The analysis of tumor interstitial fluid (TIF) composition is a valuable procedure to identify antimetastatic targets, and different laboratories have set up techniques for TIF isolation and ...proteomic analyses. However, those methods had never been compared in samples from the same tumor and patient. In this work, we compared the two most used methods, elution and centrifugation, in pieces of the same biopsy samples of cutaneous squamous cell carcinoma (cSCC). First, we established that high G-force (10 000g) was required to obtain TIF from cSCC by centrifugation. Second, we compared the centrifugation method with the elution method in pieces of three different cSCC tumors. We found that the mean protein intensities based in the number of peptide spectrum matches was significantly higher in the centrifuged samples than in the eluted samples. Regarding the robustness of the methods, we observed higher overlapping between both methods (77–80%) than among samples (50%). These results suggest that there exists an elevated consistence of TIF composition independently of the method used. However, we observed a 3-fold increase of extracellular proteins in nonoverlapped proteome obtained by centrifugation. We therefore conclude that centrifugation is the method of choice to study the proteome of TIF from cutaneous biopsies.
(CA) infections have been associated with psoriasis onset or disease flares. However, the integrated immune response against this fungus is still poorly characterized in psoriasis. We studied ...specific immunoglobulins in plasma and the CA response in cocultures of circulating memory CD45RA
cutaneous lymphocyte antigen (CLA)
T cell with autologous epidermal cells from plaque and guttate psoriasis patients (cohort 1,
= 52), and also healthy individuals (
= 17). A complete proteomic profile was also evaluated in plaque psoriasis patients (cohort 2,
= 114) regarding their anti-CA IgA levels. Increased anti-CA IgA and IgG levels are present in the plasma from plaque but not guttate psoriasis compared to healthy controls. CA cellular response is confined to CLA
T cells and is primarily Th17. The levels of anti-CA IgA are directly associated with CLA
Th17 response in plaque psoriasis. Proteomic analysis revealed distinct profiles in psoriasis patients with high anti-CA IgA. C-C motif chemokine ligand 18, chitinase-3-like protein 1 and azurocidin were significantly elevated in the plasma from plaque psoriasis patients with high anti-CA levels and severe disease. Our results indicate a mechanism by which
exposure can trigger a clinically relevant IL-17 response in psoriasis. Assessing anti-CA IgA levels may be useful in order to evaluate chronic psoriasis patients.
Summary
Background
A dramatic rise in the incidence rates of basal cell carcinoma (BCC) in young women has been reported.
Objectives
We investigate potential risk factors (RF) for sporadic BCC in ...young patients and the current distribution of such RF in the general population of Catalonia, comparing the differences among men and women.
Patients and Methods
A case‐control study was performed, 69 BCCs diagnosed in patients ≤ 45 years of age vs. 69 healthy controls. Afterward, 1,078 participants from the general population completed an RF questionnaire.
Results
Repeated sunburns were more frequent in instances of early‐onset BCC in covered skin than in sun‐exposed skin (P = 0.029). In the general population, 39.1 % of participants reported sunbed use (50.1 % in women, 10.9 % in men). Sunbed use was the only relevant RF more predominant in women than men, favoring the trend to female predominance of BCCs above other RF. Additionally, we found a significant trend in young participants for reduced sunbed use (P < 0.001), although they had the same percentage of repeated sunburns. Repeated sunburns are the most relevant RF for early‐onset BCCs that can be targeted in prevention campaigns.
Conclusions
We should be aware of the more relevant RF for early‐onset BCCs and their distribution among the general population to address preventive campaigns.
Zusammenfassung
Hintergrund
Es gibt Berichte über einen dramatischen Anstieg der Inzidenz von Basalzellkarzinomen (BZK) bei jungen Frauen.
Ziele
Wir untersuchten potenzielle Risikofaktoren (RF) für ...sporadische BZK bei jungen Patienten sowie die gegenwärtige Verteilung dieser RF in der Allgemeinbevölkerung Kataloniens. Dabei verglichen wir Männer und Frauen.
Patienten und Methodik
Es wurde eine Fall‐Kontroll‐Studie mit 69 Patienten ≤ 45 Jahren mit BZK‐Diagnose und 69 gesunden Kontrollen durchgeführt. Anschließend füllten 1078 Teilnehmer aus der Allgemeinbevölkerung einen RF‐Fragebogen aus.
Ergebnisse
Wiederholte Sonnenbrände waren bei BZK mit frühem Beginn an bedeckter Haut häufiger als an sonnenexponierten Hautbereichen (p = 0,029). In der Allgemeinbevölkerung gaben 39,1 % der Teilnehmer an, Solarien zu nutzen (50,1 % der Frauen, 10,9 % der Männer). Die Solariumnutzung war der einzige relevante RF, der bei Frauen häufiger auftrat als bei Männern und dementsprechend den Trend zu einer weiblichen Prädominanz bei BZK mehr als andere RF begünstigt. Zudem ermittelten wir bei jungen Teilnehmern einen signifikanten Trend hin zu verringerter Solariumnutzung (p < 0,001). Der Prozentsatz wiederholter Sonnenbrände war jedoch unverändert. Wiederholter Sonnenbrand war der wichtigste RF für BZK mit frühem Beginn, der mittels Präventionskampagnen gezielt angegangen werden kann.
Schlussfolgerungen
Wir sollten uns der wichtigsten RF für BZK mit frühem Beginn sowie ihrer Verteilung in der Allgemeinbevölkerung bewusst sein, um sie in Präventionskampagnen anzugehen.
Background. Snow reflectivity and altitude increase the exposure of ski resort workers to solar ultraviolet radiation. The aim was to assess the presence of skin cancer in ski resorts workers and ...compare it with other groups of outdoor workers reviewing published studies. Methods. An observational cross-sectional prospective study was conducted in the three largest ski resorts in Spain: Baqueira Beret, Lleida; Formigal, Huesca and Sierra Nevada, Granada. All outdoor workers including ski instructors were invited to participate in the study. The participants completed a validated questionnaire about sun exposure and underwent a skin examination. Results. 219 workers were included in the study (80% male; mean age 43.8 (SD 11.31) years). Actinic keratosis (AK) but no other skin cancers were detected in 32 participants (14.62%). Those with AK worked in the Southernmost ski resort, were more likely to have light colour hair, and were older and with higher photoaging grade than those without them. Conclusion. Compared to other studies, outdoor workers on ski resorts show a higher prevalence of AK than general population but a lower prevalence than other groups of outdoor workers.
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