-Pliva, and haplotype Capriola in the family of Cura-Lela. Both haplotypes are very common in the Lipizzaner breed and inherent to classic Lipizzan families.
The Istrian breed of cattle, of the Yugoslavian peninsula of that name, is an endangered species. The Istrian breed is related to other Podolic breeds and research into its genetic relationships with ...other breeds, as well as its conservation through the establishment of open nucleus units, frozen embryo stocks and a semen bank is being undertaken.
Istarsko govedo najbrojnija je autohtona pasmina goveda u Hrvatskoj. Predstavlja živi spomenik vremena i podneblja o kojem gotovo dva desetljeća brinu vrijedni uzgajivači i šira društvena zajednica. ...Zaustavljena su negativna kretanja u populaciji istarskog goveda izazvana globalnim trendovima u animalnoj proizvodnji gospodarski razvijenijih društava, a aktualna populacija nalazi se u fazi oporavka i blage stagnacije. Usporavanje daljnjeg rasta populacije uvjetovano je izostankom provjerene gospodarske reafirmacije koja treba interesno povezati uzgajivače i potrošače, osiguravajući time samoodrživost pasmine. Pokrenuti projekt reafirmacije istarskog goveda kroz program proizvodnje ''Mesa istarskog goveda'' potakao je konsenzus svih subjekata u slijedu namirnice (uzgoj, procesing, gastronomija), te daje prve pozitivne učinke.
