1 Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Avda. de Medina Azahara 7, 14005 Córdoba, Spain
2 Centro de Biología Molecular ...(CSIC-UAM), Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain
and 3 Institute for Animal Health, Pirbright Laboratory, Pirbright, Surrey, UK
In order to determine the cause of cellular death of lymphocytes in pigs with acute African swine fever and the relationships between African swine fever virus (ASFV) and interstitial cells, ten pigs were inoculated with a highly virulent strain of ASFV (Malawi '83) and samples taken for ultrastructural study of hepatic and renal interstitial tissues. We demonstrated death by apoptosis of lymphocytes and virus replication in fibroblasts, smooth muscle cells and endothelial cells in the interstitial tissues of pigs inoculated with ASFV. From day 5 onwards, apoptotic lymphocytes and intense virus replication in hepatic interstitial macrophages and fibroblasts were observed. By day 7, apoptotic lymphocytes and virus replication in macrophages, interstitial capillary endothelial cells and fibroblasts in the kidney were observed. Virus replication was also seen in smooth muscle cells of hepatic and renal arterioles and venules. Our results suggest that mononuclear phagocyte system (MPS) cell activation, and the resulting release of cytokines, could induce apoptosis of lymphocytes and virus replication in non-MPS cells.
* Author for correspondence. Fax +34 57 218666. e-mail cc0gralf@lucano.uco.es
Received 31 January 1995;
accepted 3 May 1995.
This article reports an outbreak of fatal cryptosporidiosis in captive ferrets (Mustela putorius furo) that was diagnosed by structural and ultrastructural studies. The ferrets were infected by ...Cryptosporidium sp. from goats and the fatal course of this outbreak may have been related to stress.
Tissues obtained from pigs inoculated with African swine fever virus (ASFV), fixed by vascular perfusion using glutaraldehyde, and embedded in paraffin or araldite were used for an immunohistologic ...electron microscopic study. To detect ASFV antigens, 4 methods were used on paraffin sections with or without pretreatment of the tissues. Use of biotinylated anti-ASFV antiserum combined with avidin -biotin complex and peroxidase proved to be the most suitable method, and antigen was detected in tissues infected with 2 ASF viruses of different virulence. Use of the glutaraldehyde fixation method should ensure optimal morphologic (structural and ultrastructural) data while allowing an immunohistologic study, and add to knowledge of the pathogenesis of ASF
The use of immunohistochemical (IHC) techniques in routinely processed tissue samples has greatly increased during the last decade, particularly in human oncology, due to both the higher capacity of ...newer IHC techniques to amplify lower antigenic signals and the development of monoclonal and polyclonal antibodies to several tumor-associated as well as the applications of these IHC techniques to tumor pathology in domestic animals. Several authors have confirmed the high cross-reactivity between human and animal proteins which serve as tumor markers, and also the feasibility of use of IHC techniques in Veterinary Pathology, as they allow the differentiation of tumors by cellular origin (intermediate filaments, synaptophysin) or cell type (endothelial, and histiocytic antigens, S-100 protein, carcinoembryonic antigen, alpha-fetoprotein). The present work represents a revision of those tumor tissue markers which have been shown to be most useful in diagnostic oncology
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